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Popescu C.P.,Carol Davila University of Medicine and Pharmacy | Popescu C.P.,Dr Victor Babes Clinical Hospital Of Infectious And Tropical Diseases | Popescu C.P.,European Society of Clinical Microbiology and Infection Study Group for Infectious Diseases of the Brain | Florescu S.A.,Carol Davila University of Medicine and Pharmacy | And 10 more authors.
Emerging Infectious Diseases | Year: 2017

We characterized influenza B virus-related neurologic manifestations in an unusually high number of hospitalized adults at a tertiary care facility in Romania during the 2014-15 influenza epidemic season. Of 32 patients with a confirmed laboratory diagnosis of influenza B virus infection, neurologic complications developed in 7 adults (median age 31 years). These complications were clinically diagnosed as confirmed encephalitis (4 patients), possible encephalitis (2 patients), and cerebellar ataxia (1 patient). Two of the patients died. Virus sequencing identified influenza virus B (Yam)-lineage clade 3, which is representative of the B/Phuket/3073/2013 strain, in 4 patients. None of the patients had been vaccinated against influenza. These results suggest that influenza B virus can cause a severe clinical course and should be considered as an etiologic factor for encephalitis. © 2017, Centers for Disease Control and Prevention (CDC). All Rights Reserved.


PubMed | Carol Davila University of Medicine and Pharmacy, University of Medicine and Pharmacy of Craiova, National Institute of Research Cantacuzino, Romanian Academy of Sciences and 2 more.
Type: | Journal: Microbial pathogenesis | Year: 2016

Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe infections versus asymptomatic carriage in order to identify clinically relevant bacterial traits that could easily be detected in clinical practice and could be suggestive for particular host-pathogen interactions such as cyto-adhesion or biofilm formation, ultimately orienting the clinical decision-making process. We have used invitro phenotypic methods to assess adhesion to and invasion of eukaryotic cells, biofilm development, and expression of soluble virulence factors in 92 Staphylococcus spp. strains. The adhesion index, invasion capacity, biofilm formation and expression of soluble factors did not differ significantly between clinical and commensal strains. The major bacterial traits we found to be significantly more prevalent in clinical staphylococci were the aggregative adhesion pattern (P=0.012), cluster adhesion (P=0.001) and tetrad morphology (P=0.018). The aggregative adhesion pattern was correlated with higher cyto-adhesion (P<0.001), higher invasion capacity (P=0.003) and lower Carmeli scores (P=0.002). Three major bacterial traits, namely tetrad morphology, aggregative adhesion pattern, and resistance to methicillin (acronym: TAM), can be used to compute an aggressiveness score (SAS) predictive of the staphylococcal strains virulence and capacity to initiate and develop a biofilm-driven chronic infectious process versus a fulminant acute infection, in a susceptible host.


Negut A.C.,Carol Davila University of Medicine and Pharmacy | Negut A.C.,National Institute For Infectious Diseases Prof Dr Matei Bals | Chifiriuc M.-C.,University of Bucharest | Sandulescu O.,Carol Davila University of Medicine and Pharmacy | And 17 more authors.
FEMS Microbiology Letters | Year: 2016

The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria. We have assessed the influence of commercially available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages: 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9-fold for STAPHYLOCOCCAL; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations (P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially available bacteriophages. We have also ascertained there is phage-driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections. © FEMS 2016. All rights reserved.


Mihalcea A.,National Institute of Research Cantacuzino | Mihalcea A.,Polytechnic University of Bucharest | Onu A.,National Institute of Research Cantacuzino | Tucureanu C.,National Institute of Research Cantacuzino | And 4 more authors.
Revista de Chimie | Year: 2015

Yeasts are an important source of natural carotenoids (a group of oil soluble pigments). These solubilization characteristics of carotenoids have led to studies on recovery of these pigments from vegetable oils such as sunflower oil, soy oil, Palm oil, etc. Torularhodin is one of the carotenoid pigments produced by the yeast Rhodotorula sp., with a terminal carboxylic group considered now-a-days as a powerful antioxidant In this paper we extract the torularhodin from Rhodotorula rubra yeast using sunflower oil. Approximately 97% of the cellular mass is disintegrated over five cycles through the high pressure IKA WERKE homogenizer at a pressure from 1500 to 2000 bar. The HPLC analysis proves a good capacity for extracting the total carotenoid pigments in sunflower oil by obtaining a high value of 958.68 μg/mL medium from which a percentage of 70.29 % is represented by torularhodin.

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