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Martin E.,Justus Liebig University | Lodders N.,Justus Liebig University | Jackel U.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | Schumann P.,Deutsche Sammlung von Mikroorganismen und Zellkulturen | Kampfer P.,Justus Liebig University
International Journal of Systematic and Evolutionary Microbiology

A Gram-positive, non-spore-forming actinobacterium (Sj 10T) was isolated on tryptone soy agar from the air of a duck barn after filter sampling. Based on 16S rRNA gene sequence similarity studies, strain Sj 10T was shown to belong to the genus Leucobacter and was closely related to Leucobacter chromiireducens subsp. chromiireducens L-1T (97.8 %), Leucobacter tardus DSM 19811T (97.3 %) and Leucobacter luti RF6 T (97.3 %). The peptidoglycan of strain Sj 10T contained 2,4-diaminobutyric acid in combination with a lower amount of lysine as diagnostic diamino acids. In addition, threonine, glycine, alanine and glutamic acid were found. Menaquinone MK-11 was the major respiratory quinone; MK-12 and MK-10 were detected in minor amounts. The polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol and one unknown component each of a phospholipid, glycolipid and aminoglycolipid. Strain Sj 10T contained the major fatty acids anteiso-C15 : 0, iso-C 16 : 0 and anteiso-C17 : 0, like other members of the genus Leucobacter. Results of DNA-DNA hybridization, physiological and biochemical tests enabled strain Sj 10T to be differentiated genotypically and phenotypically from the most closely related Leucobacter species. Strain Sj 10T represents a novel species of the genus Leucobacter, for which the name Leucobacter aerolatus sp. nov. is proposed, with Sj 10T (=DSM 22806T =CCM 7705T) as the type strain. © 2010 IUMS. Source

Kampfer P.,Justus Liebig University | Martin E.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | Lodders N.,Justus Liebig University | Jackel U.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (Sa25T) was isolated from air of a duck barn. 16S rRNA gene and recA sequence analyses clearly placed the isolate in the vicinity of the Brucella-Ochrobactrum-Pseudochrobactrum group, with the closest relative being Pseudochrobactrum glaciei KMM 3858T. This allocation was confirmed by analyses of the quinone system (ubiquinone Q-10), fatty acid data (major fatty acids C18:1ω7c and C19:0 cyclo ω8c) and polar lipid profile (major components diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unknown aminolipid AL1; moderate amounts of three unknown polar lipids, L1-L3, an unknown aminolipid and an unknown aminophospholipid APL2). The polyamine pattern of Sa25T exhibited the major compound putrescine and moderate amounts of spermidine; a similar polyamine pattern with the major compound putrescine was also detected in Pseudochrobactrum glaciei KMM 3858T. DNA-DNA hybridization of strain Sa25T with Pseudochrobactrum glaciei KMM 3858T and the type strains of the other Pseudochrobactrum species showed values ranging from 50.3 to 24.8 %, and physiological and biochemical data clearly differentiated this isolate from the described Pseudochrobactrum species. Since Sa25T and Pseudochrobactrum glaciei KMM 3858T form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, and this separate position is supported by unique characteristics of their polyamine patterns and polar lipid profiles, we propose the novel genus Paenochrobactrum gen. nov., with the type species Paenochrobactrum gallinarii sp. nov. (type strain Sa25T =CCUG 57736T =CCM 7656T) and the reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov. (type strain Pi26T 5KMM 3858T 5NRIC 0733T 5JCM 15115T). © 2010 IUMS. Source

Kampfer P.,Justus Liebig University | Martin E.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | Lodders N.,Justus Liebig University | Langer S.,University of Veterinary Medicine Vienna | And 3 more authors.
International Journal of Systematic and Evolutionary Microbiology

A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming bacterium (Sa15T) was isolated from the air in a duck barn. On the basis of 16S rRNA gene sequence similarity studies, the organism was grouped into the class gammaproteobacteria in the neighbourhood of the genus Fulvimonas. The quinone system consisted exclusively of ubiquinone Q-8. The polar lipid profile was mainly composed of the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylcholine, and moderate amounts of phosphatidylglycerol and an unidentified lipid. This profile was substantially different from that of Fulvimonas soli LMG 19981T examined concurrently. The polyamine pattern showed the predominant amine spermidine. Major fatty acids (iso-C15 : 0, iso-C 17 : 1ω9c and iso-C17 : 0) were in agreement with its phylogenetic affiliation in the vicinity of Fulvimonas; however, differences in the polar lipid and fatty acid patterns and the polyamine profiles could be observed as well. On the basis of DNA-DNA pairing results, chemotaxonomic data and physiological and biochemical data, the strain can be clearly differentiated from Fulvimonas soli. It is evident that this organism represents a novel genus, for which the name Pseudofulvimonas gallinarii gen. nov., sp. nov. is proposed, with the type strain Sa15T (=DSM 21944T =CCM 7599T). © 2010 IUMS. Source

Spahn G.,Praxisklinik fur Unfallchirurgie und Orthopadie Eisenach | Schiele R.,Friedrich - Schiller University of Jena | Hofmann G.O.,Friedrich - Schiller University of Jena | Schiltenwolf M.,University of Heidelberg | And 6 more authors.
Physikalische Medizin Rehabilitationsmedizin Kurortmedizin

Introduction: This metaanalysis was performed to evaluate the event rate of radiological knee osteoarthritis (ER ROA) after knee injuries (minimum 10 year follow-up). Furthermore was aimed to compare the ROA after knee injury with the ROA within the normal population. Materials and methods: At deadline 28.2.2010 was performed a systematic PubMed recherch by 2 independent investigators. The search strategy was (MeSH): [knee] and [osteoarthritis] and [special injury]. Furthermore the normal ROA was evaluated for the normal population.Criteria for inclusion were papers in German or English language, minimum follow-up and clear-defined radiological osteoarthritis grading accordingly to the Kellgren-Lawrence (KL) scale. A KL-grade of 2+ was accepted as manifest radiological knee osteoarthritis.The relative risk of ROA was calculated in fixed effect model and is expressed as relative risk (odd ratio OR (CI 95%)). Results: A total of 1428 publications were found as result of the search the keywords [osteoarthritis] and [knee] and prevalence]. A total of 18 publications were included in the metaanalysis. The ROA of the normal population without adjustment to sex or age was 27.1 (CI 95% 26.627.7)%.The ROA after ACL-injury was 42.0 (CI 95% 39.844.2)%. Compared with the normal population increases an ACL-injury the ROA significantly (OR=2.5 (CI 95% 2.32.7), p<0.001). Patients after PCL-injury have a higher risk of ROA in tendency (OR=1.3 (CI 95% 0.81.9), p=0.263.Fractures near the knee joint increases the risk of ROA in tendency (OR=1.2 (CI 95% 1.01.3), p=0.05). Conclusions: Knee joint injuries are doubtless risk factors for an increased ROA. The most traumatologic outcome studies dont address the ROA. The most default of most studies is the lack of information about sex or age adjusted ROA. In future long-term follow-ups for evaluation of the age and sex adjusted ROA are needed for better understanding the progression a posttraumatic osteoarthritis. © Georg Thieme Verlag KG. Stuttgart, New York. Source

Martin E.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | Klug K.,Bundesanstalt fur Arbeitschutz und Arbeitsmedizin | Frischmann A.,University of Veterinary Medicine Vienna | Busse H.-J.,University of Veterinary Medicine Vienna | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology

Two Gram-stain-positive, non-motile, non-spore-forming cocci (strains MK-7T and MPA-33T) were isolated from poultry houses. Strain MK-7T was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. Strain MPA-33 T was isolated from the air of a turkey house on TSA after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, both strains were shown to belong to the genus Jeotgalicoccus; MK-7T was most closely related to Jeotgalicoccus psychrophilus YKJ-115T (99.3% similarity) and MPA-33T was most closely related to Jeotgalicoccus halotolerans YKJ-101T (98.8 %). The quinone system of MK-7 T was composed of equal amounts of menaquinones MK-7 and MK-6 and that of MPA-33T contained 76% MK-7 and 24% MK-6. The polar lipid profile of strain MK-7T consisted of the major compounds diphosphatidylglycerol and phosphatidylglycerol and six unidentified lipids present in minor to moderate amounts. In strain MPA-33T, diphosphatidylglycerol was the single predominant lipid, whereas phosphatidylglycerol was detected in moderate amounts. In addition, one unidentified phospholipid and four unidentified lipids were detected. Fatty acid profiles with iso-15 : 0 and anteiso-15 : 0 as major fatty acids supported the affiliation of the strains to the genus Jeotgalicoccus. The results of physiological and biochemical tests as well as DNA-DNA hybridizations allowed clear phenotypic differentiation of strains MK-7T and MPA-33 T from the most closely related species. Strains MK-7T and MPA-33T therefore represent novel species, for which the names Jeotgalicoccus coquinae sp. nov. (type strain MK-7T =DSM 22419 T =CCM 7682T =CCUG 57956T) and Jeotgalicoccus aerolatus sp. nov. (type strain MPA-33T =DSM 22420T =CCM 7679T =CCUG 57953T) are proposed. © 2011 IUMS. Source

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