Campus Universitario Of Cantoblanco

Madrid, Spain

Campus Universitario Of Cantoblanco

Madrid, Spain
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Ludwig R.,Friedrich - Schiller University of Jena | Teran F.J.,Campus Universitario Of Cantoblanco | Teran F.J.,CSIC - National Center for Biotechnology | Teichgraeber U.,Friedrich - Schiller University of Jena | Hilger I.,Friedrich - Schiller University of Jena
International Journal of Nanomedicine | Year: 2017

So far, the therapeutic outcome of hyperthermia has shown heterogeneous responses depending on how thermal stress is applied. We studied whether extrinsic heating (EH, hot air) and intrinsic heating (magnetic heating [MH] mediated by nanoparticles) induce distinct effects on pancreatic cancer cells (PANC-1 and BxPC-3 cells). The impact of MH (100 μg magnetic nanoparticles [MNP]/mL; H=23.9 kA/m; f=410 kHz) was always superior to that of EH. The thermal effects were confirmed by the following observations: 1) decreased number of vital cells, 2) altered expression of pro-caspases, and 3) production of reactive oxygen species, and 4) altered mRNA expression of Ki-67, TOP2A, and TPX2. The MH treatment of tumor xenografts significantly (P#0.05) reduced tumor volumes. This means that different therapeutic outcomes of hyperthermia are related to the different responses cells exert to thermal stress. In particular, intratumoral MH is a valuable tool for the treatment of pancreatic cancers. © 2017 Ludwig et al.


PubMed | Federal University of Pampa and Campus Universitario Of Cantoblanco
Type: Journal Article | Journal: Neurotoxicity research | Year: 2016

Aluminum (Al) is a significant environmental contaminant. While a good deal of research has been conducted on the acute neurotoxic effects of Al, little is known about the effects of longer-term exposure at human dietary Al levels. Therefore, the purpose of this study was to investigate the effects of 60-day Al exposure at low doses for comparison with a model of exposure known to produce neurotoxicity in rats. Three-month-old male Wistar rats were divided into two major groups: (1) low aluminum levels, and (2) a high aluminum level. Group 1 rats were treated orally by drinking water for 60days as follows: (a) control-received ultrapure drinking water; (b) aluminum at 1.5mg/kg b.w., and (c) aluminum at 8.3mg/kg b.w. Group 2 rats were treated through oral gavages for 42days as follows: (a) control-received ultrapure water; (b) aluminum at 100mg/kg b.w. We analyzed cognitive parameters, biomarkers of oxidative stress and acetylcholinesterase (AChE) activity in hippocampus and prefrontal cortex. Al treatment even at low doses promoted recognition memory impairment seen in object recognition memory testing. Moreover, Al increased hippocampal reactive oxygen species and lipid peroxidation, reduced antioxidant capacity, and decreased AChE activity. Our data demonstrate that 60-day subchronic exposure to low doses of Al from feed and added to the water, which reflect human dietary Al intake, reaches a threshold sufficient to promote memory impairment and neurotoxicity. The elevation of oxidative stress and cholinergic dysfunction highlight pathways of toxic actions for this metal.


Rice R.H.,Kansas State University | Gnecco E.,Campus Universitario Of Cantoblanco | King W.P.,University of Illinois at Urbana - Champaign | Szoszkiewicz R.,Kansas State University
Materials Chemistry and Physics | Year: 2013

We report on spiral wear patterns produced at constant angular velocity by hot tip atomic force microscopy (HT-AFM) on surfaces of two common amorphous polymers: polystyrene (PS) and polymethylmethacrylate (PMMA). Topography of these patterns is obtained with regular AFM cantilevers. Topography cross-sections taken from a center of each spiral at a given azimuthal angle Θ relate changes of surface corrugation hcorr with tangential velocity v of a thermal cantilever. Polymer wear is characterized by a power law hcorr(v) = α(v/vmax)-β, which yields a pre-factor α and an exponent β. Below the glass transition temperature Tg, α is polymer specific and β varies weakly between similar conditions and samples. Variations of β are hypothesized to reflect polymer relaxation processes, which are expected to vary only weakly between amorphous polymers. At and above Tg, α approaches initial thermal tip indentation depth within a polymer, β plummets, and a power law relation of hcorr with v diverges. These results are explained by heterogeneous wear around Tg due to a local nature of glass transition. At all studied temperatures, additional wear heterogeneities are found as due to position on the polymer and Θ. Variations of α and β with position on the polymer are found to be only marginally larger then uncertainties of the thermal tip-polymer interface temperature. Variations of α and β with Θ are found to be largely influenced by buckling of thermal cantilevers traveling in a spiral pattern© 2013 Elsevier B.V. All rights reserved.


Salas G.,Campus Universitario Of Cantoblanco | Salas G.,CSIC - Institute of Materials Science | Casado C.,Campus Universitario Of Cantoblanco | Teran F.J.,Campus Universitario Of Cantoblanco | And 4 more authors.
Journal of Materials Chemistry | Year: 2012

Uniform iron oxide magnetic nanoparticles, with sizes in the range 9-22 nm, have been synthesized by thermal decomposition of an iron oleate complex in 1-octadecene, controlling reaction parameters related to the nucleation and growth processes. After transferring to water through a ligand substitution process, nanoparticles display very good magnetic and magneto-thermal properties. The relationship between these properties and the size and size distribution of the particles is discussed. The colloidal stability of the nanoparticles dispersed in common biological buffers has also been studied. This journal is © 2012 The Royal Society of Chemistry.


Kubickova S.,ASCR Institute of Physics Prague | Niznansky D.,Charles University | Morales Herrero M.P.,CSIC - Institute of Materials Science | Salas G.,CSIC - Institute of Materials Science | And 2 more authors.
Applied Physics Letters | Year: 2014

Monodisperse maghemite nanoparticles with diameter ranging from 7 to 20 nm were examined by the In-field Mössbauer Spectroscopy (IFMS) in varying external magnetic field up to 6 T. Surprisingly, the small-sized particles (7 nm) exhibit nearly no spin canting in contrast to the larger particles with lower surface-to-volume ratio. We demonstrate that the observed phenomenon is originated by lower relative crystallinity of the larger particles with different internal structure. Hence, the persistence of the 2nd and 5th absorption lines in the IFMS cannot be unambiguously assigned to the surface spins. © 2014 AIP Publishing LLC.


PubMed | Federal University of Pampa, Reactivity, Rey Juan Carlos University and Campus Universitario Of Cantoblanco
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2017

Oxidative stress in known to contribute to the male reproductive dysfunction induced by mercury (Hg). Our study tested the hypothesis that the egg white hydrolysate (EWH), a potent antioxidant invitro, is able to prevent the effects of prolonged Hg exposure on male reproductive system in rats. For this, rats were treated for 60 days with: a) Untreated - saline solution (i.m.); b) Hydrolysate - EWH (1g/kg/day, gavage); c) Mercury - HgCl


PubMed | Federal University of Pampa, Reactivity, Rey Juan Carlos University and Campus Universitario Of Cantoblanco
Type: | Journal: Neurochemistry international | Year: 2016

The study aimed to investigate whether the Egg White Hydrolysate (EWH) is able to prevent the recognition memory disorders associated with long-term Hg exposure in rats. For this, male Wistar rats were treated for 60 days with: a) Untreated: saline solution (i.m.); b) Hydrolysate: EWH (1g/kg/day, gavage); c) Mercury: HgCl


Pineiro A.,Campus Universitario Of Cantoblanco | Barja I.,Campus Universitario Of Cantoblanco
Naturwissenschaften | Year: 2012

The chemical signals of solitary and territorial felid species are essential for their intraspecific communication. We studied the selection of plant substrates during the fecal marking behavior of the European wildcat Felis silvestris from September 2008 to June 2009 in a protected area in Northwest Spain. The aim of the study was to examine the selection of plants as signal posts with respect to their physical characteristics. We hypothesized that wildcats deposit their fecal marks on plants with physical characteristics (e.g., size, species, and visual conspicuousness) that enhance the olfactory and visual effectiveness of the signal. Our results indicate that diameter, plant group, visual conspicuousness, and interaction between the diameter and plant group influence the decision of wildcats to deposit their fecal marks on plants. The wildcats chose plants with greater diameters and greater visual conspicuousness as scent-marking posts. Moreover, the wildcats chose woody and herbaceous plants, and certain plant species were marked more frequently than expected at random. Indeed, our results indicate that the fecal marks were not randomly distributed on the plants: the wildcats chose to place their marks on plants with certain physical characteristics that maximized the detectability of the signal by intruders and potential mates, thus facilitating the spatial distribution of the species. © Springer-Verlag 2012.


Betancor L.,Campus Universitario Of Cantoblanco | Luckarift H.R.,Air Force Research Lab | Luckarift H.R.,Universal Technology Corporation
Biotechnology and Genetic Engineering Reviews | Year: 2010

The development of coimmobilized multi-enzymatic systems is increasingly driven by economic and environmental constraints that provide an impetus to develop alternatives to conventional multistep synthetic methods. As in nature, enzyme-based systems work cooperatively to direct the formation of desired products within the defined compartmentalization of a cell. In an attempt to mimic biology, coimmobilization is intended to immobilize a number of sequential or cooperating biocatalysts on the same support to impart stability and enhance reaction kinetics by optimizing catalytic turnover. There are three primary reasons for the utilization of coimmobilized enzymes: to enhance the efficiency of one of the enzymes by the in-situ generation of its substrate, to simplify a process that is conventionally carried out in several steps and/or to eliminate undesired by-products of an enzymatic reaction. As such, coimmobilization provides benefits that span numerous biotechnological applications, from biosensing of molecules to cofactor recycling and to combination of multiple biocatalysts for the synthesis of valuable products.


PubMed | Campus Universitario Of Cantoblanco
Type: | Journal: Biotechnology & genetic engineering reviews | Year: 2011

The development of coimmobilized multi-enzymatic systems is increasingly driven by economic and environmental constraints that provide an impetus to develop alternatives to conventional multistep synthetic methods. As in nature, enzyme-based systems work cooperatively to direct the formation of desired products within the defined compartmentalization of a cell. In an attempt to mimic biology, coimmobilization is intended to immobilize a number of sequential or cooperating biocatalysts on the same support to impart stability and enhance reaction kinetics by optimizing catalytic turnover. There are three primary reasons for the utilization of coimmobilized enzymes: to enhance the efficiency of one of the enzymes by the in-situ generation of its substrate, to simplify a process that is conventionally carried out in several steps and/or to eliminate undesired by-products of an enzymatic reaction. As such, coimmobilization provides benefits that span numerous biotechnological applications, from biosensing of molecules to cofactor recycling and to combination of multiple biocatalysts for the synthesis of valuable products.

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