Cangzhou Academy of Agricultural and Forestry science

Cangzhou, China

Cangzhou Academy of Agricultural and Forestry science

Cangzhou, China
SEARCH FILTERS
Time filter
Source Type

Tian B.,Cangzhou Academy of Agricultural and Forestry science | Liu Y.,Cangzhou Academy of Agricultural and Forestry science | Zhang L.,Cangzhou Academy of Agricultural and Forestry science | Li H.,Chinese Academy of Agricultural Sciences
Crop and Pasture Science | Year: 2017

Foxtail millet (Setaria italica (L.) Beauv.) is a soft-stemmed summer cereal crop that is grown at a high crop density; however, stem lodging is recognised as a serious yield-limiting factor. The stem-breaking strength of the third to fifth basal internodes has previously been identified as the most important factor in determining the lodging resistance of foxtail millet. We measured variation in stem-breaking strength, length and weight of these internodes at different developmental stages and in response to different crop densities by using foxtail millet cultivars that differed in lodging resistance. The stem-breaking strength of the third internode was greater than of the fourth and fifth internodes, which had comparable stem-breaking strengths; this result was independent of genotype and developmental stage. The stem-breaking strengths of the three internodes were significantly correlated with each other and linearly related across different developmental stages and crop densities. The stem-breaking strength of the three internodes at hard dough stage (Zadoks growth stage Z87, at 30 days after flowering) was less than at other developmental stages in the lodging-resistant genotypes. Stem-breaking strength for the three internodes was correlated with fresh weight (FW) and dry weight (DW) per cm. The variation of FW and DW per cm of the internodes in response to crop density was attributed to the change of FW and DW of the corresponding internodes, rather than to variation in their length. Results from this study showed that the fourth or fifth basal internode was more prone to lodging than the third basal internode because of their lesser stem-breaking strength. Crop density linearly influenced the stem-breaking strength of the three basal internodes. Reducing crop density enhanced stem-breaking strength of third to fifth internodes, which may reduce the risk of stem lodging in this crop. Based on the findings, the stem-breaking strength of the fourth or fifth basal internode measured at the late grain-filling stage from Z77 (late milk) to Z87 (hard dough) differentiated stem-breaking strength, the most important stem lodging associated parameter, in the foxtail millet genotypes. © CSIRO 2017.


Jiang J.,Chinese Academy of Agricultural Sciences | Jiang J.,China Agricultural University | Huang Y.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | And 6 more authors.
Applied and Environmental Microbiology | Year: 2017

The Bacillus thuringiensis strain HBF-18 (CGMCC 2070), containing two cry genes (cry8-like and cry8Ga), is toxic to Holotrichia oblita larvae. Both Cry8-like and Cry8Ga proteins are active against this insect pest, and Cry8-like is more toxic. To analyze the characteristics of the binding of Cry8-like and Cry8Ga proteins to brush border membrane vesicles (BBMVs) in H. oblita larvae, binding assays were conducted with a fluorescent DyLight488-labeled Cry8-like toxin. The results of saturation binding assays demonstrated that Cry8-like bound specifically to binding sites on BBMVs from H. oblita, and heterologous competition assays revealed that Cry8Ga shared binding sites with Cry8-like. Furthermore, Cry8-like-binding proteins in the midgut from H. oblita larvae were identified by pulldown assays and liquid chromatographytandem mass spectrometry (LC-MS/MS). In addition, the H. oblita midgut transcriptome was assembled by high-throughput RNA sequencing and used for identification of Cry8-like-binding proteins. Eight Cry8-like-binding proteins were obtained from pulldown assays conducted with BBMVs. The LC-MS/MS data for these proteins were successfully matched with the H. oblita transcriptome, and BLASTX results identified five proteins as serine protease, transferrin-like, uncharacterized protein LOC658236 of Tribolium castaneum, ATPase catalytic subunit, and actin. These identified Cry8-like-binding proteins were different from those confirmed previously as receptors for Cry1A proteins in lepidopteran insect species, such as aminopeptidase, alkaline phosphatase, and cadherin. © 2017 American Society for Microbiology.


Liu L.,China Agricultural University | Liu J.,China Agricultural University | Wang Q.,Cangzhou Academy of Agricultural and Forestry science | Ndayiragije P.,Burundi Academy of Agriculture Science | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Bactrocera (Bactrocera) invadens Drew (Diptera: Tephritidae) is a new species of fruit fly in 2005. It belongsto the Bactrocera dorsalis complex, but is difficult to diagnose based on solely morphological identification.It occurs in India, Bhutan and some countries of Africa. In this study, 14 adult samples of fruit flies werecollected from Bujumbura and Cibitoke in Burundi District. Microscopic observations showedmorphological character states that were congruent with the diagnosis of B. invadens. The mitochondrialDNA (mtDNA) cytochrome c oxidase I (COI) gene sequence alignment demonstrated the similarity betweenspecimens 1 and 2 and B. invadens is 99.47%, between specimen 3 and B. invadens 98.77%, betweenspecimen 4 and B. invadens 99.82%, and between the other 10 specimens and B. invadens 100%. Therefore,all samples were identified as B. invadens based on morphological characteristics and DNA barcode of COIgene. This study represented the first report of B. invadens in the Burundi District. © 2011 Academic Journals.


Bi Y.,Chinese Academy of Agricultural Sciences | Zhang Y.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | Crickmore N.,University of Sussex | And 4 more authors.
Applied Microbiology and Biotechnology | Year: 2014

The Bacillus thuringiensis strain HBF-18 (CGMCC 2070), which has previously been shown to encode the cry8Ga toxin gene, is active against both Holotrichia oblita and Holotrichia parallela. Recombinant Cry8Ga however is only weakly toxic to these insect pests suggesting the involvement of additional toxins in the native strain. We report that through the use of Illumina sequencing three additional, and novel, genes, namely vip1Ad1, vip2Ag1, and cry8-like, were identified in this strain. Although no protein corresponding to these genes could be identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the HBF-18 proteome, reverse transcription (RT)-PCR indicated that all three genes were transcribed in the native strain. The two vip genes were cloned and expressed and, as with other Vip1/2 toxins, appeared to function as a binary toxin and showed strong activity against H. oblita, H. parallela and Anomala corpulenta. This is the first report to demonstrate that the Vip1/Vip2 binary toxin is active against these Scarabaeoidea larvae. The cry8-like gene appeared to be a C-terminally truncated form of a typical cry8 gene and was not expressed in our usual recombinant Bt expression system. When however the missing C-terminal region was replaced with the corresponding sequence from cry8Ea, the resulting hybrid expressed well and the toxin was active against the three test insects. © 2014, Springer-Verlag Berlin Heidelberg.


Zhang F.,Heilongjiang Bayi Agricultural University | Zhang F.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | Crickmore N.,University of Sussex | And 5 more authors.
Applied and Environmental Microbiology | Year: 2016

With the aim of optimizing the cloning of novel genes from a genomic pool containing many previously identified homologous genes, we designed a redundant exclusion PCR (RE-PCR) technique. In RE-PCR, a pair of generic amplification primers are combined with additional primers that are designed to specifically bind to redundant, unwanted genes that are a subset of those copied by the amplification primers. During RE-PCR, the specific primer blocks amplification of the full-length redundant gene. Using this method, we managed to clone a number of cry8 or cry9 toxin genes from a pool of Bacillus thuringiensis genomic DNA while excluding amplicons for cry9Da, cry9Ea, and cry9Eb. The method proved to be very efficient at increasing the number of rare genes in the resulting library. One such rare (and novel) cry8-like gene was expressed, and the encoded toxin was shown to be toxic to Anomala corpulenta. © 2016, American Society for Microbiology. All Rights Reserved.


PubMed | Heilongjiang Bayi Agricultural University, University of Sussex, Chinese Academy of Agricultural Sciences and Cangzhou Academy of Agricultural and Forestry science
Type: Journal Article | Journal: Applied and environmental microbiology | Year: 2016

With the aim of optimizing the cloning of novel genes from a genomic pool containing many previously identified homologous genes, we designed a redundant exclusion PCR (RE-PCR) technique. In RE-PCR, a pair of generic amplification primers are combined with additional primers that are designed to specifically bind to redundant, unwanted genes that are a subset of those copied by the amplification primers. During RE-PCR, the specific primer blocks amplification of the full-length redundant gene. Using this method, we managed to clone a number of cry8 or cry9 toxin genes from a pool of Bacillus thuringiensis genomic DNA while excluding amplicons for cry9Da, cry9Ea, and cry9Eb The method proved to be very efficient at increasing the number of rare genes in the resulting library. One such rare (and novel) cry8-like gene was expressed, and the encoded toxin was shown to be toxic to Anomala corpulentaProtein toxins from the bacterium Bacillus thuringiensis are being increasingly used as biopesticides against a wide range of insect pests, yet the search for new or improved toxins is becoming more difficult, as traditional methods for gene discovery routinely isolate previously identified clones. This paper describes an approach that we have developed to increase the success rate for novel toxin gene identification through reducing or eliminating the cloning of previously characterized genes.


Shu C.,Chinese Academy of Agricultural Sciences | Tan S.,Chinese Academy of Agricultural Sciences | Yin J.,Chinese Academy of Agricultural Sciences | Soberon M.,National Autonomous University of Mexico | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2015

Holotrichia parallela is one of the most severe crop pests in China, affecting peanut, soybean, and sweet potato crops. Previous work showed that Cry8Ea toxin is highly effective against this insect. In order to identify Cry8Ea-binding proteins in the midgut cells of H. parallela larvae, we assembled a midgut tissue transcriptome by high-throughput sequencing and used this assembled transcriptome to identify Cry8Ea-binding proteins by liquid chromatography-tandem mass spectrometry (LC-MS/MS). First, we obtained de novo sequences of cDNAs from midgut tissue of H. parallela larvae and used available cDNA data in the GenBank. In a parallel assay, we obtained 11 Cry8Ea-binding proteins by pull-down assays performed with midgut brush border membrane vesicles. Peptide sequences from these proteins were matched to the H. parallela newly assembled midgut transcriptome, and 10 proteins were identified. Some of the proteins were shown to be intracellular proteins forming part of the cell cytoskeleton and/or vesicle transport such as actin, myosin, clathrin, dynein, and tubulin among others. In addition, an apolipophorin, which is a protein involved in lipid metabolism, and a novel membrane-bound alanyl aminopeptidase were identified. Our results suggest that Cry8Ea-binding proteins could be different from those characterized for Cry1A toxins in lepidopteran insects. © 2015, Springer-Verlag Berlin Heidelberg.


Tian B.,Cangzhou Academy of Agricultural and Forestry science | Wang J.,Cangzhou Academy of Agricultural and Forestry science | Zhang L.,Cangzhou Academy of Agricultural and Forestry science | Li Y.,Cangzhou Academy of Agricultural and Forestry science | And 2 more authors.
Euphytica | Year: 2010

Lodging results in serious yield and quality reductions in foxtail millet (Setaria italica). Among summer crops, foxtail millet is a high density and soft stalked crop that is prone to lodging. Field selection for lodging resistance is difficult because the occurrence of lodging depends on the environmental conditions. A practical method for the evaluation of lodging is not available for foxtail millet, so an understanding of lodging-associated traits and identifying a suitable method to assess lodging are essential to select for lodging resistance and to predict the risk of lodging in a cultivar. In the present study, six stem and root traits associated with lodging was measured in the field and lodging coefficients were determined to evaluate lodging resistance in landrace and improved cultivars. Based on the results of correlation analysis, lodging coefficient is a suitable indicator for field selection for lodging resistance in both classes of cultivars tested for breeding purpose. Statistical analyses demonstrated that lodging was associated with stem quality rather than plant height and internode length at the stem base. The most important trait that directly impacted on lodging coefficient was mechanical strength of the stem and plant height in the landraces, while the weights of the above- and under-ground tissues, in combination with mechanical strength of the stem, were the most important contributors to lodging coefficient in the improved cultivars. © 2009 Springer Science+Business Media B.V.


Liu J.,Chinese Academy of Agricultural Sciences | Yan G.,Chinese Academy of Agricultural Sciences | Shu C.,Chinese Academy of Agricultural Sciences | Zhao C.,Chinese Academy of Agricultural Sciences | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2010

A newly engineered strain, denominated BIOT185, was constructed through integrating the cry8Ca2 gene into the endogenous plasmid of BT185 (contains cry8Ea1) by homologous recombination. The thermosensitive plasmid vector was eliminated by the rising temperature of recombinant cultures. No antibiotic gene or other unnecessary genes were introduced to the new strain. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis demonstrated that the cry8Ca2 gene was expressed normally and produced a 130-kDa protein in the BIOT185 strain. Bioassay results showed that the new strain had high toxicity to the pests Anomala corpulenta and Holotrichia parallela, which often damage the same fields. © 2010 Springer-Verlag.


Tian B.,Cangzhou Academy of Agricultural and Forestry science | Zhang L.,Cangzhou Academy of Agricultural and Forestry science | Liu Y.,Cangzhou Academy of Agricultural and Forestry science
Journal of Plant Registrations | Year: 2016

Summer foxtail millet [Setaria italica (L.) Beauv.] is widely grown in the northern part of China, especially in Hebei Province, where it accounts for one-fourth of the country’s producing areas.A winter wheat (Triticum aestivum L.)– summer foxtail millet–winter wheat rotation is a typical practice, so early maturity of foxtail millet is critical in this system.The objective of this research was to develop a summer foxtail millet cultivar with early maturity and promising agronomical performance.‘Canggu 5’ (Reg.No.CV-1, PI 676976) summer foxtail millet was developed from the cross Ji 8787/Shui 2, which was made in 1999 and released in December 2013 by Cangzhou Academy of Agricultural and Forestry Sciences (Certificate no.2013001).Canggu 5 was assessed in multi-environment replicated plots in the Northern China Summer Foxtail Millet Regional trials (NCSFMRT) from 2011 to 2013.Canggu 5 was 3 d earlier in maturity than the control cultivar Jigu 19.Canggu 5 was moderately resistant to stem lodging, which was similar to Jigu 19.Grain yields were consistently better than Jigu 19.The end-use quality was equal or better than Jigu 19.Canggu 5 is an early maturing cultivar and is agronomically competitive in the summer foxtail millet production regions in northern China. © Crop Science Society of America.

Loading Cangzhou Academy of Agricultural and Forestry science collaborators
Loading Cangzhou Academy of Agricultural and Forestry science collaborators