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Charneca, Portugal

Carvalho L.C.R.,Campus de Caparica | Ribeiro D.,University of Porto | Seixas R.S.G.R.,University of Aveiro | Silva A.M.S.,University of Aveiro | And 6 more authors.
RSC Advances | Year: 2015

Non-steroidal anti-inflammatory drugs exert their pharmacological activity through inhibition of cyclooxygenase 1 and 2 (COX-1 and COX-2). Recent research suggests that a balanced inhibition of both COX-1 and COX-2 is the key to reduce the side-effects exhibited by COX inhibitors. We developed new benzimidazole-based compounds that showed a balanced COX inhibition, supported by molecular docking screening. The human whole blood assays demonstrated that the ester derivatives were potent inhibitors. Competitive saturation transfer difference (STD)-NMR experiments, in the presence of COX-2, using naproxen and diclofenac demonstrated that ester derivatives do not compete with diclofenac for the same binding site, but compete with the allosteric inhibitor naproxen. Combination of NMR spectroscopy with molecular docking has permitted us to detect a new naproxen-like inhibitor, which could be used for future drug development. © The Royal Society of Chemistry 2015.

Ribeiro A.R.,INESC Porto | Martinho I.,Biomolecular Diagnostic Laboratory | Tillak J.B.,INESC Porto | Bernacka-Wojcik I.,FCUP | And 5 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

In this paper an optofluidic chip for simultaneous determination of refractive index and acquisition of absorption or fluorescent spectra is described. The system comprises a microfluidic channel with multiple inlet/outlet for sample handling and a dual fiber optic probe, standing face to face across the channel, for the optical measurements. An FBG based Fabry Perot cavity, and a Braggmeter, allow for interferometric measurement of the refractive index while external illumination and a multimode fiber enable acquisition of the absorption/fluorescence spectra with a CCD spectrometer. Preliminary results showing the viability of simultaneous measurement are obtained from the characterization of mixed samples with distinct refractive index and dye concentrations. © 2012 SPIE.

Tillak J.B.,INESC Porto | Bernacka-Wojcik I.,Campus de Caparica | Barata D.,Campus de Caparica | Barata D.,Biomolecular Diagnostic Laboratory | And 3 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2011

This paper evaluates various strategies proposed for single cell refractometry and spectroscopy using fiber optic sensors and microfluidic chips. Details concerning design, fabrication and characterization of the chips will be addressed. Preliminary results obtained with alternative on-chip configurations using combination of fiber Bragg gratings with mirrored single mode and multimode fibers will be presented indicating the possibility of performing simultaneous assessment of cellular refractive index and absorption properties. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

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