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Taussig M.J.,Cambridge Protein Arrays Ltd
New Biotechnology | Year: 2016

The 2015 Alpbach Workshop on Affinity Proteomics, organised by the EU AFFINOMICS consortium, was the 7th workshop in this series. As in previous years, the focus of the event was the current state of affinity methods for proteome analysis, including complementarity with mass spectrometry, progress in recombinant binder production methods, alternatives to classical antibodies as affinity reagents, analysis of proteome targets, industry focus on biomarkers, and diagnostic and clinical applications. The combination of excellent science with Austrian mountain scenery and winter sports engender an atmosphere that makes this series of workshops exceptional. The articles in this Special Issue represent a cross-section of the presentations at the 2015 meeting. © 2016 Elsevier B.V. Source


Stoevesandt O.,Bioscience Technology | Taussig M.J.,Bioscience Technology | Taussig M.J.,Cambridge Protein Arrays Ltd
Expert Review of Proteomics | Year: 2012

Affinity proteomics is the field of proteome analysis based on the use of antibodies and other binding reagents as protein-specific detection probes. In this review, the particular strengths of affinity methods for determination of protein localization, functional characterization, biomarker discovery and intracellular applications, and their resulting impact in basic and clinical research are highlighted. An additional focus is on the requirements for systematic binder generation and current large-scale binder projects, including bioinformatic frameworks for epitope selection and for documentation of available binding reagents and their performance. In addition to current affinity proteomics methods and applications, including arrays of proteins, binders, lysates and tissues, approaches coupling mass spectrometry-based proteomics and affinity proteomics are reviewed. © 2012 Expert Reviews Ltd. Source


Stoevesandt O.,Bioscience Technology | Vetter M.,University of Kassel | Kastelic D.,Bioscience Technology | Kastelic D.,University of Ljubljana | And 4 more authors.
New Biotechnology | Year: 2011

We have previously described the 'DNA array to protein array' (DAPA) method for microarraying of proteins expressed by cell-free systems in situ on the array surface. In this technique, a DNA array on one slide acts as the template for generating a protein array on a second slide, mediated by a cell free lysate between the two juxtaposed slides. Here we explore the feature of the repeatability of the technology, in which the same DNA array is reused several times, and use the method to generate a microarray of 116 diverse proteins. The capabilities of DAPA technology in comparison with other protein array methods are discussed. © 2010 Elsevier B.V. Source


Schmidt R.,Bioscience Technology | Schmidt R.,Cambridge Protein Arrays Ltd | Cook E.A.,Bioscience Technology | Kastelic D.,Bioscience Technology | And 3 more authors.
Journal of Proteomics | Year: 2013

We have previously described a protein arraying process based on cell free expression from DNA template arrays (DNA Array to Protein Array, DAPA). Here, we have investigated the influence of different array support coatings (Ni-NTA, Epoxy, 3D-Epoxy and Polyethylene glycol methacrylate (PEGMA)). Their optimal combination yields an increased amount of detected protein and an optimised spot morphology on the resulting protein array compared to the previously published protocol. The specificity of protein capture was improved using a tag-specific capture antibody on a protein repellent surface coating. The conditions for protein expression were optimised to yield the maximum amount of protein or the best detection results using specific monoclonal antibodies or a scaffold binder against the expressed targets. The optimised DAPA system was able to increase by threefold the expression of a representative model protein while conserving recognition by a specific antibody. The amount of expressed protein in DAPA was comparable to those of classically spotted protein arrays. Reaction conditions can be tailored to suit the application of interest. Biological significance: DAPA represents a cost effective, easy and convenient way of producing protein arrays on demand. The reported work is expected to facilitate the application of DAPA for personalized medicine and screening purposes. This article is part of a Special Issue entitled: New Horizons and Applications for Proteomics [EuPA 2012]. © 2013 Elsevier B.V. Source


Stoevesandt O.,Cambridge Protein Arrays Ltd | Schmidt R.,Cambridge Protein Arrays Ltd | Heise C.,PolyAn GmbH | Schedler U.,PolyAn GmbH
BioSpektrum | Year: 2014

The DNA-array to protein-array technology (DAPA) allows the direct transcription and translation of a coded DNA-array to a protein array in the presence of a cell free expression system. The coupling efficiency of DNA and of the corresponding immobilized proteins is enhanced by using 3-dimensional epoxy surfaces. The production time of protein arrays is considerably reduced and the DNA template array can be reused for producing further protein arrays. © 2014 Springer-Verlag Berlin Heidelberg. Literatur:. Source

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