Busan Metropolitan City Institute of Health and Environment

Busan, South Korea

Busan Metropolitan City Institute of Health and Environment

Busan, South Korea
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Kim H.-T.,Busan Metropolitan City Institute of Health and Environment | Kim D.-D.,Kyungpook National University | Ku S.-K.,Daegu Haany University | Kim J.-W.,Kyungpook National University | And 3 more authors.
Journal of Veterinary Clinics | Year: 2010

Artemisia capillaries THUNB is a perennial herb that belongs to the family compositae spp. and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. The object of this study is to observe the dosage-dependent anti-obestic effects of an aqueous extracts of dried aqueous extracts of stems of Artemisia capillaris Thunberg. [Artemisiae capillaris Herba, In-Jin in Korean, INJ] on 45%/Kcal high fat diet (HFD) supplied mice. 45%/Kcal rodent HFD are supplied to ICR mice from 1 week before initiation of INJ administration throughout the 12 weeks, and after the end of 12 weeks of 62.5, 125 and 250 mg/kg/day of INJ administration, the efficacy was divided into five categories 1) hypoglycemic, 2) hepato-protective, 3) nephroprotective, 4) hypolipemic, and 5) anti- obesity effects. The effects were compared to those of simvastatin (for hypolipemic activity), silymarin (for hepatoprotective and free radical scavenger effects) and metformin (for hypoglycemic and related anti-obesity effects). 7 animals per group (8 groups; total 56 adapted mice on HFD were selected base on the body weight at 6 days after initiation of HFD supply) were used in this experiment. INJ and all three different reference drugs were directly suspended or dissolved in distilled water, and administered at a volume of 10 mL/kg, once a day for 84 days from 1 week after HFD supply. As results of 91 days of continuous HFD supply, mice showed marked obese states, hyperglycemia, hyperlipemia, liver damages and kidney damages. These mean the obesity, diabetes, diabetic hepatopathies, nephropathies and hyperlipemia were induced by HFD supply. After end of 84 days of continuous treatment of three different dosages of INJ, all diabetes related complications were inhibited; relatively favorable anti-obesity, hypolipemic, hepatoprotective, hypoglycemic and nephroprotective effects. These favorable effects showed relatively good dose-relations between all three different dosages of INJ treated, and INJ 250 mg/kg showed enough favorable effects on diabetes and related four complications tested as compared with one of each three different references. Otherwise, the efficacy of 62.5 and 125 mg/kg of INJ was somewhat slighter than those of all three reference drugs. Therefore, the suitable effective dosage of INJ is considered as 250 mg/kg/day in the present study. The overall anti-obesity effects of INJ 250 mg/kg-treated group was similar or more favorable than those of metformin 250 mg/kg-treated group, and INJ 250 mg/kg showed slighter hypoglycemic effects with silymarin 100 mg/kg and metformin 250 mg/kg, similar hypolipemic effects with simvastatin 10 mg/kg, and similar hepatoprotective effects with silymarin 100 mg/kg, and similar nephroprotective effects with that of silymarin 100 mg/kg and metformin 250 mg/kg, respectively. Obese, hyperglycemia, hyperlipemia, steatohepatitis and related nephropathies induced by HFD supply were dramatically inhibited by 84 days of continuous treatment of all three different dosages of INJ. It is, therefore expected that INJ extracts will be a favorable alternative agent for diet-related diabetes and complications.


Cho S.-J.,Pusan National University | Kim M.-H.,Busan Metropolitan City Institute of Health and Environment | Lee Y.-O.,Daegu University
Journal of Ecology and Environment | Year: 2016

Background: In order to evaluate the effect of pH, known as a critical factor for shaping the biogeographical microbial patterns in the studies by others, on the bacterial diversity, we selected two sites in a similar geographical location (site 1; north latitude 35.3, longitude 127.8, site 2; north latitude 35.2, longitude 129.2) and compared their soil bacterial diversity between them. The mountain soil at site 1 (Jiri National Park) represented naturally acidic but almost pollution free (pH 5.2) and that at site 2 was neutral but exposed to the pollutants due to the suburban location of a big city (pH 7.7). Methods: Metagenomic DNAs from soil bacteria were extracted and amplified by PCR with 27F/518R primers and pyrosequenced using Roche 454 GS FLX Titanium. Results: Bacterial phyla retrieved from the soil at site 1 were more diverse than those at site 2, and their bacterial compositions were quite different: Almost half of the phyla at site 1 were Proteobacteria (49 %), and the remaining phyla were attributed to 10 other phyla. By contrast, in the soil at site 2, four main phyla (Actinobacteria, Bacteroidetes, Proteobacteria, and Cyanobacteria) composed 94 %; the remainder was attributed to two other phyla. Furthermore, when bacterial composition was examined on the order level, only two Burkholderiales and Rhizobiales were found at both sites. So depending on pH, the bacterial community in soil at site 1 differed from that at site 2, and although the acidic soil of site 1 represented a non-optimal pH for bacterial growth, the bacterial diversity, evenness, and richness at this site were higher than those found in the neutral pH soil at site 2. Conclusions: These results and the indices regarding diversity, richness, and evenness examined in this study indicate that pH alone might not play a main role for bacterial diversity in soil. © The Author(s). 2016.


Jang E.,University of Birmingham | Jang E.,Busan Metropolitan City Institute of Health and Environment | Alam M.S.,University of Birmingham | Harrison R.M.,University of Birmingham
Atmospheric Environment | Year: 2013

Polycyclic aromatic hydrocarbons (PAH) are currently generating a great deal of interest because of their recognised toxicity, including carcinogenicity. In this study, source apportionment (SA) has been carried out using Positive Matrix Factorisation (PMF) with a dataset of 29 individual PAH (sum of vapour and particulate forms) collected by the UK National Network between 2002 and 2006. Analysis of data from 14 urban sites revealed four major source categories corresponding to unburned petroleum, diesel combustion, wood combustion and coal combustion. When a separate set of sites known to be influenced by local industrial sources was analysed, three source categories were identified corresponding to the unburned petroleum, diesel combustion and coal combustion seen in the full data analysis. When SA data were applied to the individual sites, the estimated apportionment could be explained in terms of local emission characteristics. Unburned petroleum showed the highest contribution to the sum of PAH, averaging 51.9% across the network, but benzo(a)pyrene (BaP) was more influenced by the coal combustion source which contributed 59.5% across the entire network. At the subset of sites with local industrial influence, industry was both the main contributor to the sum of PAH (accounting for 48.4% of PAH mass) and of BaP (67.9% of mass). A spatial analysis was also conducted in which the traffic source was evaluated by the difference between a roadside and a nearby urban background site, the urban source by difference between urban background and a rural site, and the industrial source by difference between a site close to a major steelworks subtracting data from a local urban background site. This showed considerable similarity between the net urban contribution and the road traffic factor, and between the net industrial contribution and the PMF coal factor profile. In both cases the congener profiles corresponded fairly well to UK national emissions inventory data. When PMF was applied separately to a more recent dataset for particle-bound PAH (2008-10) in three site groupings, it was able to distinguish the domestic coal burning source from the industry-related coal combustion source. For the urban sites, vehicle exhausts contributed the largest amount of particulate PAH and BaP across the whole year, with significant attribution to domestic coal combustion seen in the cold season. © 2013 The Authors.


Choi S.-H.,Busan Metropolitan City Institute of Health and Environment | Jeong J.-E.,Busan Metropolitan City Institute of Health and Environment | Yun N.-N.,Busan Metropolitan City Institute of Health and Environment | Kim N.-H.,Busan Metropolitan City Institute of Health and Environment | And 2 more authors.
Journal of Bacteriology and Virology | Year: 2013

We analyzed the occurrence of enteric viruses and bacteria at 22 places of drinkable groundwater (civil defense emergency water-supply facility), 8 places of the groundwater used for drinking water in group food services, and 10 places of spring-water. When the 40 concentrated samples were analyzed using nested RT-PCR and real-time RT PCR methods, norovirus and other enteric viruses were not detected in all samples tested. The detection percentages for total coliforms, Escherichia coli, Yersinia enterocolitica of fecal indicator were 57.5%, 22.5% and 7.5%, respectively. Colipages were not detected. These results suggest that high levels of fecal indicator bacteria in groundwater and spring-water are not directly related to occurrence of enteric viruses.


Lee Y.-S.,Dong - A University | Park D.-J.,Dong - A University | Park D.-J.,Busan Metropolitan City Institute of Health and Environment | Choi Y.-L.,Dong - A University
Applied Microbiology and Biotechnology | Year: 2015

A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The purified α-amylase with a molecular mass of 80 kDa, approximately, shared a sequence motif characteristic of the glycoside hydrolase family 13. The enzyme was optimally active, at 50 °C in sodium phosphate buffer (pH 6.0), by the traditional one factor-at-a-time method. But the optimal conditions of time, temperature, and pH for production of maltotriose from soluble starch were 1.76 h, 44.95 °C, and pH 6.35 by response surface methodology, respectively. Maltotriose, as the major enzyme reaction product, was analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The enzyme was found to be inhibited by the addition of 10 mM Cu2+, Fe3+, Hg2+, Zn2+, and EDTA, but exhibited extreme stability toward hexane. The Km and Vmax values for the hydrolysis of soluble starch were 1.08 mg/mL and 1.736 mmol maltotriose/mg protein/min, respectively. © 2014, Springer-Verlag Berlin Heidelberg.


Park D.-J.,Dong - A University | Park D.-J.,Busan Metropolitan City Institute of Health and Environment | Lee Y.-S.,Dong - A University | Choi Y.-L.,Dong - A University
Protein Journal | Year: 2013

Paenibacillus xylanilyticus KJ-03 isolated from konjac field, showed β-glucosidase activity on tryptic soy agar plate supplemented with 0.1 % esculin and 0.25 % ferric ammonium citrate. A genome library was constructed to obtain the β-glucosidase gene and a recombinant clone, pGlc2-3 was selected. The 2,247 bp gene encoding KJ-03 β-glucosidase consisted of 749 amino acids. The deduced amino acids of BglA were 61 % homologous with that of the β-glucosidase from Bacillus cereus AH1272, which belongs to the glycoside hydrolase family 3. His-tagged β-glucosidase was purified by using His-Trap column and characterized. KJ-03 β-glucosidase was showed as a single band with about 82 kDa on SDS-PAGE. The purified enzyme has optimal activity at 20 °C and pH 7.0 using p-NPβG and 72 % of the maximal activity was still remaining at 10 °C. The β-glucosidase has optimal activity at low temperatures indicating that it is a cold-active enzyme. The substrate specificity showed that the purified enzyme hydrolyzed aryl β-glucoside substrates and isoflavones such as daidzin and genistin. © Springer Science+Business Media New York 2013.


Jang E.,Busan Metropolitan City Institute of Health and Environment | Do W.,Busan Metropolitan City Institute of Health and Environment | Park G.,Busan Metropolitan City Institute of Health and Environment | Kim M.,Busan Metropolitan City Institute of Health and Environment | Yoo E.,Busan Metropolitan City Institute of Health and Environment
Atmospheric Pollution Research | Year: 2016

This study is based on 10-year period (2005-2014) hourly measurements of six air pollutants (NO, NO2, O3, SO2, CO and PM10) in four different sites (traffic, urban background, commercial and rural background) of Busan, the second largest city in South Korea. An integrated analysis, considering various time periods (annual, seasonal and diurnal as a function of seasons and weekdays/weekends) and a statistical analysis of concentrations in relation to local meteorological conditions has been conducted. Comparable downward trends for air pollutants over 10 years has been found across the study sites, with the exception for O3 which increases at a rate of ∼0.7 ppbv/year. NO, NO2 and CO show a seasonal variation with winter enhancement, and this pattern is most obvious at the traffic site. Ozone has an annual cycle with a spring peak, showing higher peak amplitude at the rural background site. Summer enhancement of SO2 is identified at the site in the proximity of harbours when prevailing air masses have been transported from shipping regions. The distance from sources, particularly road transportation tends to produce spatial variations in the diurnal cycle, and this is particularly evident in the broader and delayed rush hour peak at the rural background site. The meteorological conditions and source strength expressed as weekdays/weekend drive the diurnal variations in terms of peak size, showing the winter enhancement for NO, NO2, SO2 and CO with lower weekend concentrations. Highest daily peak for PM10 is observed in the spring with reduced level on weekends. © 2016 Turkish National Committee for Air Pollution Research and Control.


Jeong J.W.,Busan St Marys Medical Center | Hwang Y.H.,Busan St Marys Medical Center | Cho K.S.,Busan Metropolitan City Institute of Health and Environment | Jung M.J.,Busan Metropolitan City Institute of Health and Environment | And 3 more authors.
Korean Journal of Pediatric Infectious Diseases | Year: 2010

Purpose: The purpose of this study is to identify the viral etiology of acute respiratory illnesses and to determine epidemiology in outpatients in Busan, Korea. Methods : We collected nasal wash samples from 990 patients who visited the hospital for acute respiratory illnesses between January 2007 and December 2008. Extracted DNA or RNA from specimens was used for viral detection by an RT-PCR method. Results : Of a total of 990 samples, viruses were detected in 351 cases (35.5%). The ratio of male to female was 1.6:1 and 93.7% were less than 5 years old. Rhinovirus was detected year-round in 202 cases (57.5%), respiratory syncytial virus from October to March in 57 cases (16.2%), adenovirus year-round in 37 cases (10.5%), influenza virus from December to April in 21 cases (6%), bocavirus from January to August in 15 cases (4.3%), parainfluenza virus from April to July in 9 cases (2.6%), coronavirus from January to July in 7 cases (2%), and enterovirus from June to September in 3 cases (0.9%). Conclusion : We identified the etiology and epidemiology of viruses that caused the acute respiratory diseases that were prevalent in Busan, 2007-2008. Further surveillance will be necessary.

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