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Pattoki, Pakistan

Mehmood M.U.,University of Veterinary and Animal Sciences | Khan H.,University of Agriculture, Peshawar | Shahzad Q.,Buffalo Research Institute | Sattar A.,University of Veterinary and Animal Sciences
Pakistan Journal of Zoology | Year: 2014

Perosomus elumbis (Acaudatus) is an occasionally found congenital anomaly characterized by partial or complete agenesis of lumbar, sacral and coccygeal vertebrae and ankylosis of the hind limbs. A seven years old BCS 3.5±0.5 pluriparous buffalo with dystocia due to malformed fetus was presented at Theriogenology clinic. The calf had a shortened trunk and weighed roughly 32.5 kg. There was an absence of the dorsal part of the vertebrae in the fetus and misshapened sacral vertebrae with the squeezed perineal region. Small and ankylosed limbs of the fetus were loosely attached to the trunk. The monster calf was forcefully extracted with partial fetotomy result in successfully resolved dystocia of a buffalo. The malformations were mostly confined to the abdominal region. This report presents information about the successful treatment of a distance case due to perosomus elumbis (Acaudatus) reflex and the estradiol local feathering for cervical dilation yielding acceptable results. © 2014 Zoological Society of Pakistan. Source

Ahmed S.,University of Veterinary and Animal Sciences | Gohar M.,University of Veterinary and Animal Sciences | Khalique A.,University of Veterinary and Animal Sciences | Ahmad N.,University of Veterinary and Animal Sciences | And 4 more authors.
Pakistan Journal of Zoology | Year: 2016

Milk production potential of buffaloes can be exploited through fulfilling nutritional requirements in terms of protein and energy. Two essential amino acids lysine (Lys) and methionine (Met) are limiting amino acids for optimum milk production in early lactation of these animals and are derived from dietary source. The objective of the present study was to analyze the effect of rumen protected lysine and methionine on the production performance of early lactating Nili-Ravi buffaloes. In total, (n=20) lactating buffaloes with same weight and parity were divided into four groups (A, B, C and D) for 60 days trial. The groups A, B and C received basal diet+Lysine 40g + Methionine 14g, basal diet + Lysine 30g+ Methionine 10g and basal diet + 20g Lysine + 7g Methionine, respectively. Whereas group D (control group) received basal diet without lysine and methionine supplementation. Milk production, however, was significantly (P<.05) greater in groups A and B as compared with the C and D. Milk protein, total solids, solid not fat and lactose were significantly greater (P<.05) in group C than A, B and D. Blood glucose level was greater in group C as compared to all other groups. Whereas, blood urea nitrogen was significantly greater in groups A and B as compared to rest of all other groups. It can be concluded that methionine and lysine supplementation had a positive effect on milk yield and milk composition (milk protein, solid not fat, total solids and lactose). It is suggested that for the optimum production performance in early lactating Nili Ravi buffalos, lysine and methionine supplementation can be a part of their ration. Copyright 2016 Zoological Society of Pakistan. Source

Waqas M.,University of Veterinary and Animal Sciences | Mehmood M.U.,University of Veterinary and Animal Sciences | Shahzad Q.,Buffalo Research Institute | Kausar R.,Nuclear Institute for Agriculture and Biology | And 2 more authors.
Animal Reproduction Science | Year: 2016

The aim of this work was to evaluate the efficacy of G6G and Ovsynch protocols on synchronization and pregnancy rate in Nili-Ravi buffalo. For this, a total of fifty buffalo were assigned into two treatment groups: (1) G6G (n = 25) and (2) Ovsynch (n = 25). The blood samples were collected from all the treated buffalo on day (D) 7 (PGF2α injection of Ovsynch) to measure the progesterone (P4). All the buffalo were fixed time inseminated (FTAI) following last GnRH of Ovsynch. Ultrasonography was performed for follicular, corpus luteum (CL) dynamics and pregnancy diagnosis. The results revealed that synchronization rate and response to final PGF2α were significantly (P < 0.05) higher in G6G compared to Ovsynch. The ovulation to first GnRH of Ovsynch was higher (P < 0.05) in G6G (84%) than Ovsynch (56%). The mean dominant follicle size following last GnRH of Ovsynch showed significantly larger diameter in G6G (11.7 ± 0.2 mm) compared to Ovsynch (10.9 ± 0.2 mm). Moreover, the higher (P < 0.05) P4 concentration was observed in G6G than Ovsynch. The presence of double CL on D 7 was higher (P < 0.05) in G6G (64%) than Ovsynch (24%) group. The pregnancy rate (P = 0.08) tended to be greater in G6G compared to Ovsynch. In conclusion, G6G treatment showed better ovulatory and luteolytic response to first GnRH and PGF2α compared to Ovsynch treatment. Moreover, the addition of GnRH and PGF2α prior to Ovsynch is helpful to improve synchronization application of Ovsynch protocol in Nili-Ravi buffalo. © 2015 Elsevier B.V. Source

Ahmad M.,Buffalo Research Institute | Javed K.,University of Veterinary and Animal Sciences | Shahzad W.,Livestock Production Research Institute | Mehmood F.,Livestock Production Research Institute | Khan M.H.,Livestock and Dairy Development Board
Buffalo Bulletin | Year: 2013

Data on 12334 performance records of 9577 Nili-Ravi buffaloes for the parities 1 to 6 in the progeny testing program (PTP) of field areas of 10 districts i.e. Gujrat, Gujranwala, Hafizabad, Kasur, M.B. Din, Bahawalnagar, Faisalabad, Pakpattan, T.T. Singh and Vehari under Buffalo Research Institute, (BRI) Pattoki District Kasur, Punjab, Pakistan during the period 2006 to 2010 were utilized to study some environmental factors affecting the performance traits i.e. lactation length, 305-day milk yield and total lactation yield The data were analyzed by test day model using Statistical Analysis System (SAS 9.3), 2011 for fixed effects. The phenotypic correlations among various performance traits were also estimated. The least squares means for lactation length were 246.3±1.2 days. The 305-day milk yield and total lactation yield were averagely1735.3±8.1 and 1910.2±10.4 kg, respectively. The number of test days ranged from 2 to 10 for 305-day milk yield while it was 2 to 22 for total lactation yield. The phenotypic correlation among these performance traits ranged from 0.75 to 0.95. The regressions of performance traits on lactation length were also estimated. The effect of district, year and season of calving showed highly significant (P<0.01) variation for all the three performance traits while the effect of parity was highly significant (P<0.01) for 305-day milk yield and total lactation yield but non-significant for lactation length, respectively. The improvement in milk production could be achieved through efficient breeding strategy and improvement in nutrition and other management practices. Source

Shahzad Q.,Buffalo Research Institute | Imran M.,Buffalo Research Institute | Khan H.,University of Agriculture, Peshawar | Wadood A.A.,University of Veterinary and Animal Sciences | And 3 more authors.
Buffalo Bulletin | Year: 2016

Nutritional deficiency including mineral deficiency may decrease reproductive efficiency in buffaloes. Therefore, aim of the present study was to assess effect of minerals Calcium (Ca) Magnesium (Mg) and inorganic phosphorus (Pi) on cyclicity of Nili Ravi Buffaloes. The present experiment was performed at Livestock Experiment Station, Bhunikey. The female buffaloes (n=90) were divided into 3 groups; cyclic (n=30), non-cyclic (n=30) and repeat breeders (n=30). Mineral profile was measured through serum of the buffaloes under study. Calcium and Magnesium were measured through calorimetric method while inorganic phosphorus was measured through UV method. The level of calcium was significantly (P<0.05) higher in normal cyclic buffaloes than that of the non-cyclic and repeat breeders. The level of magnesium was non-significant in normal cyclic and non-cyclic buffaloes, while it was significantly (P<0.05) higher in repeat breeders as compared to cyclic and non-cyclic buffaloes. Phosphorus was not in balance in non-cyclic and repeat breeders. It is concluded from the present study that Ca: P should be 2:1. © 2016, Kasetsart University. All rights reserved. Source

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