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Wei X.,Cangzhou City Central Hospital | Wei S.,Cangzhou City Central Hospital | Kong Y.,Cangzhou City Central Hospital | Tian S.,Cangzhou City Central Hospital | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2017

Omentin is a visceral fat-derived adipokine associated with insulin sensitivity. Insulin resistance is a major hallmark of liver cirrhosis. This study aimed to assess the association of serum omentin and adiponectin levels with the occurrence of liver cirrhosis. Seventy-nine patients with liver cirrhosis and 27 healthy controls were enrolled at Cangzhou Central Hospital between March 2014 and June 2015. ELISA was performed to assess serum omentin and adiponectin levels. Prothrombin time (PT), alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin (TBIL), serum albumin (ALB), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and fasting plasma glucose (FPG) were assessed using an automatic biochemistry analyzer. Fasting insulin (FIns) was determined by immunoassays. Correlations of omentin and adiponectin levels with the above biochemical indicators were evaluated (Spearman method). Sensitivity and specificity of omentin and adiponectin in predicting the occurrence of liver cirrhosis were determined using ROC curves. Results showed that omentin and adiponectin levels were significantly higher in patients with liver cirrhosis than controls (P<0.05). Areas under the ROC curves of omentin and adiponectin were 0.841 and 0.939, respectively, with cutoff values for liver cirrhosis prediction of 901 ng/L and 764 µg/L, respectively. However, omentin and adiponectin levels were not significantly correlated with HOMA-IR and insulin levels. Omentin and adiponectin levels were positively correlated. In conclusion, omentin and adiponectin levels are elevated in patients with liver cirrhosis and could be considered indicators for predicting liver cirrhosis, but they are not associated with insulin resistance in patients with liver cirrhosis. © 2017, E-Century Publishing Corporation. All rights reserved.


Yang M.,Cangzhou City Central Hospital | Xiao L.-B.,Cangzhou City Central Hospital | Gao Z.-S.,Cangzhou City Central Hospital | Zhou J.-W.,Cangzhou City Central Hospital
Medical Science Monitor | Year: 2017

Background: Coronary artery bypass grafting (CABG) is a common procedure to circumvent the obstruction of coronary arteries when stents are unsuitable. CABG is a very traumatic surgery that requires redirecting blood flow to an external pump. Thus, this procedure has many risks during and after surgery, and minimizing these risks would greatly benefit the patients. Material/Methods: We selected 126 patients with coronary artery syndrome and who were unsuitable for stent percutaneous coronary intervention. The observation group received minimally invasive direct coronary artery bypass (MIDCAB), while the control group was treated with off-pump CABG. Results: Blood markers and echocardiography before and after treatment improved equally in both groups. Neither group exhibited obvious adverse reactions, or liver and kidney function damage. However, surgical bleeding and postoperative observation days were significantly reduced in the MIDCAB group. Death and cardiac shock at the end of follow-up were significantly lower in the MIDCAB group. Conclusions: Overall, the clinical benefits of MIDCAB and OP-CABG were similar, but MIDCAB significantly reduced postoperative hospital stay and intraoperative blood transfusion, and improved clinical prognosis. © Med Sci Monit.


Xiao L.,Cangzhou City Central Hospital | Zhang Y.,Cangzhou City Central Hospital | Yang M.,Cangzhou City Central Hospital | Zhou J.,Cangzhou City Central Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To investigate the clinical effect of minimally invasive direct coronary artery bypass (MIDCAB) in left chest with percutaneous coronary intervention (PCI) in the treatment of coronary heart disease. Methods: 100 cases of patients with coronary heart disease were randomly divided into observation group (50 cases) and control group (50 cases). The patients in observation group firstly underwent MIDCAB, and then underwent selective PCI to treat right coronary artery and vascular disease; the control group underwent traditional CABG. Indicators such as preoperative basic condition, postoperative drainage volume in 24 h, mechanical ventilation time, ICU monitoring time, hospital days, re-exploration for mediastinal bleeding, peri-operative myocardiac infarction and fatality rate etc. were compared between two groups. The occurrence of cardiovascular adverse events during follow-up period was also observed. Results: There were no significant differences of age, sex, history of diabetes and hypertension, the proportion of dyslipdemia, smoking, drinking, angina pectoris, and cardiac functional (NHYA classification) in the two groups of patients (P>0.05). Patients were followed-up for 3 months after operation; we found that the LVEF in observation group was obviously higher than that in control group and the difference had statistical significance (P<0.05). Compared with control group, the postoperative drainage volume in 24 h, mechanical ventilation time, CICU monitoring time, postoperative hospital stay, operative time and the length of incision were significantly less in observation group, which had statistical difference (P<0.05). There was no re-exploration for mediastinal bleeding or death in two groups. There was no significant difference in pleural effusion and infection between the two groups (P>0.05). The results of one-year follow-up showed that there were 2 cases of recurrence of myocardial infarction in the control group; however, there was no cardiac death or recurrence of myocardial infarction, or second CABG/PCI in observation group. Conclusion: For suitable patients with coronary heart disease, MIDCAB with PCI surgery is safe and reliable, and has a significant short-term clinical effect. © 2016, E-Century Publishing Corporation. All rights reserved.


Xing B.-H.,Hebei Medical University | Yang F.-Z.,Cangzhou City Central Hospital | Wu X.-H.,Hebei Medical University
Journal of Pharmacological Sciences | Year: 2016

Gestational diabetes mellitus (GDM) is a disease commonly occurs during mid to late pregnancy with pathologies such as hyperglycemia, hyperinsulinemia and mal-development of fetus. We have previously demonstrated that pancreatic endoderm (PE) derived from human embryonic stem cells (hESCs) effectively alleviated diabetic symptoms in a mouse model of GDM, although the clinical efficacy was limited due to oxidative stress. In this study, using the anti-oxidant agent naringenin, we aimed to further enhance the efficacy of hESC-derived PE transplant. Insulin-secreting PE was differentiated from hESCs, which were then transplanted into GDM mice. Naringenin was administered to mice receiving the PE transplant, with sham operated mice serving as negative control, to assess its effect on alleviation of GDM symptoms. We found that naringenin supplement further improved insulin response, glucose metabolism and reproductive outcome of the PE-transplanted female mice. Our new findings further potentiates the feasibility of using differentiated hESCs to treat GDM, in which anti-oxidative agent such as naringenin could greatly enhance the clinical efficacy of stem cell based therapies. © 2016 The Authors.


PubMed | Cangzhou City Central Hospital and Hebei Medical University
Type: Journal Article | Journal: Journal of pharmacological sciences | Year: 2016

Gestational diabetes mellitus (GDM) is a disease commonly occurs during mid to late pregnancy with pathologies such as hyperglycemia, hyperinsulinemia and mal-development of fetus. We have previously demonstrated that pancreatic endoderm (PE) derived from human embryonic stem cells (hESCs) effectively alleviated diabetic symptoms in a mouse model of GDM, although the clinical efficacy was limited due to oxidative stress. In this study, using the anti-oxidant agent naringenin, we aimed to further enhance the efficacy of hESC-derived PE transplant. Insulin-secreting PE was differentiated from hESCs, which were then transplanted into GDM mice. Naringenin was administered to mice receiving the PE transplant, with sham operated mice serving as negative control, to assess its effect on alleviation of GDM symptoms. We found that naringenin supplement further improved insulin response, glucose metabolism and reproductive outcome of the PE-transplanted female mice. Our new findings further potentiates the feasibility of using differentiated hESCs to treat GDM, in which anti-oxidative agent such as naringenin could greatly enhance the clinical efficacy of stem cell based therapies.


Xing B.,Cangzhou City Central Hospital | Wang L.,Cangzhou City Peoples Hospital | Li Q.,Cangzhou City Central Hospital | Cao Y.,Cangzhou City Central Hospital | And 3 more authors.
Nutrition Research | Year: 2015

Gestational diabetes mellitus is a condition commonly encountered during mid to late pregnancy with pathologic manifestations including hyperglycemia, hyperinsulinemia, insulin resistance, and fetal maldevelopment. The cause of gestational diabetes mellitus can be attributed to both genetic and environmental factors, hence complicating its diagnosis and treatment. Pancreatic progenitors derived from human embryonic stem cells were shown to be able to effectively treat diabetes in mice. In this study, we have developed a system of treating diabetes using human embryonic stem cell-derived pancreatic endoderm in a mouse model of gestational diabetes mellitus. Human embryonic stem cells were differentiated in vitro into pancreatic endoderm, which were then transplanted into db/+ mice suffering from gestational diabetes mellitus. The transplant greatly improved glucose metabolism and reproductive outcome of the females compared with the control groups. Our findings support the feasibility of using differentiated human embryonic stem cells for treating gestational diabetes mellitus patients. © 2015 Elsevier Inc.


PubMed | Cangzhou City Peoples Hospital, Cangzhou City Central Hospital and Bethune International Peace Hospital
Type: Journal Article | Journal: Journal of physiology and biochemistry | Year: 2015

Gestational diabetes mellitus (GDM) has emerged as an epidemic disease during the last decade, affecting about 2 to 5% pregnant women. Even among women who have gestational hyperglycemia may also be positively related to adverse outcomes as GDM. Since heat shock protein (Hsp) 70 has been reported to be associated with diabetes and insulin resistance and its expression was reported to be negatively regulated by the membrane-permeable Hsp70 inhibitor MAL3-101 while positively regulated by the Hsp70 activator BGP-15, we investigated whether Hsp70 played a role in a gestational hyperglycemia mouse model. Mice were divided into non-pregnant and pregnant groups, and each comprised three subgroups: control, high-fat diet (HFD) + MAL3-101, and HFD + BGP-15. We examined the serum levels of triglycerides, total cholesterol, glucose, and insulin, as well as conducted thermal detection of brown adipose tissue (BAT). The role of Hsp70 in BAT apoptosis was also investigated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and caspase-3 staining. Higher serum level of Hsp70 was associated with increased bodyweight gain after pregnancy in mice fed HFD. Circulating Hsp70 was elevated in control pregnant mice compared to control non-pregnant mice. BGP-induced serum Hsp70 expression reduced triglycerides, total cholesterol, glucose, and insulin levels in the serum. Additionally, thermal detection of BAT, TUNEL, and caspase-3 staining revealed relationship correlation between Hsp70 and BAT functions. Hsp70 level is associated with hyperglycemia during pregnancy. Our results support the role of Hsp70 in facilitating BAT activities and protecting BAT cells from apoptosis via caspase-3 pathway.


PubMed | Cangzhou City Peoples Hospital, Cangzhou City Central Hospital and Bethune International Peace Hospital
Type: Evaluation Studies | Journal: Nutrition research (New York, N.Y.) | Year: 2015

Gestational diabetes mellitus is a condition commonly encountered during mid to late pregnancy with pathologic manifestations including hyperglycemia, hyperinsulinemia, insulin resistance, and fetal maldevelopment. The cause of gestational diabetes mellitus can be attributed to both genetic and environmental factors, hence complicating its diagnosis and treatment. Pancreatic progenitors derived from human embryonic stem cells were shown to be able to effectively treat diabetes in mice. In this study, we have developed a system of treating diabetes using human embryonic stem cell-derived pancreatic endoderm in a mouse model of gestational diabetes mellitus. Human embryonic stem cells were differentiated in vitro into pancreatic endoderm, which were then transplanted into db/+ mice suffering from gestational diabetes mellitus. The transplant greatly improved glucose metabolism and reproductive outcome of the females compared with the control groups. Our findings support the feasibility of using differentiated human embryonic stem cells for treating gestational diabetes mellitus patients.


Guo Q.,Cangzhou City Central Hospital | Li R.,Cangzhou City Central Hospital | Wang L.,Cangzhou City Central Hospital | Zhang D.,Cangzhou City Central Hospital | Ma Y.,Cangzhou City Central Hospital
International Journal of Clinical and Experimental Medicine | Year: 2015

Background: Transversus abdominis plane (TAP) block and local anaesthetic wound infiltration can provide effective pain relief at the wound site after surgery. However, the relative efficacy of two techniques for postoperative analgesia remains controversial. Methods: We searched PUBMED, EMBASE and CENTRAL databases for randomized controlled trials (RCTs) comparing TAP block with wound infiltration for pain relief after surgery. The primary outcomes were pain scores at rest and on movement at 1, 8 and 24 hours postoperatively and cumulative morphine consumption over 24 hours. The secondary outcomes were time to first rescue analgesic, number of rescue analgesic use and opioids-related side-effects. Results: Nine RCTs with a total of 500 participants were included. TAP block was associated with significant lower rest and dynamic pain scores at 8 hour [MD = -1.08, 95% CI (-1.89-0.26), P = 0.009] and 24 hour [MD = -0.83, 95% CI (-1.60, -0.06), P = 0.03] postoperatively than wound infiltration, but no significant difference was found at 1 hour [MD = -0.94, 95% CI (-1.97, 0.09), P = 0.08] postoperatively. In adults, TAP block significantly reduced 24-hour overall morphine consumption by 3.85 mg [MD = -3.85, 95% CI (-7.47, -0.22), P = 0.04] compared with wound infiltration. Subgroup analysis showed that adults received TAP block appeared to have lower rest pain scores at 24 hour than children (P = 0.008). Conclusion: TAP block provides superior analgesia compared with wound infiltration in the setting of a multimodal analgesic regimen. Subgroup analysis indicated that adults may have benefits additional to the analgesic effect than children. © 2015, International Journal of Clinical and Experimental Medicine. All rights reserve.


PubMed | Cangzhou City Central Hospital
Type: | Journal: Reproductive sciences (Thousand Oaks, Calif.) | Year: 2016

Preeclampsia (PE) is a pregnancy-specific condition characterized by new-onset hypertension. There is evidence suggesting that imbalances of angiogenic factors, oxidative stress, and inflammation may be central to the pathogenesis of PE. We sought to investigate whether simvastatin would reduce mean arterial pressure, restore the angiogenic balance, and ameliorate inflammation and oxidative stress in a nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester (l-NAME)-induced rat model of PE. We found that blood pressure was significantly increased in the l-NAME group compared to normal pregnant dams (P < .01), and simvastatin reduced this difference. In addition, dams from the l-NAME group showed lower vascular endothelial growth factor (VEGF) and interleukin (IL) 10 levels and higher plasma-soluble FMS-like tyrosine kinase 1 (sFlt-1), tumor necrosis factor (TNF-), and oxidative stress marker malondialdehyde (MDA) levels as compared to control dams (P < .01, for all). Interestingly, simvastatin treatment significantly increased VEGF and IL-10 levels while decreased sFlt-1, TNF-, and MDA levels compared to the untreated l-NAME group. Moreover, simvastatin treatment significantly upregulated protein expression of placental p-extracellular signal-regulated kinase (ERK1), p-p38 mitogen-activated protein kinase (MAPK), p-c-Jun N-terminal kinase, and p-protein kinase B compared to untreated l-NAME control. These results suggest that simvastatin treatment restores angiogenic balance and ameliorates inflammation and oxidative stress in a rat model of PE involving ERK/MAPK pathway.

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