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Xing B.-H.,Hebei Medical University | Yang F.-Z.,Cangzhou City Central Hospital | Wu X.-H.,Hebei Medical University
Journal of Pharmacological Sciences | Year: 2016

Gestational diabetes mellitus (GDM) is a disease commonly occurs during mid to late pregnancy with pathologies such as hyperglycemia, hyperinsulinemia and mal-development of fetus. We have previously demonstrated that pancreatic endoderm (PE) derived from human embryonic stem cells (hESCs) effectively alleviated diabetic symptoms in a mouse model of GDM, although the clinical efficacy was limited due to oxidative stress. In this study, using the anti-oxidant agent naringenin, we aimed to further enhance the efficacy of hESC-derived PE transplant. Insulin-secreting PE was differentiated from hESCs, which were then transplanted into GDM mice. Naringenin was administered to mice receiving the PE transplant, with sham operated mice serving as negative control, to assess its effect on alleviation of GDM symptoms. We found that naringenin supplement further improved insulin response, glucose metabolism and reproductive outcome of the PE-transplanted female mice. Our new findings further potentiates the feasibility of using differentiated hESCs to treat GDM, in which anti-oxidative agent such as naringenin could greatly enhance the clinical efficacy of stem cell based therapies. © 2016 The Authors.

Xing B.,Cangzhou City Central Hospital | Wang L.,Cangzhou City Peoples Hospital | Li Q.,Cangzhou City Central Hospital | Cao Y.,Cangzhou City Central Hospital | And 3 more authors.
Nutrition Research | Year: 2015

Gestational diabetes mellitus is a condition commonly encountered during mid to late pregnancy with pathologic manifestations including hyperglycemia, hyperinsulinemia, insulin resistance, and fetal maldevelopment. The cause of gestational diabetes mellitus can be attributed to both genetic and environmental factors, hence complicating its diagnosis and treatment. Pancreatic progenitors derived from human embryonic stem cells were shown to be able to effectively treat diabetes in mice. In this study, we have developed a system of treating diabetes using human embryonic stem cell-derived pancreatic endoderm in a mouse model of gestational diabetes mellitus. Human embryonic stem cells were differentiated in vitro into pancreatic endoderm, which were then transplanted into db/+ mice suffering from gestational diabetes mellitus. The transplant greatly improved glucose metabolism and reproductive outcome of the females compared with the control groups. Our findings support the feasibility of using differentiated human embryonic stem cells for treating gestational diabetes mellitus patients. © 2015 Elsevier Inc.

Xing B.,Cangzhou City Central Hospital | Wang L.,Cangzhou City Peoples Hospital | Li Q.,Cangzhou City Central Hospital | Cao Y.,Cangzhou City Central Hospital | And 3 more authors.
Journal of Physiology and Biochemistry | Year: 2015

Gestational diabetes mellitus (GDM) has emerged as an epidemic disease during the last decade, affecting about 2 to 5 % pregnant women. Even among women who have gestational hyperglycemia may also be positively related to adverse outcomes as GDM. Since heat shock protein (Hsp) 70 has been reported to be associated with diabetes and insulin resistance and its expression was reported to be negatively regulated by the membrane-permeable Hsp70 inhibitor MAL3-101 while positively regulated by the Hsp70 activator BGP-15, we investigated whether Hsp70 played a role in a gestational hyperglycemia mouse model. Mice were divided into non-pregnant and pregnant groups, and each comprised three subgroups: control, high-fat diet (HFD) + MAL3-101, and HFD + BGP-15. We examined the serum levels of triglycerides, total cholesterol, glucose, and insulin, as well as conducted thermal detection of brown adipose tissue (BAT). The role of Hsp70 in BAT apoptosis was also investigated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and caspase-3 staining. Higher serum level of Hsp70 was associated with increased bodyweight gain after pregnancy in mice fed HFD. Circulating Hsp70 was elevated in control pregnant mice compared to control non-pregnant mice. BGP-induced serum Hsp70 expression reduced triglycerides, total cholesterol, glucose, and insulin levels in the serum. Additionally, thermal detection of BAT, TUNEL, and caspase-3 staining revealed relationship correlation between Hsp70 and BAT functions. Hsp70 level is associated with hyperglycemia during pregnancy. Our results support the role of Hsp70 in facilitating BAT activities and protecting BAT cells from apoptosis via caspase-3 pathway. © 2015, University of Navarra.

Xiao L.,Cangzhou City Central Hospital | Zhang Y.,Cangzhou City Central Hospital | Yang M.,Cangzhou City Central Hospital | Zhou J.,Cangzhou City Central Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Objective: To investigate the clinical effect of minimally invasive direct coronary artery bypass (MIDCAB) in left chest with percutaneous coronary intervention (PCI) in the treatment of coronary heart disease. Methods: 100 cases of patients with coronary heart disease were randomly divided into observation group (50 cases) and control group (50 cases). The patients in observation group firstly underwent MIDCAB, and then underwent selective PCI to treat right coronary artery and vascular disease; the control group underwent traditional CABG. Indicators such as preoperative basic condition, postoperative drainage volume in 24 h, mechanical ventilation time, ICU monitoring time, hospital days, re-exploration for mediastinal bleeding, peri-operative myocardiac infarction and fatality rate etc. were compared between two groups. The occurrence of cardiovascular adverse events during follow-up period was also observed. Results: There were no significant differences of age, sex, history of diabetes and hypertension, the proportion of dyslipdemia, smoking, drinking, angina pectoris, and cardiac functional (NHYA classification) in the two groups of patients (P>0.05). Patients were followed-up for 3 months after operation; we found that the LVEF in observation group was obviously higher than that in control group and the difference had statistical significance (P<0.05). Compared with control group, the postoperative drainage volume in 24 h, mechanical ventilation time, CICU monitoring time, postoperative hospital stay, operative time and the length of incision were significantly less in observation group, which had statistical difference (P<0.05). There was no re-exploration for mediastinal bleeding or death in two groups. There was no significant difference in pleural effusion and infection between the two groups (P>0.05). The results of one-year follow-up showed that there were 2 cases of recurrence of myocardial infarction in the control group; however, there was no cardiac death or recurrence of myocardial infarction, or second CABG/PCI in observation group. Conclusion: For suitable patients with coronary heart disease, MIDCAB with PCI surgery is safe and reliable, and has a significant short-term clinical effect. © 2016, E-Century Publishing Corporation. All rights reserved.

Ma L.,Fudan University | Liu C.,Hebei Provincial Childrens Hospital | Wang Y.,Baoding Womens and Childrens Hospital | Li S.,Handan Womens and Childrens Hospital | And 14 more authors.
Neonatology | Year: 2011

Dramatic progress has occurred in neonatal intensive care in tertiary centers in mid-eastern China. We investigated the characteristics of neonatal respiratory failure (NRF) including the incidence, management, outcomes and costs in 14 neonatal intensive care units (NICUs) of Hebei, a province at an intermediate economic level in China. Over a period of 12 consecutive months in 2007-2008, perinatal data were collected prospectively from all NICU admissions (n = 11,100). NRF was defined as severe hypoxemia requiring respiratory support for more than 24 h, and was diagnosed in 1,875 newborns (16.9%). The average birth weight of newborns with NRF was 2,200 g (range 600-5,500 g), with 60.9% <2,500 g, and 2% <1,000 g. The male:female ratio was 2.6:1. The leading diagnosis was respiratory distress syndrome; 58.3% of newborns with respiratory distress syndrome received surfactant. Continuous positive airway pressure was used more than ventilation (73.3 vs. 49.1%,p < 0.001). Overall, the mortality rate until discharge was 31.4% (583/1,859). Most deaths (432, 74.1%) followed a parental decision to withdraw care. NRF mortality varied in association with different gross domestic product levels, family annual income and nurse-to-bed ratios. The median cost of a hospital stay was 10,169 CNY (interquartile range: 6,745-16,386) for NRF survivors. We conclude that, despite the available respiratory support in these emerging NICUs, the mortality of NRF remains. This was associated with prematurity, standard of care but also with socioeconomic factors affecting treatment decisions. Assessment of efficacy of respiratory support for NRF in such emerging neonatal services should account for both standard of care and socioeconomic conditions. Copyright © 2010 S. Karger AG, Basel.

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