Göttingen, Germany
Göttingen, Germany

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Larisika M.,AIT Austrian Institute of Technology | Larisika M.,Nanyang Technological University | Kotlowski C.,AIT Austrian Institute of Technology | Kotlowski C.,CEST Center Of Electrochemical Surface Technology | And 14 more authors.
Angewandte Chemie - International Edition | Year: 2015

An olfactory biosensor based on a reduced graphene oxide (rGO) field-effect transistor (FET), functionalized by the odorant-binding protein 14 (OBP14) from the honey bee (Apis mellifera) has been designed for the in situ and real-time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand-receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from Kd=4 μM to Kd=3.3 mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.


Larisika M.,Nanyang Technological University | Kotlowski C.,AIT Austrian Institute of Technology | Steininger C.,AIT Austrian Institute of Technology | Mastrogiacomo R.,University of Pisa | And 7 more authors.
Angewandte Chemie - International Edition | Year: 2015

An olfactory biosensor based on a reduced graphene oxide (rGO) field-effect transistor (FET), functionalized by the odorant-binding protein 14 (OBP14) from the honey bee (Apis mellifera) has been designed for the in situ and real-time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand-receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from Kd=4 μM to Kd=3.3 mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


PubMed | AIT Austrian Institute of Technology, University of Pisa, Buesgen Institute, Michigan State University and CEST Center Of Electrochemical Surface Technology
Type: Journal Article | Journal: Angewandte Chemie (International ed. in English) | Year: 2015

An olfactory biosensor based on a reduced graphene oxide (rGO) field-effect transistor (FET), functionalized by the odorant-binding protein14 (OBP14) from the honey bee (Apis mellifera) has been designed for the insitu and real-time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand-receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from K(d)=4M to K(d)=3.3mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein.


Zhou F.,Northwest University, China | Wang J.,Northwest University, China | Yang N.,Buesgen Institute | Zhang Q.,Northwest University, China | Zou P.,Northwest University, China
Nature Environment and Pollution Technology | Year: 2014

Through simulative methods, the influence on soil pH, the soil enzyme activities (urease, invertase and alkaline phosphatase) by the single and combined stress of water and lead were studied in a pot experiment. Five levels of Pb (0, 300, 500, 1000 and 2000 mg/kg soil) and four levels of water stress (soil relative water content (SRW), 100%, 80%, 60%, 40%) were applied. Sophora japonica and Platycladus orientalis were grown in soil-filled pots for pH and soil enzyme activities assays. The results showed that soil pH was affected by Pb and water deficiency. The addition of Pb and water stress caused soil alkalization in Platycladus orientalis, while acidification in Sophora japonica under water stress. Pb could stimulate the soil enzyme activities to some extent. The degree of influence on enzyme activities was related to plant species. Urease and invertase activities increased at 80% and 60% soil relative water content in P. orientalis and S. japonica. Interactions between Pb concentrations and water stress levels significantly impacted the three soil enzyme activities, synergism (ΔU<0) dominated the interaction in P. orientalis, while antagonism (ΔU>0) dominated the dominance for invertase and alkaline phosphatase in S. japonica.


Zhou F.,Northwest Agriculture and Forestry University | Wang J.,Northwest Agriculture and Forestry University | Yang N.,Buesgen Institute
Plant Growth Regulation | Year: 2014

The effects of lead were investigated in forest trees (Sophora japonica and Platycladus orientalis) grown in soil and exposed to Pb(CH3COO)2·3H2O (0, 300, 500, 1,000 mg kg−1) with different levels of water stress (100, 80, 60, 40 % soil relative water content, SRW). The results showed that, in the presence of Pb, the ground diameter, height and dry weight were greater than the control for S. japonica and P. orientalis. The proportions of biomass allocation of S. japonica were root > stem > leaf, and of P. orientalis leaf > stem > root except for 40 % SRW. SOD (superoxide dismutase) and POD (peroxidase) activities in leaves were activated by soil Pb for P. orientalis, while SOD activities increased but POD activities decreased compared with control under Pb stress for S. japonica. Water stress increased SOD and POD activities under different Pb concentrations, also increased MDA (malondialdehyde) content significantly. For S. japonica, the Pb concentration in the root was up to 91.9 mg kg−1, while the Pb concentration in the aboveground part was 37.5 mg kg−1. The Translocation Factor (TF value) increased with the increase of water stress intensity, under the same Pb concentration. For P. orientalis, the Pb accumulation in the roots was significantly higher than that in the aboveground part (TF < 1.0). And the total amount of Pb accumulated in P. orientalis was higher than S. japonica. On the basis of this study, it could be concluded that P. orientalis was more suitable for usage in the mining areas of northwest China to accumulate heavy metals, compared with S. japonica. © 2014, Springer Science+Business Media Dordrecht.

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