Cabdul Hakeem College

Tamilnadu, India

Cabdul Hakeem College

Tamilnadu, India
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Sathiyaraj K.,Cabdul Hakeem College | Sivaraj A.,Cabdul Hakeem College | Kumar P.V.,Cabdul Hakeem College | Devi K.,DKM College for Women | Kumar B.S.,Cabdul Hakeem College
International Journal of PharmTech Research | Year: 2010

The effect of aqueous leaf extract of Azadirachta indica (Neem) on reproduction was studied on male albino rats. The study was divided into three groups of six animals each. The first group (I) received distilled water serve as control. The second and third groups (II and III) of animals were administered the aqueous leaf extract daily at 250mg/kg body wt., and 350mg/kg body wt., respectively for a period of 30days. Significant decreases in the weights of testis, epididymes and seminal vesicle were observed. A dose related reduction in the testicular sperm count, epididymal sperm count and motility and abnormal sperm count were observed. The results showed that Azadirachta indica has effects on male rat reproduction, affecting the sexual behavior and epididymal sperm concentration.

Rajan N.,Sathyabama University | Anver Basha K.,Cabdul Hakeem College
Journal of Pharmaceutical Sciences and Research | Year: 2015

A simple, sensitive and fast analytical method for simultaneous determination of cabazitaxel and their synthetic impurities was developed and validated by using ultra performance liquid chromatography (UPLC). The separation was achieved on a Acquity UPLC BEH shield RP18 (100mm x 2.1mm, i.d., 1.7μm) at 30°C, with a mobile phase consisting of potassium dihydrogen phosphate/methanol/acetonitrile, (75/15/10, v/v/v) as mobile phase A/ and acetonitrile/water, (80/20, v/v) as mobile phase B at a flow rate of 0.5mL min-1 within 12.0min. The detection was made at a wavelength of 228nm by using photo diode array (PDA) detector. No interference peaks from excipients and relative retention time indicated the specificity of the method. The calibration curve showed correlation coefficients (r) > 0.99 calculated by linear regression and analysis of variance (ANOVA). The limit of detection (LOD) and limit of quantitation (LOQ), respectively, were 0.002 mg/mL and 0.006 mg/mL. Intraday and interday relative standard deviations (RSDs) were < 2.0 (drugs) and < 10% (degradation products) as well as the comparison between two different analysts, which were calculated by f test. © 2015, Pharmainfo Publications. All rights reserved.

Babu V.S.,Cabdul Hakeem College | Nambi K.S.N.,Cabdul Hakeem College | Chandra V.,University of Pune | Ishaq Ahmed V.P.,Cabdul Hakeem College | And 2 more authors.
Journal of Fish Diseases | Year: 2011

A new cell line, Indian Catfish Fin, derived from the fin tissue of Indian walking catfish, Clarias batrachus, was established and characterized. The cell line grew well in Leibovitz's L-15 medium supplemented with 15% foetal bovine serum (FBS) and has been subcultured more than 110 times since its initiation in 2007. The cells were able to grow at a range of temperature from 28 to 37°C with optimal growth at 28°C. The cell line predominantly consists of fibroblast-like cells. The growth rate of fin cells increased as the FBS concentration increased from 2% to 20% at 28°C with optimum growth at a concentration of 15% or 20% and poor growth at a concentration of 5%. The cells were found to be susceptible to fish nodavirus and IPNV-ab and infection was confirmed by cytopathic effect and reverse transcriptase-polymerase chain reaction. PCR amplification of mitochondrial 12S rRNA using primers specific to C. batrachus confirmed the catfish origin of the cell line. The cell line was characterized further by immunocytochemistry, transfection efficiency with pEGFP-N1 and cell cycle analysis by fluorescent-activated cell sorting. © 2011 Blackwell Publishing Ltd.

Sahul Hameed A.S.,Cabdul Hakeem College
Indian Journal of Animal Sciences | Year: 2010

Cell culture has become one of the major tools used in the life sciences today. Tissue Culture is the general term for the removal of cells, tissues, or organs from an animal or plant and their subsequent placement into an artificial environment conducive to growth. This environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semisolid medium that supplies the nutrients essential for survival and growth. The culture of whole organs or intact organ fragments with the intent of studying their continued function or development is called Organ Culture. When the cells are removed from the organ fragments prior to, or during cultivation, thus disrupting their normal relationships with neighboring cells, it is called Cell Culture. In this paper, development and application of fish cell lines are discussed.

Rajakumar G.,Cabdul Hakeem College | Abdul Rahuman A.,Cabdul Hakeem College
Acta Tropica | Year: 2011

Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of synthesized natural products for vector control have been a priority in this area. In this study, larvicidal activity of synthesized silver nanoparticles (AgNPs) utilizing aqueous extract from Eclipta prostrata, a member of the Asteraceae was investigated against fourth instar larvae of filariasis vector, Culex quinquefasciatus say and malaria vector, Anopheles subpictus Grassi (Diptera: Culicidae). The synthesized AgNPs characterized by UV-vis spectrum, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). SEM analyses of the synthesized AgNPs were clearly distinguishable measured 35-60nm in size. Larvae were exposed to varying concentrations of aqueous extract of synthesized AgNPs for 24h. The maximum efficacy was observed in crude aqueous, and synthesized AgNPs against C. quinquefasciatus (LC50=27.49 and 4.56mg/L; LC90=70.38 and 13.14mg/L), and against A. subpictus (LC50=27.85 and 5.14mg/L; LC90=71.45 and 25.68mg/L) respectively. The chi-square value were significant at p<0.05 level. These results suggest that the synthesized AgNPs have the potential to be used as an ideal eco-friendly approach for the control of the Culex tritaeniorhynchus and A. subpictus. This method is considered as a new approach to control vectors. Therefore, this study provides first report on the mosquito larvicidal activity of synthesized AgNPs against vectors. © 2011 Elsevier B.V.

Marimuthu S.,Cabdul Hakeem College | Rahuman A.A.,Cabdul Hakeem College | Rajakumar G.,Cabdul Hakeem College | Santhoshkumar T.,Cabdul Hakeem College | And 6 more authors.
Parasitology Research | Year: 2011

Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and eco-friendly reducing and capping agents. The present study was based on assessments of the antiparasitic activities to determine the efficacies of synthesized silver nanoparticles (AgNPs) using aqueous leaf extract of Mimosa pudica Gaertn (Mimosaceae) against the larvae of malaria vector, Anopheles subpictus Grassi, filariasis vector Culex quinquefasciatus Say (Diptera: Culicidae), and Rhipicephalus (Boophilus) microplus Canestrini (Acari: Ixodidae). Parasite larvae were exposed to varying concentrations of aqueous extract of M. pudica and synthesized AgNPs for 24 h. AgNPs were rapidly synthesized using the leaf extract of M. pudica and the formation of nanoparticles was observed within 6 h. The results recorded from UV-vis spectrum, Fourier transform infrared, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy support the biosynthesis and characterization of AgNPs. The maximum efficacy was observed in synthesized AgNPs against the larvae of A. subpictus, C. quinquefasciatus, and R. microplus (LC50=13.90, 11.73, and 8.98 mg/L, r 2=0.411, 0.286, and 0.479), respectively. This is the first report on antiparasitic activity of the plant extract and synthesized AgNPs. © 2010 Springer-Verlag.

Mansoor S.S.,Cabdul Hakeem College
International Journal of ChemTech Research | Year: 2010

The stability constants of mixed metal complexes in aqueous solutions for the ligand L-dopa (3,4-dihydroxy phenylalanine) and dopamine 2-(3,4-dihydroxy phenyl) ethylamine with Cu(II)-Ni(II), Cu(II)-Zn(II), and Ni(II)-Zn have been determined by computer based analysis of the pH titration data. The mixed metal species of the type MAM' have been detected with dopa and dopm. A qualitative attempt has been made on the comparison of the log β values, to study the Irving-Williams order of stabilities.

Rajakumar G.,Cabdul Hakeem College | Rahuman A.A.,Cabdul Hakeem College | Roopan S.M.,Vellore Institute of Technology | Khanna V.G.,Vellore Institute of Technology | And 4 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2012

In the present study, the biosynthesis of TiO 2 nanoparticles (TiO 2 NPs) was achieved by a novel, biodegradable and convenient procedure using Aspergillus flavus as a reducing and capping agent. Research on new, simple, rapid, eco-friendly and cheaper methods has been initiated. TiO 2 NPs were characterized by FTIR, XRD, AFM, SEM and TEM studies. The X-ray diffraction showed the presence of increased amount of TiO 2 NPs which can state by the presence of peaks at rutile peaks at 1 0 0, 0 0 2, 1 0 0 and anatase forms at 1 0 1 respectively. SEM observations revealed that synthesized TiO 2 NPs were spherical, oval in shape; individual nanoparticles as well as a few aggregate having the size of 62-74 nm. AFM shows crystallization temperature was seen on the roughness of the surface of TiO 2. The Minimum inhibitory concentration value for the synthesized TiO 2 NPs was found to be 40 μg ml -1 for Escherichia coli, which was corresponding to the value of well diffusion test. This is the first report on antimicrobial activity of fungus-mediated synthesized TiO 2 NPs, which was proved to be a good novel antibacterial material. © 2012 Elsevier B.V. All rights reserved.

Roopan S.M.,Vellore Institute of Technology | Rohit,Vellore Institute of Technology | Madhumitha G.,Vellore Institute of Technology | Rahuman A.,Cabdul Hakeem College | And 3 more authors.
Industrial Crops and Products | Year: 2013

Phyto-synthesis of transition metal nanoparticles is gaining importance due to their biocompatibility, low toxicity, green approach and environmental friendly nature. We used mesocarp layer extract of Cocos nucifera and was assessed for the synthesis of silver nanoparticles. The reduction of silver ions occurred when silver nitrate solution was treated with aqueous extract of C. nucifera coir at 60 °C. Synthesized silver nanoparticles (AgNPs) particles were confirmed by analysing the excitation of surface plasmon resonance (SPR) using UV-vis spectrophotometer at 433. nm. Further TEM analysis confirmed the range of particle size as 23 ± 2. nm. The XRD spectrum showed the characteristic Bragg peaks of 1. 1. 1, 2. 0. 0, 2. 2. 0 and 3. 1. 1 facets of the face centered cubic silver nanoparticles and confirms that these nanoparticles are crystalline in nature. Gas chromatography-mass spectrometry (GCMS) of coir extract confirms the presence of hydrocarbon such as nonacosane and heptacosane which may possibly influence the reduction process and stabilization of silver nanoparticles. This is the first account of C. nucifera coir assisted synthesis of biogenic Ag nanoparticles; the nanoparticles are notably smaller and better faceted compared with those synthesized by A. squamosa extracts previously reported. Synthesized Ag nanoparticles were effective anti-larvicidal agent's against Anopheles stephensi and C. quinquefasciatus. © 2012 Elsevier B.V.

Venkatesan C.,Cabdul Hakeem College | Vimal S.,Cabdul Hakeem College | Hameed A.S.S.,Cabdul Hakeem College
Journal of Biochemical and Molecular Toxicology | Year: 2013

Chitosan Tripolyphosphate (CS/TPP) nanoparticle is a biodegradable and nontoxic polysaccharide, used as a carrier for drug delivery. The morphology and particle-size measurements of the nanoparticles were studied by field emission scanning electron microscopy and Fourier Transform Infrared Spectroscopy (FTIR). This study aims to evaluate the impact of Russell's viper venom encapsulation on various factors and loading capacity, in addition to explore the physicochemical structure of nanoparticles. FTIR confirmed that tripolyphosphoric groups of TPP linked with ammonium groups of CS in the nanoparticles. Our results showed that CS can react with TPP to form stable cationic nanoparticles. The results also showed that encapsulation efficiency of venom at different concentrations of 20, 40, 60, 500, and 1000 μg/mL were achieved for CS/TPP nanoparticles at different concentrations of 1.5, 2, and 3 mg/mL. The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT (-3 (4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) assay. © 2013 Wiley Periodicals, Inc.

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