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Naegele R.P.,Michigan State University | Quesada-Ocampo L.M.,North Carolina State University | Kurjan J.D.,Michigan State University | Saude C.,Canadian Tobacco Research Foundation | Hausbeck M.K.,Michigan State University
Phytopathology | Year: 2016

Cucurbit downy mildew (CDM), caused by the oomycete pathogen Pseudoperonospora cubensis, is a devastating disease that affects cucurbit species worldwide. This obligate, wind-dispersed pathogen does not overwinter in Michigan or other northern regions and new isolates can enter the state throughout the growing season. To evaluate the regional and temporal population structure of P. cubensis, sporangia from CDM lesions were collected from cucurbit foliage grown in Michigan and Ontario field locations in 2011. Population structure and genetic diversity were assessed in 257 isolates using nine simple sequence repeat markers. Genetic diversity was high for isolates from Michigan and Canada (0.6627 and 0.6131, respectively). Five genetic clusters were detected and changes in population structure varied by site and sampling date within a growing season. The Michigan and Canada populations were significantly differentiated, and a unique genetic cluster was detected in Michigan. © 2016 The American Phytopathological Society.

Amankwa G.A.,Canadian Tobacco Research Foundation | Shearer A.,Canadian Tobacco Research Foundation | van Hooren D.,Canadian Tobacco Research Foundation
Journal of Crop Improvement | Year: 2012

Pearl millet (Pennisetum glaucum L.), a relatively new crop in Canada, can potentially provide major economic benefits through cover cropping. This study examined the effect of nitrogen (N) rate and biomass management on dry matter and N uptake of pearl millet in a rotation involving flue-cured tobacco (Nicotiana tabacum L.). In June 2005 and 2007, pearl millet (var. CFPM 101) was planted under three N rates (60, 120, and 180 kg ha -1) using two systems of aboveground biomass management: single cut with biomass removed from the field (BS1) or two cuts with biomass retained in the field (BS2). In addition, winter rye was established and managed with current practices for winter rye in rotation with flue-cured tobacco. Millet biomass increased up to the highest N rate in BS2, but in BS1 biomass values at the 120 and 180 kg N ha -1 rates were not statistically different (P ≤ 0.05). Depending on treatment, millet biomass was higher than the winter rye biomass by about 60% to 214% in 2005 and 20% to 145% in 2007. In the fall when millet was grown, nitrate-N concentrations in the top 20-cm soil samples were higher in BS1 than in BS2. Results indicated that different harvesting methods required different rates of N fertilization. Therefore, more research to develop N recommendations for the different production options is needed. © 2012 Copyright Taylor and Francis Group, LLC.

Amankwa G.A.,Canadian Tobacco Research Foundation | Mishra S.,Canadian Tobacco Research Foundation | Shearer A.D.,Canadian Tobacco Research Foundation | Brammall R.,Canadian Tobacco Research Foundation | van Hooren D.L.,Canadian Tobacco Research Foundation
Canadian Journal of Plant Science | Year: 2014

CTH8 is a new flue-cured tobacco (Nicotiana tabacum L.) hybrid recommended for commercial release in Canada. It has superior grade index compared to the check varieties Delgold and CT157, and its yield potential is intermediate between the two checks.

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