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Wu Y.-L.,Guangdong General Hospital and Guangdong Academy of Medical science | Lu S.,Shanghai JiaoTong University | Cheng Y.,Cancer Hospital of Jilin Province | Zhou C.,Tongji University | And 11 more authors.
Lung Cancer | Year: 2014

Objectives: Retrospective subgroup analysis in JMDB study indicates that the between-arm differences in overall survival (OS) in the East Asian subgroup were consistent with those observed in the entire JMDB study population. This bridging study (JMIL) further evaluated the efficacy and safety of first-line pemetrexed/cisplatin (PC) versus gemcitabine/cisplatin (GC) in Chinese patients with nonsquamous non-small cell lung cancer (NSCLC). The primary endpoint of this local registration trial was designed to compare OS in the combined dataset, consisting of Chinese patients in JMIL and 1252 nonsquamous patients in JMDB. Materials and methods: Chinese patients with stage IIIB/IV nonsquamous NSCLC were randomly assigned (1:1) to 6 cycles maximum (21 days/cycle) of pemetrexed 500mg/m2+cisplatin 75mg/m2 (day 1), or gemcitabine 1250mg/m2 (days 1 and 8)+cisplatin 75mg/m2 (day 1). Results: In JMIL, 256 Chinese patients were randomized (PC, n = 126; GC, n = 130). Patient baseline characteristics were balanced between treatment arms. In the combined dataset, PC was superior to GC in prolonging OS, with adjusted hazard ratio (HR) of 0.87 (95% CI: 0.77-0.98, p = 0.023) and median OS of 11.76 versus 10.94 months. In the JMIL-only population, no significant OS difference observed between treatment arms (adjusted HR. = 1.03 [95% CI: 0.77-1.39, p = 0.822]; unadjusted HR. = 0.996 [95% CI: 0.74-1.33, p = 0.980]), nor for other secondary efficacy endpoints. Significantly fewer patients in the PC arm experienced drug-related grade 3/4 toxicities, 54 (43.2%) versus 71 (55.9%) for GC (p = 0.045), with significantly lower rates of leukocytopenia, thrombocytopenia, and fatigue. Conclusion: This study showed that in the combined population, OS of PC was superior to GC, while in the Chinese-only population, no significant difference was observed; a better safety and risk/benefit profile was found in the PC arm. A PC regimen should be considered as a standard of care in Chinese nonsquamous NSCLC patients in a first-line setting. © 2014 Elsevier Ireland Ltd. Source


Zhang P.,Jilin University | He Y.,Jilin University | Liu J.,Jilin University | Feng J.,CAS Changchun Institute of Applied Chemistry | And 4 more authors.
RSC Advances | Year: 2016

Lanthanide-doped nanocrystals have been researched extensively and used for bioimaging because of their optical properties, magnetic properties and X-ray absorption. Core-shell-structured lanthanide-doped nanocrystals have been developed and characterized by TEM and XRD analysis. The nanocrystals are composed of BaYbF5:0.5% Tm as the core and BaGdF5:20% Yb, 0.5% Tm as the shell. Apart from characterization of the nanocrystals, evaluation of both their cytotoxicity via MTT assays and their long-term toxicity via histological analysis showed that their cytotoxicity was low, indicating the possibility of further in vivo imaging. This work combined the functions of trimodal imaging into one nanoplatform and then UCL, CT, and MR imaging with core-shell-structured nanocrystals were investigated both in vitro and in vivo. Taking into consideration its structural characteristics and trimodal imaging abilities, it is expected that the developed multifunctional nanoplatform may be potentially useful for diagnosing diseases at an early stage. © 2016 The Royal Society of Chemistry. Source


Liu X.,Jilin University | Yan S.,Cancer Hospital of Jilin Province | Pei C.,Jilin Province Peoples Hospital | Cui Y.,Jilin University
Molecular Medicine Reports | Year: 2015

MicroRNA-132 (miR-132) has been shown to be dysregulated in certain types of human malignancies and is associated with tumor progression. However, its function in non-small cell lung cancer (NSCLC) and whether it is differentially expressed in this disease, remain unclear. Thus, the aim of the present study was to investigate the effects of miR-132 on NSCLC tumorigenesis and progression. Using reverse transcription-quantitative polymerase chain reaction, miR-132 expression was detected in NSCLC cell lines and primary tumor tissues. The association between miR-132 expression, and clinicopathological factors and prognosis was assessed using statistical analysis. An MTT assay, flow cytometry, Transwell invasion assays and scratch migration assays were conducted in order to examine the proliferation, apoptosis, invasion and migration of NSCLC cells that had been transfected with miR-132 mimics or inhibitors. The results showed that miR-132 expression levels were significantly downregulated in NSCLC cells compared with that in corresponding non-cancerous lung tissues (P<0.001). In addition, reduced miR-132 expression was significantly associated with lymph node metastasis (P=0.003), an advanced tumor-node-metastasis stage (P<0.001) and shorter overall survival (P<0.001). Multivariate regression analysis confirmed that downregulation of miR-132 was an independent predictor of prognosis. Furthermore, transfection of miR-132 mimics into the NSCLC cells reduced cell proliferation, invasion and migration, and promoted cell apoptosis. These findings indicate that miR-132 may be a novel diagnostic and prognostic marker, as well as a potential target for molecular therapy in NSCLC. Source


Ding Y.,Jilin University | Zhang H.,Jilin University | Li Y.,Jilin University | Wu D.,Cancer Hospital of Jilin Province | And 4 more authors.
International Journal of Medical Sciences | Year: 2013

siRNA (small interfering RNA) interference represents an exciting new technology that could have therapeutic applications for the treatment of viral infections. However, a major challenge in the use of siRNA as a therapeutic agent is the development of a suitable delivery system. We demonstrated that a new non-viral transgene carrier, recombinant archaeal histone from the hyperthermophile Pyrococcus horikoshii OT3 (HPhA), can transfect short hairpin RNA (shRNA) expressing plasmids into HL-7702 cells to inhibit the expression of HCV 5'NTR and Core protein and mRNA. Plasmids Psilencirle transfected by HPhA inhibited the expression of HCV 5'-NTR and Core protein and mRNA in HL-7702 cells. The transfection efficiency of HPhA in HL-7702 cells was not affected by 10% fetal calf serum (FCS). HPhA exhibited effects of transfection without apparent toxicity, and with high affinity for DNA. This suggests that HPhA may be useful for RNAi-based gene therapy in vivo. © Ivyspring International Publisher. Source


Wang Z.,CAS Changchun Institute of Applied Chemistry | Wang Z.,University of Chinese Academy of Sciences | Feng J.,CAS Changchun Institute of Applied Chemistry | Song S.,CAS Changchun Institute of Applied Chemistry | And 6 more authors.
Journal of Materials Chemistry C | Year: 2014

The pure and intense orange upconversion luminescence of Eu3+ was efficiently achieved in NaYF4 and NaLuF4 nanocrystals through the upconversion sensitization of doped Yb3+ and Mn2+ ions dimer for the first time. A shell-coating strategy was performed to further enhance the emission intensity. In this work, a novel upconversion mechanism for energy transfer from the Yb3+-Mn2+ dimer to the Eu3+ ion was proposed by analyzing the upconversion luminescence spectra, lifetimes and pumping photons measurements of the obtained nanocrystals in detail. The applications of the nanocrystals for CT imaging and ratiometric pH sensing in physiological pH range were performed. This study also provides a new upconversion spectral region at around 592 nm for applications in multicolor imaging, multiplexed encoding and detection. © The Royal Society of Chemistry 2014. Source

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