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Patent
Butamax Advanced Biofuels Llc | Date: 2016-06-10

Gasoline blends and methods for producing gasoline blends containing high concentrations of a butanol isomer and having good cold start and warm-up driveability characteristics are disclosed.


Patent
Butamax Advanced Biofuels Llc | Date: 2016-06-30

The present invention relates to a catalytic process for making isooctenes using a reactant comprising isobutanol and water. The isooctenes so produced are useful for the production of fuel additives.


Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.


Patent
Butamax Advanced Biofuels Llc | Date: 2016-08-04

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased K_(M )for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.


The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.


Patent
Butamax Advanced Biofuels LLC | Date: 2016-09-29

Systems and processes for the production of fuel and fuel blends involve the production of fuels for blending with one or more alcohols such as ethanol and/or butanol. A method for producing a fuel blend includes blending a light distillate product from an oil refinery with butanol. The fuel blending can be at the oil refinery.


Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.


Patent
Butamax Advanced Biofuels Llc | Date: 2016-05-17

The invention relates to butanol compositions for fuel blending and fuel blends comprising such compositions. The compositions and fuel blends of the invention have desirable performance characteristics and can serve as alternatives to ethanol-containing fuel blends. The invention also relates to methods for producing such butanol compositions and fuel blends.


Patent
Butamax Advanced Biofuels LLC | Date: 2016-02-05

Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an FeS cluster for their activity. The yeast strains have reduced activity of an endogenous FeS protein. Activities of heterologous bacterial, fungal or plant 2Fe-2S dihydroxy-acid dehydratases and FeS propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.


Patent
Butamax Advanced Biofuels Llc | Date: 2016-03-11

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

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