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Luken D.J.,Laves Institute For Bienenkunde | Janke M.,Laves Institute For Bienenkunde | Lienau F.-W.,Laves Institute For Bienenkunde | von der Ohe W.,Laves Institute For Bienenkunde | Forster R.,Bundesamt fur Verbraucherschutz und Lebensmittelsicherheit BVL
Journal fur Verbraucherschutz und Lebensmittelsicherheit

In the LAVES Institute for Apiculture honey bee brood has been reared in the laboratory according to the method of Aupinel et al. (Bull Insectol 58:107-111, 2005) for studies on the effects of plant protection products as well as for further improvement of the method since 2008. The introduced methodological changes have a positive effect on the number of larvae available for experimental purposes. Furthermore, the treatment with MBC-solution (methyl-benzethonium chloride) could be replaced by pasteurization, which is to be viewed positively from a health perspective for the personnel but also in terms of possible synergistic effects with active ingredients. © 2012 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL). Source

Perry J.N.,Oaklands Barn | Devos Y.,GMO Unit | Arpaia S.,ENEA | Bartsch D.,Bundesamt fur Verbraucherschutz und Lebensmittelsicherheit BVL | And 10 more authors.
Proceedings of the Royal Society B: Biological Sciences

Genetically modified (GM) maize MON810 expresses a Cry1Ab insecticidal protein, derived from Bacillus thuringiensis (Bt), toxic to lepidopteran target pests such as Ostrinia nubilalis. An environmental risk to non-target Lepidoptera from this GM crop is exposure to harmful amounts of Bt-containing pollen deposited on host plants in or near MON810 fields. An 11-parameter mathematical model analysed exposure of larvae of three non-target species: The butterflies Inachis to (L.), Vanessa atalanta (L.) and moth Plutella xylostella (L.), in 11 representative maize cultivation regions in four European countries. A mortality-dose relationship was integrated with a dose-distance relationship to estimate mortality both within the maize MON810 crop and within the field margin at varying distances from the crop edge. Mortality estimates were adjusted to allow for physical effects; the lack of temporal coincidence between the susceptible larval stage concerned and the period over which maize MON810 pollen is shed; and seven further parameters concerned with maize agronomy and host-plant ecology. Sublethal effects were estimated and allowance made for aggregated pollen deposition. Estimated environmental impact was low: in all regions, the calculated mortality rate for worst-case scenarios was less than one individual in every 1572 for the butterflies and one in 392 for the moth. © 2010 The Royal Society. Source

Bernard H.,Robert Koch Institute RKI | Bisping M.,Niedersachsisches Landesamt fur Verbraucherschutz und Lebensmittelsicherheit LAVES | Broschewitz B.,Ministerium fur Landwirtschaft | Bucher M.,Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit LGL | And 20 more authors.
Journal fur Verbraucherschutz und Lebensmittelsicherheit

This is a report on the results of the work of the German EHEC Task Force on the food safety side of the EHEC O104:H4 disease outbreak investigation in Germany. During the first phase of the outbreak investigation the main goal was to identify the contaminated food. To achieve this, two different strategies were followed in parallel. One approach was a detailed trace back analysis for all salad ingredients and raw vegetables that have been served to customers at five outbreak clusters in order to identify common food sources and delivery chains. The second approach was a trace forward analysis of the supply chains of a sprout producer in order to find out if he had delivered any outbreak clusters. Both approaches revealed that contaminated sprouts from a producer in Lower Saxony highly likely had caused the outbreak. Aim of the second investigation phase was to find and stop the source of the EHEC O104:H4 bacteria. The Task Force gave recommendations on source elimination measures and collected and analysed epidemiological information in order to find out when the source was active. Next to that, a detailed trace back for batches of suspicious seeds that had been used by the sprout producer was initiated. The results from this activity formed the basis for the tracing of seeds coordinated by the European Food Safety Authority, which revealed that fenugreek seeds imported from Egypt were the most likely common link between the EHEC O104:H4 outbreaks in Germany and France. In conclusion, the newly developed outbreak investigation strategy of the task Force EHEC with close collaboration between German federal and federal state authorities and between food safety authorities, health authorities and scientists was a recipe of success and can be a model for future food-borne outbreak investigations. © 2011 Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL). Source

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