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Tian B.,Cangzhou Academy of Agricultural and Forestry science | Wang J.,Cangzhou Academy of Agricultural and Forestry science | Zhang L.,Cangzhou Academy of Agricultural and Forestry science | Li Y.,Cangzhou Academy of Agricultural and Forestry science | And 2 more authors.
Euphytica | Year: 2010

Lodging results in serious yield and quality reductions in foxtail millet (Setaria italica). Among summer crops, foxtail millet is a high density and soft stalked crop that is prone to lodging. Field selection for lodging resistance is difficult because the occurrence of lodging depends on the environmental conditions. A practical method for the evaluation of lodging is not available for foxtail millet, so an understanding of lodging-associated traits and identifying a suitable method to assess lodging are essential to select for lodging resistance and to predict the risk of lodging in a cultivar. In the present study, six stem and root traits associated with lodging was measured in the field and lodging coefficients were determined to evaluate lodging resistance in landrace and improved cultivars. Based on the results of correlation analysis, lodging coefficient is a suitable indicator for field selection for lodging resistance in both classes of cultivars tested for breeding purpose. Statistical analyses demonstrated that lodging was associated with stem quality rather than plant height and internode length at the stem base. The most important trait that directly impacted on lodging coefficient was mechanical strength of the stem and plant height in the landraces, while the weights of the above- and under-ground tissues, in combination with mechanical strength of the stem, were the most important contributors to lodging coefficient in the improved cultivars. © 2009 Springer Science+Business Media B.V.

Bi Y.,Chinese Academy of Agricultural Sciences | Zhang Y.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | Crickmore N.,University of Sussex | And 4 more authors.
Applied Microbiology and Biotechnology | Year: 2014

The Bacillus thuringiensis strain HBF-18 (CGMCC 2070), which has previously been shown to encode the cry8Ga toxin gene, is active against both Holotrichia oblita and Holotrichia parallela. Recombinant Cry8Ga however is only weakly toxic to these insect pests suggesting the involvement of additional toxins in the native strain. We report that through the use of Illumina sequencing three additional, and novel, genes, namely vip1Ad1, vip2Ag1, and cry8-like, were identified in this strain. Although no protein corresponding to these genes could be identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the HBF-18 proteome, reverse transcription (RT)-PCR indicated that all three genes were transcribed in the native strain. The two vip genes were cloned and expressed and, as with other Vip1/2 toxins, appeared to function as a binary toxin and showed strong activity against H. oblita, H. parallela and Anomala corpulenta. This is the first report to demonstrate that the Vip1/Vip2 binary toxin is active against these Scarabaeoidea larvae. The cry8-like gene appeared to be a C-terminally truncated form of a typical cry8 gene and was not expressed in our usual recombinant Bt expression system. When however the missing C-terminal region was replaced with the corresponding sequence from cry8Ea, the resulting hybrid expressed well and the toxin was active against the three test insects. © 2014, Springer-Verlag Berlin Heidelberg.

Liu J.,Chinese Academy of Agricultural Sciences | Yan G.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | Zhao C.,Chinese Academy of Agricultural Sciences | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2010

A newly engineered strain, denominated BIOT185, was constructed through integrating the cry8Ca2 gene into the endogenous plasmid of BT185 (contains cry8Ea1) by homologous recombination. The thermosensitive plasmid vector was eliminated by the rising temperature of recombinant cultures. No antibiotic gene or other unnecessary genes were introduced to the new strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis demonstrated that the cry8Ca2 gene was expressed normally and produced a 130-kDa protein in the BIOT185 strain. Bioassay results showed that the new strain had high toxicity to the pests Anomala corpulenta and Holotrichia parallela, which often damage the same fields. © 2010 Springer-Verlag.

Liu L.,China Agricultural University | Liu J.,China Agricultural University | Wang Q.,Cangzhou Academy of Agricultural and Forestry science | Ndayiragije P.,Burundi Academy of Agriculture Science | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Bactrocera (Bactrocera) invadens Drew (Diptera: Tephritidae) is a new species of fruit fly in 2005. It belongsto the Bactrocera dorsalis complex, but is difficult to diagnose based on solely morphological identification.It occurs in India, Bhutan and some countries of Africa. In this study, 14 adult samples of fruit flies werecollected from Bujumbura and Cibitoke in Burundi District. Microscopic observations showedmorphological character states that were congruent with the diagnosis of B. invadens. The mitochondrialDNA (mtDNA) cytochrome c oxidase I (COI) gene sequence alignment demonstrated the similarity betweenspecimens 1 and 2 and B. invadens is 99.47%, between specimen 3 and B. invadens 98.77%, betweenspecimen 4 and B. invadens 99.82%, and between the other 10 specimens and B. invadens 100%. Therefore,all samples were identified as B. invadens based on morphological characteristics and DNA barcode of COIgene. This study represented the first report of B. invadens in the Burundi District. © 2011 Academic Journals.

Shu C.,Chinese Academy of Agricultural Sciences | Tan S.,Chinese Academy of Agricultural Sciences | Yin J.,Chinese Academy of Agricultural Sciences | Soberon M.,National Autonomous University of Mexico | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2015

Holotrichia parallela is one of the most severe crop pests in China, affecting peanut, soybean, and sweet potato crops. Previous work showed that Cry8Ea toxin is highly effective against this insect. In order to identify Cry8Ea-binding proteins in the midgut cells of H. parallela larvae, we assembled a midgut tissue transcriptome by high-throughput sequencing and used this assembled transcriptome to identify Cry8Ea-binding proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS). First, we obtained de novo sequences of cDNAs from midgut tissue of H. parallela larvae and used available cDNA data in the GenBank. In a parallel assay, we obtained 11 Cry8Ea-binding proteins by pull-down assays performed with midgut brush border membrane vesicles. Peptide sequences from these proteins were matched to the H. parallela newly assembled midgut transcriptome, and 10 proteins were identified. Some of the proteins were shown to be intracellular proteins forming part of the cell cytoskeleton and/or vesicle transport such as actin, myosin, clathrin, dynein, and tubulin among others. In addition, an apolipophorin, which is a protein involved in lipid metabolism, and a novel membrane-bound alanyl aminopeptidase were identified. Our results suggest that Cry8Ea-binding proteins could be different from those characterized for Cry1A toxins in lepidopteran insects. © 2015, Springer-Verlag Berlin Heidelberg.

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