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PubMed | Fudan University and Cancer Hospital of Baotou City
Type: Journal Article | Journal: Journal of experimental & clinical cancer research : CR | Year: 2017

Hepatocellular carcinoma (HCC) develops in a complex microenvironment characterized by chronic inflammation. In recent years, cholesterol metabolic abnormalities have been implicated the importance in cancer cell physiology. This study was designed to investigate the relationship between inflammation and cholesterol accumulation in HCC cells.Human HCC cells HepG2 and Huh7 were cultured and stimulated with lipopolysaccharide (LPS) for 24h. The changes of HCC cells related to cholesterol metabolism including intracellular cholesterol concentrations, cholesterol uptake, and the expression of cholesterol-related genes 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), LDL receptor (LDLR), sterol regulatory element-binding transcription factor 2 (SREBF2), and proprotein convertase subtilisin/kexin 9 (PCSK9) were comparatively analyzed. Simultaneously, the effects of nuclear factor-kappa B (NF-B) signaling pathway on cholesterol metabolism were clarified by knocking-down of nuclear factor kappa-B kinase subunit alpha (IKK) and TGF-beta-activated kinase 1 and MAP3K7-binding protein 3 (TAB3) via RNAi and microRNA (miR)-195. Subsequently, the roles of cholesterol accumulation in LPS induced pro-inflammatory effects were further investigated.Pro-inflammatory factor LPS significantly increased intracellular cholesterol accumulation by upregulating the expression of HMGCR, LDLR, and SREBF2, while downregulating the expression of PCSK9. These effects were revealed to depend on NF-B signaling pathway by knocking-down and overexpression of IKK and TAB3. Additionally, miR-195, a regulator directly targeting IKK and TAB3, blocked the effects of cholesterol accumulation, further supporting the critical role of pro-inflammation NF-B signaling in regulating cholesterol accumulation. Intriguingly, the accumulation of cholesterol conversely exerted an augmented pro-inflammation effects by further activating NF-B signaling pathway.These results indicated that pro-inflammation effects of NF-B signaling could be augmented by a positive feedback via enhancing the cholesterol accumulation in liver cancer cells.

Wang Y.-L.,Cancer Hospital of Baotou City | Meng L.-R.,Cancer Hospital of Baotou City | Yu H.-X.,Cancer Hospital of Baotou City
Journal of Leukemia and Lymphoma | Year: 2010

Objective: To determine the curative effect and adverse effect of thalidomide regimen in treatment of invasive non-Hodgkin lymphoma (NHL). Methods: Fifty-two patients with invasive NHL were divided into two groups randomly, with twenty-six patients in each group. In CHOP regimen (cyclophosphamide 600 mg/m2 iv d1, epirubicin 50 mg/m2 iv d1, vincristine 1.4 mg/m2 iv d1, prednisone 50 mg/m2 d1-d7) were given. In thalidomide regimen, the same shema with the CHOP regimen exception thalidomide, thalidomide 200 mg/m2 po d1-d14 in the first course, 400 mg/m2 po d1-d14 in the second time of therapy were given. Results: The response rate (RR) of CHOP regimen and thalidomide regimen were 73.1 % and 88.4 %, respectively. There was no significant difference (P >0.05). the OS rate in the first year and the second year of CHOP regimen and thalidomide regimen were 90.8 % and 68.8 %, 92.6 % and 83.5 %, respectively, There was no significant difference in the first year of OS (P >0.05). There was significant difference in the second year of OS (P <0.05). The major side effect of the Two groups had significant difference (P <0.05). Conclusion: Thalidomide combined with CHOP regimen on treatment of invasive NHL showed better efficacy than CHOP regimen on invasive NHL The toxic reactions are tolerable.

Zhu J.J.,Cancer Hospital of Baotou City | Liu X.F.,Cancer Hospital of Baotou City | Zhang P.L.,Cancer Hospital of Baotou City | Yang J.Z.,Cancer Hospital of Baotou City | And 6 more authors.
Genetics and Molecular Research | Year: 2014

This study aimed to provide additional anatomical information for axillary lymph node dissection (ALND) through in vivo anatomy studies of intercostobrachial nerve (ICBN) preservation in order to provide theoretical and practical experience for clinicians. A total of 156 patients with breast cancer underwent ALND at the Department of Gynecology of Baotou Tumor Hospital between June 2009 and March 2010. The origin, destination, main source, length, branch type, and direction of ICBN in axilla were observed, as well as its relationship with adjacent major blood vessels and nerves within the axilla. There were 120 cases of single trunk, 23 cases of double trunks, 9 cases of multiple trunks, and 4 cases without trunks in 156 patients with ICBN preservation. The transverse diameter at the origin of the ICBN was 1.89 ± 0.44 mm with a length of 94.45 ± 12.08 mm; the distances were 77.19 ± 21.04 mm, 29.34 ± 6.73 mm, 90.04 ± 13.13 mm, and 28.63 ± 13.01 mm from origin to the inferior margin at the midpoint of the clavicle, inferior margin of the axillary vein, the bottom of axilla, and branch point, respectively. The identification, dissection, and preservation of ICBN was simple and easy in a modified radical mastectomy for breast cancer and breast-conserving surgery, which only took 10-20 min, but effectively reduced the incidence of post-mastectomy pain syndrome and significantly improved the quality of life for patients after surgery. © FUNPEC-RP.

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