Bundaberg Research Station

Bundaberg, Australia

Bundaberg Research Station

Bundaberg, Australia

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Miyata S.,Japan National Agriculture and Food Research Organization | Kato H.,Japan National Agriculture and Food Research Organization | Davis R.,Northern Australia Quarantine Strategy | Smith M.W.,Bundaberg Research Station | And 2 more authors.
Journal of General Plant Pathology | Year: 2011

'Candidatus Liberibacter asiaticus' is the most widespread of the three species of 'Ca. Liberibacter' that cause citrus greening disease (huanglongbing). To ascertain the phylogenetic relationships among Indian isolates that have higher diversity in the 16S rDNA than Asian isolates of this species, we collected symptomatic leaves from Northeast India, Papua New Guinea and Timor-Leste (East Timor) and detected 'Ca. L. asiaticus' by PCR using primers specific for nusG-rplK genes and 16S rDNA. Phylogenetic analysis with 16S rDNA sequences and single nucleotide polymorphisms of the omp gene region revealed that the Northeast Indian isolates were genetically closer to Asian-common isolates from Japan, Taiwan, and Vietnam than to Indian isolates reported previously. Thus, the Asian-common strains of 'Ca. L. asiaticus' are apparently also present in Northeast India. © 2010 The Phytopathological Society of Japan and Springer.


Katoh H.,Japan National Agriculture and Food Research Organization | Davis R.,Northern Australia Quarantine Strategy | Smith M.W.,Bundaberg Research Station | Weinert M.,Center for Tropical Agriculture | Iwanami T.,Japan National Agriculture and Food Research Organization
Annals of Applied Biology | Year: 2012

Japanese isolates of 'Candidatus Liberibacter asiaticus' have been shown to be clearly differentiated by simple sequence repeat (SSR) profiles at four loci. In this study, 25 SSR loci, including these four loci, were selected from the whole-genome sequence and were used to differentiate non-Japanese samples of 'Ca. Liberibacter asiaticus' (13 Indian, 3 East Timorese, 1 Papuan and 8 Floridian samples). Out of the 25 SSR loci, 13 were polymorphic. Dendrogram analysis using SSR loci showed that the clusters were mostly consistent with the geographical origins of the isolates. When single nucleotide polymorphisms (SNPs) were searched around these 25 loci, only the upstream region of locus 091 exhibited polymorphism. Phylogenetic tree analysis of the SNPs in the upstream region of locus 091 showed that Floridian samples were clustered into one group as shown by dendrogram analysis using SSR loci. The differences in nucleotide sequences were not associated with differences in the citrus hosts (lime, mandarin, lemon and sour orange) from which the isolates were originally derived. © 2012 Association of Applied Biologists.


Okpul T.,University of Queensland | Maltby J.,Bundaberg Research Station | Guaf E.,Sir Alkan Tololo Research Center | Coleman E.A.,Rockhampton Research Station | And 4 more authors.
Annals of Applied Biology | Year: 2011

The accumulation and perpetuation of viral pathogens over generations of clonal propagation in crop species such as sweetpotato, Ipomoea batatas, inevitably result in a reduction in crop yield and quality. This study was conducted at Bundaberg, Australia to compare the productivity of field-derived and pathogen-tested (PT) clones of 14 sweetpotato cultivars and the yield benefits of using healthy planting materials. The field-derived clonal materials were exposed to the endemic viruses, while the PT clones were subjected to thermotherapy and meristem-tip culture to eliminate viral pathogens. The plants were indexed for viruses using nitrocellulose membrane-enzyme-linked immunosorbent assay and graft-inoculations onto Ipomoea setosa. A net benefit of 38% in storage root yield was realised from using PT materials in this study. Conversely, in a similar study previously conducted at Kerevat, Papua New Guinea (PNG), a net deficit of 36% was realised. This reinforced our finding that the response to pathogen testing was cultivar dependent and that the PNG cultivars in these studies generally exhibited increased tolerance to the endemic viruses present at the respective trial sites as manifested in their lack of response from the use of PT clones. They may be useful sources for future resistance breeding efforts. Nonetheless, the potential economic gain from using PT stocks necessitates the use of pathogen testing on virus-susceptible commercial cultivars. © 2011 Association of Applied Biologists.


Forster P.I.,Queensland Herbarium | Smith M.W.,Bundaberg Research Station
Austrobaileya | Year: 2010

A new species of Citrus is described and illustrated and its putative relationships discussed. Citrus wakonai is known from Goodenough Island, Milne Bay Archipelago in Papua New Guinea where it is a small understorey tree up to 6 m tall. It provides small edible fruit, but currently lacks potential as a source of new rootstocks or germplasm for the citrus industry due to viral susceptibility.


Smith M.W.,Bundaberg Research Station | Gultzow D.L.,Bundaberg Research Station | Newman T.K.,Bundaberg Research Station
Journal of the American Society for Horticultural Science | Year: 2013

Fruiting hybrids are reported for the first time between the genera Citrus L. and Citropsis (Engl.) Swing. & M.Kell. Conventional hybridization using the recently described species Citrus wakonai P.I.Forst. & M.W.Sm. and Citropsis gabunensis (Engl.) Swing. & M.Kell. resulted in high rates of fruit set and seed formation. Although seed were only half normal size, over 90% germinated without the need for embryo rescue techniques. Plant losses were high during the first few months but after six months, the 327 surviving hybrids were potted on. These grew vigorously on their own roots and 35 of them flowered within two years of sowing. Plants flowered continuously but all were pollen-sterile and ovaries abscised shortly after petal fall. However, at 25 months, two newly flowering hybrids began setting fruit. The development, identification, morphology, breeding efficiency, and future implications of this unique germplasm are described.

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