Camel Racing Laboratory

Abu Dhabi, United Arab Emirates

Camel Racing Laboratory

Abu Dhabi, United Arab Emirates

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Wasfi I.A.,Camel Racing Laboratory | Saeed H.M.,Camel Racing Laboratory | Agha B.A.,Camel Racing Laboratory | Kamel A.M.,Camel Racing Laboratory | And 4 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50mm×2.1mm i.d., 3.5μm) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100ms. Data were acquired in full-scan mode over a mass range of 100-550Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5-200ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 95 to 107%. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolism of firocoxib in camels (Camelus dromedarus) (n=5) following intravenous (i.v.) administration of a dose of 0.1mgkg/body weight. The results obtained (mean±SD) were as follows: the terminal elimination half-life (t1/2β) was 5.75±2.26h, and total body clearance (ClT) was 354.1±82.6mL/kg/h. The volume of distribution at steady state (VSS) was 2344.4±238.7mL/kg. One metabolite of firocoxib was tentatively identified as desalkyl firocoxib (m/z 283). Firocoxib could be detected in plasma 3-5 days following i.v. administration in camels using a sensitive liquid chromatography high-resolution orbitrap mass spectrometry method. © 2014 Elsevier B.V.


Wasfi I.A.,Camel Racing Laboratory | Kamel A.M.,Camel Racing Laboratory | Saeed H.M.,Camel Racing Laboratory | Saleh N.A.A.,Camel Racing Laboratory | And 3 more authors.
Veterinary Journal | Year: 2011

Ethanol elimination was studied in camels (n=8) after a single bolus intravenous dose of 0.1g/kg bodyweight (BW). Blood samples were then collected at set intervals. Ethanol and ethyl glucuronide (EtG) in blood were analysed by validated static headspace gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods, respectively. Blood-ethanol concentration-time profiles were plotted for each camel and these were evaluated. A simple linear regression model was fitted to the selected data points and the slope of the fitted line was used to estimate the elimination rate, the distribution factor and turnover rate, which were 5.15mg/dL blood/h, 0.55L/kg and 0.028g/h/kg, respectively. Blood EtG concentration-time profiles were also plotted for each camel. The elimination half-life of EtG, estimated by linear regression (using the values obtained after ethanol was completely eliminated) was 2.18h. The theoretical initial blood concentration of EtG (C0), obtained by extrapolation to time zero was 23.4μg/dL. The results will be useful in monitoring alcohol doping in camels using either parent drug or metabolite. © 2010 Elsevier Ltd.


PubMed | Camel Racing Laboratory
Type: | Journal: Biomedical chromatography : BMC | Year: 2016

We developed and validated a high resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C18 column (50mm2.1mm i.d., 3.5m). Data was acquired in full-scan mode over a mass range of 200-400Da in negative electro spray mode at a resolution of 70,000. Linear calibration curves were obtained over the concentration ranges of 1.0-10,000ng/mL. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolites of furosemide in 6 camels (Camelus dromedarus) and we were able to advice on a withdrawal time of furosemide treatment before racing. This article is protected by copyright. All rights reserved.


PubMed | Camel Racing Laboratory
Type: Journal Article | Journal: Veterinary journal (London, England : 1997) | Year: 2011

Ethanol elimination was studied in camels (n=8) after a single bolus intravenous dose of 0.1g/kg bodyweight (BW). Blood samples were then collected at set intervals. Ethanol and ethyl glucuronide (EtG) in blood were analysed by validated static headspace gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods, respectively. Blood-ethanol concentration-time profiles were plotted for each camel and these were evaluated. A simple linear regression model was fitted to the selected data points and the slope of the fitted line was used to estimate the elimination rate, the distribution factor and turnover rate, which were 5.15 mg/dL blood/h, 0.55 L/kg and 0.028 g/h/kg, respectively. Blood EtG concentration-time profiles were also plotted for each camel. The elimination half-life of EtG, estimated by linear regression (using the values obtained after ethanol was completely eliminated) was 2.18 h. The theoretical initial blood concentration of EtG (C(0)), obtained by extrapolation to time zero was 23.4 g/dL. The results will be useful in monitoring alcohol doping in camels using either parent drug or metabolite.


PubMed | Camel Racing Laboratory and Sultan bin Zayed Al Nahyan Racing Camel Farm
Type: | Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences | Year: 2014

In this study, we developed a high-resolution liquid chromatography mass spectrometry method for the pharmacokinetic study of firocoxib followed by full method validation. Following a solid-phase extraction, the firocoxib and internal standard (celecoxib) were separated on an Agilent Zorbax ZDB C18 column (50 mm 2.1 mm i.d., 3.5 m) with a gradient elution using methanol and 0.1% aqueous formic acid. Data acquisition was performed at 25,000 resolution with the automatic gain set to 1,000,000 and the maximum injection time of 100 ms. Data were acquired in full-scan mode over a mass range of 100-550 Da in positive electrospray mode. Linear calibration curves were obtained over the concentration ranges of 0.5-200 ng/mL and no interfering peaks were detected at the retention time of firocoxib and internal standard in blank camel plasma samples. The mean extraction recoveries of firocoxib at three concentrations of 5, 25 and 75 ng/mL ranged from 92 to 104%. Coefficient of variation of intra-day and inter-day precision were both <10%. The accuracy of the method ranged from 95 to 107%. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolism of firocoxib in camels (Camelus dromedarus) (n=5) following intravenous (i.v.) administration of a dose of 0.1 mgkg/body weight. The results obtained (mean SD) were as follows: the terminal elimination half-life (t/) was 5.75 2.26 h, and total body clearance (ClT) was 354.1 82.6 mL/kg/h. The volume of distribution at steady state (VSS) was 2344.4 238.7 mL/kg. One metabolite of firocoxib was tentatively identified as desalkyl firocoxib (m/z 283). Firocoxib could be detected in plasma 3-5 days following i.v. administration in camels using a sensitive liquid chromatography high-resolution orbitrap mass spectrometry method.

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