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Vellore, India

Sahul Hameed A.S.,Cabdul Hakeem College
Indian Journal of Animal Sciences | Year: 2010

Cell culture has become one of the major tools used in the life sciences today. Tissue Culture is the general term for the removal of cells, tissues, or organs from an animal or plant and their subsequent placement into an artificial environment conducive to growth. This environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semisolid medium that supplies the nutrients essential for survival and growth. The culture of whole organs or intact organ fragments with the intent of studying their continued function or development is called Organ Culture. When the cells are removed from the organ fragments prior to, or during cultivation, thus disrupting their normal relationships with neighboring cells, it is called Cell Culture. In this paper, development and application of fish cell lines are discussed. Source


Mansoor S.S.,Cabdul Hakeem College
International Journal of ChemTech Research | Year: 2010

The stability constants of mixed metal complexes in aqueous solutions for the ligand L-dopa (3,4-dihydroxy phenylalanine) and dopamine 2-(3,4-dihydroxy phenyl) ethylamine with Cu(II)-Ni(II), Cu(II)-Zn(II), and Ni(II)-Zn have been determined by computer based analysis of the pH titration data. The mixed metal species of the type MAM' have been detected with dopa and dopm. A qualitative attempt has been made on the comparison of the log β values, to study the Irving-Williams order of stabilities. Source


Deevika B.,Dkm College For Women Autonomous | Asha S.,Dkm College For Women Autonomous | Taju G.,Cabdul Hakeem College | Nalini T.,Dkm College For Women Autonomous
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Cadmium, found to be a toxic metal causes damage to organs such as kidney, lungs, bones, liver, brain and reproductive organ testes. But the most affected organ is kidney on cadmium exposure. In this study, cadmium toxicity and curcumin antioxidant activity against nephrotoxicity were analysed. Here twenty four rats were taken and divided into four groups,each group comprised of six animals each. The first group served as control group with a normal feed and water. The second group received cadmium acetate in distilled water(200 mg/kg of body weight) for 7 days. The third group received only curcumin, dissolved in glycerol (250 mg/kg of body weight) for the same experimental days. Finally, the fourth group is the pretreated group. Rats were treated with curcumin and cadmium acetate continuously for 7 days. At the end of experiment, animals were sacrificied and the blood sample, tissue samples analysed. The level of urea and creatinine in serum were found to be increased, the antioxidant enzymes like superoxide dismutase, catalase were decreased in tissue sample. All these, confirmed the protective activity of curcumin on induced nephrotoxicity. Source


Rajakumar G.,Cabdul Hakeem College | Abdul Rahuman A.,Cabdul Hakeem College
Acta Tropica | Year: 2011

Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of synthesized natural products for vector control have been a priority in this area. In this study, larvicidal activity of synthesized silver nanoparticles (AgNPs) utilizing aqueous extract from Eclipta prostrata, a member of the Asteraceae was investigated against fourth instar larvae of filariasis vector, Culex quinquefasciatus say and malaria vector, Anopheles subpictus Grassi (Diptera: Culicidae). The synthesized AgNPs characterized by UV-vis spectrum, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). SEM analyses of the synthesized AgNPs were clearly distinguishable measured 35-60nm in size. Larvae were exposed to varying concentrations of aqueous extract of synthesized AgNPs for 24h. The maximum efficacy was observed in crude aqueous, and synthesized AgNPs against C. quinquefasciatus (LC50=27.49 and 4.56mg/L; LC90=70.38 and 13.14mg/L), and against A. subpictus (LC50=27.85 and 5.14mg/L; LC90=71.45 and 25.68mg/L) respectively. The chi-square value were significant at p<0.05 level. These results suggest that the synthesized AgNPs have the potential to be used as an ideal eco-friendly approach for the control of the Culex tritaeniorhynchus and A. subpictus. This method is considered as a new approach to control vectors. Therefore, this study provides first report on the mosquito larvicidal activity of synthesized AgNPs against vectors. © 2011 Elsevier B.V. Source


Roopan S.M.,Vellore Institute of Technology | Rohit,Vellore Institute of Technology | Madhumitha G.,Vellore Institute of Technology | Rahuman A.,Cabdul Hakeem College | And 3 more authors.
Industrial Crops and Products | Year: 2013

Phyto-synthesis of transition metal nanoparticles is gaining importance due to their biocompatibility, low toxicity, green approach and environmental friendly nature. We used mesocarp layer extract of Cocos nucifera and was assessed for the synthesis of silver nanoparticles. The reduction of silver ions occurred when silver nitrate solution was treated with aqueous extract of C. nucifera coir at 60 °C. Synthesized silver nanoparticles (AgNPs) particles were confirmed by analysing the excitation of surface plasmon resonance (SPR) using UV-vis spectrophotometer at 433. nm. Further TEM analysis confirmed the range of particle size as 23 ± 2. nm. The XRD spectrum showed the characteristic Bragg peaks of 1. 1. 1, 2. 0. 0, 2. 2. 0 and 3. 1. 1 facets of the face centered cubic silver nanoparticles and confirms that these nanoparticles are crystalline in nature. Gas chromatography-mass spectrometry (GCMS) of coir extract confirms the presence of hydrocarbon such as nonacosane and heptacosane which may possibly influence the reduction process and stabilization of silver nanoparticles. This is the first account of C. nucifera coir assisted synthesis of biogenic Ag nanoparticles; the nanoparticles are notably smaller and better faceted compared with those synthesized by A. squamosa extracts previously reported. Synthesized Ag nanoparticles were effective anti-larvicidal agent's against Anopheles stephensi and C. quinquefasciatus. © 2012 Elsevier B.V. Source

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