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Lian Q.-L.,China Agricultural University | Han H.-J.,China Agricultural University | Xin H.-B.,China Agricultural University | Chen L.,China Agricultural University | And 3 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2011

As a key enzyme, allene oxide synthase(AOS) plays an important role in the biosynthesis of jasmonic acid(JA). In order to study its function in the process and molecular mechanism of corm expansion for Gladiolus hybridus, a full-length cDNA of GhAOS, containing a 1533 bp open reading frame(ORF) encoding 510 putative amino acids with a calculated protein molecular weight of 56.53 kD, was cloned from the corms of G. hybridus 'Rose Supreme' through RT-PCR and RACE technologies. The tissue-specific expression analysis using RT-PCR showed that the GhAOS was expressed in leaves, stolons, flowers, roots, corms and cormels. Moreover, its RNA level was higher in leaves and stolons. In corms, the expression level of GhAOS decreased with the increasing concentrations of salicylic acid(SA) from 0, 0.1, 0.5, 1.0 to 2.0 mmol/L.In summary, results indicate that GhAOS is a novel AOS gene and repressed by SA in mRNA level, which primarily verifies the antagonistic interaction between SA and JA in signaling pathways.

Xin H.,China Agricultural University | Zhang H.,CAS Institute of Botany | Chen L.,China Agricultural University | Li X.,China Agricultural University | And 6 more authors.
Plant Cell Reports | Year: 2010

Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactivation activity and thermotolerance of transgenic Arabidopsis. In this study, the gene encoding HsfA2 with 1,053 bp open reading frame (ORF) was cloned by rapid amplification of cDNA ends (RACE) technique from Lilium longiflorum 'White heaven'. Multiple alignment and phylogenetic analyses showed that the deduced protein was a novel member of the Hsf class A2. Expression analyses by RT-PCR indicated that LlHsfA2 expression was induced by heat shock and H2O2 treatment, but not by NaCl. It was also found that the expression of LlHsfA2 correlated with thermotolerance in Lilium longiflorum 'White heaven' and Oriental hybrid 'Acapulco' under heat stress. Furthermore, yeast one-hybrid assay showed that LlHsfA2 had transactivation activity. In addition, overexpression of LlHsfA2 activated the downstream genes including Hsp101, Hsp70, Hsp25.3 and Apx2 and enhanced the thermotolerance of transgenic Arabidopsis plants. Taken together, our data suggest that LlHsfA2 is a novel and functional HsfA2, involved in heat signaling pathway in lily and useful for improvement of thermotolerance in transgenic plants. © 2010 Springer-Verlag.

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