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Fan H.-M.,Cadre Ward | Fan H.-M.,Shanghai University | Sun X.-Y.,Shanghai University | Sun X.-Y.,Prevention and Treatment Center for Psychological Diseases | And 8 more authors.
Journal of Molecular Neuroscience | Year: 2015

Schizophrenia (SZ) is a debilitating psychotic disorder of unknown etiology, and the diagnosis is essentially based on clinical symptoms. So it is urgent to find an objective and feasible clinical diagnostic index for SZ. MicroRNA array was performed in peripheral blood mononuclear cells (PBMCs) obtained from young SZ patients and gender-, age-, and ethnicity-matched healthy controls. Then, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the top 10 microRNAs (miRNAs) with the highest fold change values in 55 SZ patients and 28 healthy controls, and 9 miRNAs demonstrate significant differences in expression levels (P < 0.01). Receiver operating characteristic (ROC) curve analysis showed that the combining area under the ROC curve (AUC) of the nine miRNAs was 0.973 (95 % confidence interval (CI): 0.945–1.000). miRNA target gene prediction and functional annotation analysis showed that there were significant enrichments in several gene ontology (GO) biological process and Kyoto encyclopedia of genes and genomes (KEGG) pathways associated with nervous system and brain functions, suggesting that the differentially expressed miRNAs may be involved in mechanism of SZ. We conclude that altered expression of miRNAs in PMBCs might be involved in young SZ pathogenesis and may serve as noninvasive biomarker for SZ diagnosis. © 2015, Springer Science+Business Media New York.


Ren X.,Chinese PLA General Hospital | Zhang Q.,Cadre Ward | Wang H.,Chinese PLA General Hospital | Man C.,Minority | And 4 more authors.
Medical Science Monitor | Year: 2015

Background: The aim of this study was to assess the role of intravenous iron supplementation in the prevention of AMS. Material/Methods: This was a randomized, double-blinded, placebo-controlled study. Forty-one (n=41) healthy Chinese low-altitude inhabitants living in Beijing, China (altitude of about 50 meters) were randomly assigned into intravenous iron supplementation (ISS group; n=21) and placebo (CON group; n=20) groups. Participants in the ISS group received iron sucrose supplement (200 mg) before flying to Lhasa, China (altitude of 4300 meters). Acute mountain sickness (AMS) severity was assessed with the Lake Louise scoring (LLS) system within 5 days after landing on the plateau (at high altitude). Routine check-ups, clinical biochemistry, and blood tests were performed before departure and 24 h after arrival. Results: A total of 38 participants completed the study (ISS group: n=19; CON group: n=19). The rate of subjects with AMS (LLS>3) was lower in the ISS group compared with the CON group, but no significant differences were obtained (P>0.05). There were no differences in patients’ baseline characteristics. The physiological indices were similar in both groups except for serum iron concentrations (19.44±10.02 vs. 85.10±26.78 μmol/L) and transfer-rin saturation rates (28.20±12.14 vs. 68.34±33.12%), which were significantly higher in the ISS group (P<0.05). Finally, heart rate was identified as a contributing factor of LLS. Conclusions: These preliminary findings suggest that intravenous iron supplementation has no significant protective effect on AMS in healthy Chinese low-altitude inhabitants. © Med Sci Monit, 2015.


Liu L.,Harbin Medical University | Song Y.,Harbin Medical University | Ma L.,Harbin Institute of Technology | Zang L.,Harbin Institute of Technology | And 3 more authors.
Lasers in Medical Science | Year: 2014

Photodynamic therapy (PDT) is considered a promising new strategy for liver cancer treatment. Three elements of PDT-optical output power, irradiation time, and photosensitizer concentration-play important roles in promoting cell death. This research aimed to characterize the effects of hematoporphyrin monomethyl ether (HMME)-based PDT on hepatocellular carcinoma cells HepG2 and thus elucidate the relationship between cell death and the three elements mentioned earlier. Furthermore, in this study, we present a parameter that represents the cumulative effects of these elements. The accumulation of HMME in HepG2 cells was observed by fluorescence microscopy. The absorption spectrum of HMME was detected using fluorescence spectral analysis. The viability of the treated cells was determined using the MTT assay, and cell apoptosis was evaluated using flow cytometry. We found that the fluorescence intensity was positively correlated with the incubation time for up to 2 h. The cell growth inhibition rate was significantly high and gradually increased with increasing concentrations of HMME or increasing light intensity, which was calculated as optical output power × irradiation time. Further analysis revealed an e-exponential decay of the cell survival rate to the product of the HMME concentration and the light intensity. We defined the product as parameter B (B = optical output power × irradiation time × HMME concentration). Similarly, the rate of cell apoptosis showed roughly e-exponential growth to parameter B. In conclusion, HMME-mediated PDT can significantly kill HepG2 cells, and the killing effect was related to the cumulative effects of the optical output power, the irradiation time, and the HMME concentration. Therefore, the newly defined parameter B, as a comprehensive physical quantity, may be of great significance for the regulation of light and photosensitizer according to patient-specific conditions in clinical practice. © 2014 Springer-Verlag London.


Wang L.,Qiqihar University | Wang L.,Northeast Petroleum University | Zhang Y.,Qiqihar University | Ding L.,Cadre Ward | And 4 more authors.
RSC Advances | Year: 2015

A series of novel gemini surfactants, namely, phenyl-1,4-bis[(carbamoylmethyl) N,N-dimethylalkyl ammonium chloride)] (a, b and c), was synthesized systematically and characterized by FTIR, 1H NMR, 13C NMR and MS. The surface and bulk properties were evaluated by surface tension, conductivity, viscosity, dynamic light scattering (DLS) and transmission electron microscopy (TEM) measurements. These surfactants have been found to have low surface tension (γCMC) values as compared to other categories of gemini cationic surfactants, and they form vesicles in solutions at low concentrations. The DLS and TEM studies showed that the aggregations of the above mentioned surfactants changed from larger globular vesicles and smaller globular micelles to network aggregates and then to globular vesicles with an increase in the surfactant concentration. It is assumed that this unusual aggregation behavior is related to the transformation of the molecular conformation of phenyl-1,4-bis(carbamoylmethyl) spacer, along with its rigidity and hydrogen-bonding capability. The thermodynamic parameters of micellization process, namely, standard Gibbs free energy (ΔG0m), enthalpy (ΔH0m) and entropy (ΔS0m), were derived from conductivity measurements at different temperatures. The Krafft points of three gemini surfactants are very low. © The Royal Society of Chemistry 2015.


Wang L.,Qiqihar University | Wang L.,Northeast Petroleum University | Qin H.,Qiqihar University | Ding L.,Cadre Ward | And 5 more authors.
Journal of Surfactants and Detergents | Year: 2014

A class of novel cationic Gemini imidazolium surfactants containing amide groups as the spacer were synthesized from ethylenediamine and 1-bromoalkane(C8, C10, C12, C14, C16) by N-alkylation to get N,N'-dialkyl ethylenediamine (1a-e), 1a-e was further reacted with chloroacetyl chloride by N-acylation to get N,N'-(ethane-1,2-diyl)bis(2-chloro-N-alkylacetamide) (2a-e), which was further reacted respectively with 1-methyl imidazole by quaternized to form the surfactant molecule, N,N'-((ethane-1,2-diyl)bis(alkyl-azanediyl)bis(2-oxoethane-2,1-diyl)) bis(1-methyl-1H-imidazol-3-ium) dichloride. The structures of intermediates (1a-e) and (2a-e) were characterized by IR and 1H NMR. The structures of the surfactants (3a-e) were characterized by IR, 1H-NMR and 13C-NMR and element analysis. The critical micelle concentrations (CMC) of 3a-e were determined by the conductivity method at 25 °C. The CMC values decreased with increasing the length of the hydrophobic chain. The surfactants (3a-e) showed good foaming stability, emulsion ability and wetting ability. The surfactants (3a-e) also have good antimicrobial activity against Staphylococcus aureus, Escherichia coli and Bacillus subtilis. © 2014 AOCS.

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