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Zhang Y.,Peking University | Wang Q.,Peking University | Wang T.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Zhang H.,Xingtai Peoples Hospital | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2012

A water-soluble polysaccharide (WATP), with a molecular weight of 6.3×104Da, was isolated from Aster tataricus. According to gas chromatography (GC) analysis, WATP was composed of galactose, glucose, fucose, rhamnose, arabinose and mannose with molar ratios of 2.1:1.3:0.9:0.5:0.3:0.6. The effects of WATP on cell proliferation and apoptosis in human gastric cancer SGC-7901 cells were examined. MTT assay showed that WATP had a perfectly tumor growth inhibitory activity on SGC-7901 cells, but no cytotoxicity on SGC-7901 and primary human polymorphonuclear (PMN) cells analyzed using LDH assay. Flow cytometry analysis indicated that WATP could significantly induce apoptosis of SGC-7901 cells. Furthermore using Rh123 and Fluo-3 as fluorescent probes, respectively, it was found that mitochondrial transmembrane potential (ΔΨm) of treatment groups was significantly lower than that in un-treatment group and the concentration of calcium in cells exposed to WATP for 24h was increased in a dose dependent manner compared with unexposed group. These results suggest that WATP induces apoptosis of SGC-7901 cells through calcium- and ΔΨm-dependent pathways, indicating that it is potentially useful as a natural anti-cancer agent. © 2012 Elsevier B.V. Source


Zhang Q.-L.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Tian D.-A.,Huazhong University of Science and Technology | Xu X.-J.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province
Onkologie | Year: 2011

Background: Pokemon is a transcription repressor which plays a critical role in cell transformation and malignancy. However, little is known about its effect on the development and progression of hepatocellular carcinoma (HCC). The aim of this study was to investigate the expression of Pokemon in human HCC tissues and the biological behavior of Pokemon in HCC cells in which it is overexpressed. We also explored the expression of potential downstream cofactors of Pokemon. Materials and Methods: Reverse transcription polymerase chain reaction and Western blot analysis were used to investigate the expression of Pokemon in tissues of 30 HCC patients. We then examined cell proliferation or apoptosis and β-catenin or H-ras expression in Pokemon-depleted HepG 2 cells using DNA vector-based RNA interference technology. Results: Pokemon was markedly expressed in 22/30 (73.3%) HCC tissues, with expression levels higher than in adjacent normal liver tissues (p < 0.05); expression is correlated with tumor size. In contrast, depletion of Pokemon inhibited proliferation of HepG 2 or induced apoptosis. Also, H-ras expression decreased to a large extent. Conclusions: Pokemon exerts its oncogenic activity in the development of HCC by promoting cancer cell growth and reducing apoptosis, and the effect may be mediated by H-ras. © 2011 S. Karger AG, Basel. Source


Zhang Q.-L.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Xing X.-Z.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Li F.-Y.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Xing Y.-J.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province | Li J.,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province
Oncology Research and Treatment | Year: 2015

We firstly investigated the expression of Pokemon in patients with non-small cell lung cancer (NSCLC), then characterized the role of Pokemon in evaluating the response to combined cisplatin and paclitaxel chemotherapy and prognosis. Patients and Methods: In this study, 61 patients with previously untreated locally advanced or metastatic NSCLC were treated with a combination chemotherapy comprising cisplatin and paclitaxel. The correlation between serum expression of Pokemon and effectiveness of chemotherapy was assessed. Results: The expression level of Pokemon in NSCLC patients was higher than that in healthy controls (p = 0.000), and was correlated with tumor size and TNM stage (p < 0.05). Kaplan-Meier analysis and Cox proportional hazard model demonstrated a poor response and shorter survival time in patients with pretreatment Pokemon levels in excess of 135.09 ng/ml compared to those with Pokemon levels below 135.09 ng/ml (p = 0.013). Pokemon ≥ 135.09 ng/ml was an independent risk factor for survival time in NSCLC patients undergoing combination chemotherapy (p = 0.018). Conclusion: The serum level of Pokemon correlated with efficacy of cisplatin and paclitaxel combination chemotherapy and survival time, which indicated that Pokemon may be a potentially useful biomarker for predicting treatment effectiveness of first-line chemotherapy and prognosis in NSCLC. © 2015 S. Karger GmbH, Freiburg. Source

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