Busan Regional Korea Food and Drug Administration

Busan, South Korea

Busan Regional Korea Food and Drug Administration

Busan, South Korea
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Kim K.-H.,Busan Regional Korea Food and Drug Administration | Kim K.-H.,Pukyong National University | Yu D.,University of Saskatchewan | Eom S.-H.,Korea Food Research Institute | And 5 more authors.
Journal of Applied Phycology | Year: 2017

Phlorotannins from the brown alga, Eisenia bicyclis, are known to possess strong antimicrobial activity against several microbial pathogens. In this study, the antifungal activity of phlorotannins against Candida albicans was evaluated. Among the six phlorotannins tested in the present study, fucofuroeckol-A (FF) exhibited potent antifungal activity with minimum inhibitory concentration (MIC) value of 512 μg mL−1 against C. albicans strains. It is also observed that FF restored the antifungal effect of fluconazole against fluconazole-resistant C. albicans in the combination mode. The MIC values of fluconazole against C. albicans strains drastically decreased by three- to fourfold in the combination of FF-fluconazole. Furthermore, analysis of fractional inhibitory concentration (FIC) indices revealed that the median ΣFIC against C. albicans strains ranged from 0.287 to 0.508 with FF-A and fluconazole combination indicating synergistic antifungal effects with restoring antifungal activity of fluconazole. The results obtained in this study imply that phlorotannins especially FF derived from E. bicyclis can be a potential source to develop an antifungal agent against drug-resistant C. albicans infection. © 2017 Springer Science+Business Media B.V.


Chang H.-W.,Busan Regional Korea Food and Drug Administration | Kim K.-H.,Pukyong National University
Journal of Microbiology | Year: 2011

Bacteriophage EP23 that infects Escherichia coli and Shigella sonnei was isolated and characterized. The bacteriophage morphology was similar to members of the family Siphoviridae. The 44,077 bp genome was fully sequenced using 454 pyrosequencing. Comparative genomic and phylogenetic analyses showed that EP23 was most closely related to phage SO-1, which infects Sodalis glossinidius and phage SSL-2009a, which infects engineered E. coli. Genomic comparison indicated that EP23 and SO-1 were very similar with each other in terms of gene order and amino acid similarity, even though their hosts were separated in the level of genus. EP23 and SSL-2009a displayed high amino acid similarity between their genes, but there was evidence of several recombination events in SSL-2009a. The results of the comparative genomic analyses further the understanding of the evolution and relationship between EP23 and its bacteriophage relatives. © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.


Kim E.-J.,Busan Regional Korea Food and Drug Administration | Lee H.-J.,Busan Regional Korea Food and Drug Administration | Jang J.-W.,Busan Regional Korea Food and Drug Administration | Kim I.-Y.,Busan Regional Korea Food and Drug Administration | And 8 more authors.
Korean Journal of Food Science and Technology | Year: 2010

Picrate, enzyme-picrate and instrumental analysis methods using IC (Ion Chromatography) and HPLC (High Performance Liquid Chromatography) were compared for their effectiveness in determining cyanide in extracts of Maesil, which is classified as a harmful substance. First, the picrate method showed the shortest analysis time (about 5 hr). The color of picrate paper changed at 0.01 mg/200 mL CN-. However, it was difficult to detect cyanide from amygdalin of glucosides. Second, we performed a qualitative analysis for total cyanide (free cyanide and cyanide from amygdalin) by the enzyme-picrate method using β-glucosidase and a quantitative analysis by spectrophotometry. Finally, analysis of cyanide by IC and HPLC required the longest determining time (about 17 hr) as well as pretreatment for each free cyanide and amygdalin. These results suggest that enzyme-picrate is the most effective analysis method for the detection of cyanide in Maesil extracts. © The Korean Society of Food Science and Technology.


Lee H.,Busan Regional Korea Food and Drug Administration | Lee C.,Busan Regional Korea Food and Drug Administration | Lee K.,Busan Regional Korea Food and Drug Administration | Jung Y.,Busan Regional Korea Food and Drug Administration | And 2 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2010

Sodium is a major component that maintains homeostasis and physiological state in body. It is an essential mineral that the body cannot produce for itself, so it must be supplied from food. On the other hand, overindulgence is one cause of hypertension, stroke, stomach cancer, osteoporosis and kidney disease. The purpose of this study was to monitor sodium content in meals of school foodservice. In this study, 801 samples (main/side dishes) were collected from 8 school foodservice in Busan and Gyeongsangbuk-do, Korea. Samples were classified into 21 food items, which were cooked rices, cooked rice with seasoning, noodles, cooked gruels, soups, stews, tang and chon-gol, stir-fried foods, fried foods, pan-fried foods, hard-boiled foods, roasted foods, steamed foods, seasoned vegetables, preserved foods, kimchis, sauces, fruits, breads and snacks, beverages, and dairy products. The samples were analyzed by AAS (atomic absorption spectrometry) after microwave digestion. The sodium content of sauces (1459 mg/100 g) and preserved foods (1165 mg/100 g) was higher than those of other dishes. Aside from them, sodium contents of roasted foods (894 mg/100 g), hard-boiled foods (786 mg/100 g) and kimchis (737 mg/100 g) were relatively higher than the others. Sodium intakes from meals of school foodservice were calculated by multiplying food intakes by sodium contents. The average sodium intakes from one serving of school foodservice were 605 mg for younger boys, 572 mg for younger girls, 774 mg for older boys, 730 mg for older girls in elementary school. Boys in middle school took in 1423 mg Na whereas girls consumed 1063 mg Na in middle school. Results from this study can be useful for establishing database of sodium contents and intake in meals of school foodservice. The database will be helpful for providing information on managing food for children.


Kim K.E.,Gyeongsang National University | Ko K.-H.,Gyeongnam Oriental Medicinal Herb Institute | Ko K.-H.,Gyeongnam National University of Science and Technology | Heo R.W.,Gyeongsang National University | And 7 more authors.
Journal of Medicinal Food | Year: 2016

Artemisia annua L. (AA) is a well-known source of the antimalarial drug artemisinin. AA also has an antibacterial and antioxidant activity. However, the effect of AA extract on hepatic steatosis induced by obesity is unclear. We investigated whether AA extract prevents obesity-induced insulin resistance and hepatic steatosis in high-fat diet (HFD)-fed mice. Mice were randomly divided into groups that received a normal chow diet or HFD with or without AA for 12 weeks. We found that AA extract reduced insulin resistance and hepatic steatosis in HFD-fed mice. Western blot analysis showed that HFD-induced expression of nuclear sterol regulatory element-binding protein 1 and carbohydrate-responsive element-binding protein in the livers was decreased by AA extract. In particular, dietary administration of AA extract decreased hepatic high-mobility group box 1 and cyclooxygenase-2 expression in HFD-fed mice. AA extract also attenuated HFD-induced collagen deposition and fibrosis-related transforming growth factor-β1 and connective tissue growth factor. These data indicate that dietary AA extract has beneficial effects on hepatic steatosis and inflammation in HFD-fed mice. © Kyung Eun Kim et al. 2015; Published by Mary Ann Liebert, Inc.


Kim K.-H.,Busan Regional Korea Food and Drug Administration | Kim K.-H.,Pukyong National University | Eom S.-H.,Pukyong National University | Kim H.-J.,Pukyong National University | And 5 more authors.
Fisheries and Aquatic Science | Year: 2014

With the continuing demand for new solutions in the development of effective and safe candidiasis therapies, we investigated the efficacy of an antifungal agent from the marine brown alga Eisenia bicyclis. The methanolic extract of E. bicyclis evinced potential antifungal activity against Candida species. The ethyl acetate (EtOAc)-soluble extract from E. bicyclis demonstrated the strongest antifungal activity against Candida species among five solvent-soluble extracts. Indeed, the EtOAc-soluble extract showed minimum inhibitory concentrations (MICs) ranging from 4 to 8 mg/mL. Furthermore, the EtOAc-soluble extract considerably reversed high-level fluconazole resistance of Candida species. The MIC values of fluconazole against Candida species decreased substantially (from 64 to 4 μg/mL) in combination with the MIC of the EtOAc-soluble extract (4 mg/mL). The fractional inhibitory concentration indices of fluconazole ranged from 0.531 to 0.625 in combination with 4, 2, or 1 mg/mL of the EtOAc-soluble extract against Candida isolates, indicating that these combinations exert a marked synergistic effect against Candida isolates. These findings imply that compounds derived from E. bicyclis can be a potential source of natural antifungal agents against Candida species. © 2014 The Korean Society of Fisheries and Aquatic Science.


Kim H.Y.,Gyeongnam National University of Science and Technology | Kim J.Y.,Busan Regional Korea Food and Drug Administration | Cho E.J.,Pusan National University | Choi J.M.,Pusan National University | And 9 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2015

In this study, we confirmed biological compounds from methanol (MeOH) extract of processed Polygoni Multiflori Radix (PPMR), and the radical scavenging effect and oxidative stress protective activity of MeOH extract of PPMR were investigated under in vitro conditions using LLC-PK1 renal epithelial cells. In HPLC analysis, MeOH extract of PPMR contained four species of biological compounds named 2,3,5,4'-tetrahydroxystilbene 2-O-β-D-glucoside, emodin, chrysophanol, and rhein. 2,3,5,4'-Tetrahydroxystilbene 2-O-β-D-glucoside was detected as the main compound in PPMR as 115.02 mg/kg. MeOH extract of PPMR showed 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), and hydroxyl radical scavenging activities in a concentration- dependent manner. In particular, upon 50 μg/mL of PPMR extract treatment, DPPH, ABTS, and hydroxyl radical scavenging activities were approximately 48.4%, 57.9%, and 81.2%, respectively. LLC-PK1 cell viability declined in response to oxidative stress induced by pyrogallol, sodium nitroprusside (SNP), and morpholinosydnonimine (SIN-1) generators of NO, O2-, and ONOO-, respectively. However, MeOH extract of PPMR significantly and dose-dependently inhibited oxidative-stressed LLC-PK1 cell cytotoxicity. In fact, upon 50 μg/mL of PPMR extract treatment, LLC-PK1 cell viabilities were approximately 82.1%, 89.1%, and 77.6% compared to stress levels induced by pyrogallol, SNP, and SIN-1, respectively. © 2015, Korean Society of Food Science and Nutrition. All rights reserved.


Park S.-O.,Gwangju Regional Korea Food and Drug Administration | Lee S.-H.,Busan Regional Korea Food and Drug Administration | Ahn J.-H.,Busan Regional Korea Food and Drug Administration | Jung Y.-J.,Busan Regional Korea Food and Drug Administration | And 7 more authors.
Korean Journal of Food Science and Technology | Year: 2010

In this study, a simple and rapid pre-treatment method based on liquid extraction was applied for the simultaneous determination of three macrolides (spiramycin, tylosin, and tilmicosin) residues. In these studies, the stock farm products was used as a matrix sample. When the liquid extraction method was compared with the solid phase extraction (SPE) method, the former showed higher recovery percentages and simpler steps than the latter. The macrolids were separated using a reverse-phase C18 (250 mm×4.6 mm, 5 μm) column and a gradient elution with mobile phases consisting of phosphate buffer (pH 2.5) and acetonitrile. Tylosin and tilmicosin were detected at 288 nm and spiramycin was detected at 232 nm. The average recovery percentage ranged between 83.0-90.2% for samples spiked with the three macrolids at 50 and 100 ng/g The validation results showed that the limit of detection (7 (spiramycin), 12 (tilmiconsin), 12 (tylosin) ng/g)) was under the regulatory tolerances and the linearity from calibration curves was satisfactory for determining the multi-residue of three macrolids in farm products. Monitoring samples were collected at the main cities in Korea as Seoul, Busan, Deajeon, Incheon, Deagu, and Gwangju. Microlide antibiotics were not detected in most samples. © The Korean Society of Food Science and Technology.


Kang N.S.,Busan Regional Korea Food and Drug Administration | Lee J.H.,NAKDONG River Basin Environmental Office
Food Chemistry | Year: 2011

The objective of this study was to investigate the profiles of phenolic phytochemicals in the leaves of Korean purple perilla (cv. Bora, Perilla fructescens) using reversed-phase C18 column chromatography and HPLC with DAD-ESI/MS analysis. Changes in their contents were also the first reported through eight different harvest times during two months. They were characterised as five anthocyanins and three phenolic acids including cyanidin-3,5-di-O-glucoside (1), cyanidin-3-O-glucoside (2), cyanidin-3-O-(6-O-caffeoyl)glucoside-5-O-glucoside (3), cyanidin-3-O-(6-O-coumaroyl)glucoside-5-O-glucoside (4), cyanidin-3-O-(6-O-coumaroyl)glucoside (5), caffeic acid (6), rosmarinic acid (7), and rosmarinic acid methylester (8). Significant differences were observed between individual and total phytochemical contents, especially, cyanidin-3-O-(6-O-coumaroyl)glucoside-5-O-glucoside (4) and rosmarinic acid (7) exhibited the predominant constituents. Among different harvest times, the highest content was found with 82.473mg/g on 21st September, while the lowest was 39.000mg/g on 17th August. These results may be useful in determining the optimal harvest time at which phenolic phytochemicals reaches a maximum level in mid-September. © 2010 Elsevier Ltd.


Kim H.-A.,Busan Regional Korea Food and Drug Administration | Jang J.-W.,Busan Regional Korea Food and Drug Administration | Kim D.-H.,Busan Regional Korea Food and Drug Administration | Lee H.-J.,Busan Regional Korea Food and Drug Administration | And 6 more authors.
Korean Journal of Food Science and Technology | Year: 2011

In this study, formaldehyde in various fisheries products was previously derivatized with acetylacetone and subsequently analyzed by using HPLC-PDA. The formaldehyde contents ranged from 0.07 to 73.74 mg/kg. The compound was significantly higher in both mollusks (0.34-12.38 mg/kg) and crustaceans (0.09-73.74 mg/kg) than in fish (0.07-3.35 mg/kg) and shellfish (0.50-3.90 mg/kg). This difference was due to storage time and temperature. In general, fish and shellfish are sold live or in refrigerated form with shorter a shelf-life, but mollusks and crustaceans are distributed in cold or frozen systems with a longer shelf-life. Using food intake data from a report of the National Health and Nutrition Survey, the daily human exposure level to formaldehyde was 0.58% of the ADI. The results from this study might provide fundamental information to confirm naturally-originating or fraudulent formaldehyde treatment in fisheries products. © The Korean Society of Food Science and Technology.

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