Bu Ali Research Institute

Mashhad, Iran

Bu Ali Research Institute

Mashhad, Iran

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Bideskan A.E.,Mashhad University of Medical Sciences | Sankian M.,Bu ali Research Institute | Rafatpanah H.,Mashhad University of Medical Sciences
Journal of Biomedical Science | Year: 2012

Background: Maternal epileptic seizures during pregnancy can affect the hippocampal neurons in the offspring. The polysialylated neural cell adhesion molecule (PSA-NCAM), which is expressed in the developing central nervous system, may play important roles in neuronal migration, synaptogenesis, and axonal outgrowth. This study was designed to assess the effects of kindling either with or without maternal seizures on hippocampal PSA-NCAM expression in rat offspring. Methods. Forty timed-pregnant Wistar rats were divided into four groups: A) Kind+/Seiz+, pregnant kindled (induced two weeks prior to pregnancy) rats that received repeated intraperitoneal (i.p.) pentylenetetrazol, PTZ injections on gestational days (GD) 14-19; B) Kind -/Seiz+, pregnant non-kindled rats that received PTZ injections on GD14-GD19; C) Kind+/Seiz-, pregnant kindled rats that did not receive any PTZ injections; and D) Kind-/Seiz -, the sham controls. Following birth, the pups were sacrificed on PD1 and PD14, and PSA-NCAM expression and localization in neonates hippocampi were analyzed by Western blots and immunohistochemistry. Results: Our data show a significant down regulation of hippocampal PSA-NCAM expression in the offspring of Kind+/Seiz+ (p=0.001) and Kind -/Seiz+ (p=0.001) groups compared to the sham control group. The PSA-NCAM immunoreactivity was markedly decreased in all parts of the hippocampus, especially in the CA3 region, in Kind+/Seiz+ (p=0.007) and Kind-/Seiz+ (p=0.007) groups newborns on both PD1 and 14. Conclusion: Our findings demonstrate that maternal seizures but not kindling influence the expression of PSA-NCAM in the offsprings hippocampi, which may be considered as a factor for learning/memory and cognitive impairments reported in children born to epileptic mothers. © 2012 Rajabzadeh et al.; licensee BioMed Central Ltd.


Ajami B.,Harvard University | Ghazvini K.,Ghaem Hospital | Movahhed T.,Harvard University | Ariaee N.,Bu Ali Research Institute | Makarem S.,Mashhad University of Medical Sciences
Iranian Red Crescent Medical Journal | Year: 2012

Background: Dental unit waterline system is considered potential source for contamination with Legionella species. The aim of this study was to determine if contamination of a dental unit water line system by Legionella pneumophila serogroup1 in the Mashhad School of Dentistry occurred in 2009. Methods: A total of 52 dental units were selected from all clinical departments of the Mashhad School of Dentistry. Samples of water were collected from outlets of water/air spray, high-speed dental hand pieces and water cup fillers. Samples were tested via the ELISA method. Results: At the beginning of the work day, a total of 36.1 percent of dental units were contaminated by Le-gionella pneumophila serogroup 1. Conclusion: Infection control of the dental unit water line system regarding legionella in the Mashhad School of Dentistry is a challenge and engineering controls should be used in contaminated clinics. © Iranian Red Crescent Medical Journal.


Spotin A.,Shahid Beheshti University of Medical Sciences | Majdi M.M.A.,Mashhad University of Medical Sciences | Sankian M.,Bu Ali Research Institute | Varasteh A.,Bu Ali Research Institute
Parasitology Research | Year: 2012

Cystic echinococcosis (hydatidosis) is a zoonotic helminthic disease of human and other intermediated hosts wherein infection is caused by the larval stages of tapeworm Echinococcus granulosus. Growth of the larval stage is formed throughout the internal organs, the liver and lung, causing their destruction. Important pathways are unknown about suppression and survival of cysts in human body. In this study, apoptotic bifunctional effects are evaluated in relationship between host and parasite in cystic echinococcosis. Human lymphocytes were treated with hydatid fluid (HF). After 6 h of exposure, caspase-3 activity was measured by fluorometric assay in the HF-treated lymphocytes and control cells. Also, the expression of Bax (as pro- apoptotic protein) and Bcl-2 (an anti-apoptotic protein) mRNA was assessed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after 12 h of exposure. For surveying of apoptosis-inducing ligands TNF-related apoptosis-inducing ligand and Fas-L, germinal layer and accompaniment peripheral tissues as healthy control were separated by scalpel from each cyst in sterile condition, then were assess by semiquantitative RT-PCR method in mRNA expression. Both the ratio of Bax/Bcl-2 mRNA expression and caspase-3 activity were higher in the fertile fluid-treated lymphocytes relative to infertile fluidtreated lymphocytes and control group versus the expression level of apoptosis-inducing ligands having a relatively high level in germinal layer of infertile cyst in comparison to fertile cyst and healthy tissue. Apoptosis of germinal layer of fertile cysts is possibly one of the suppression mechanisms in hydatidosis patients, in contrast to lymphocytes apoptosis by modulator of hydatid fluid, one of the hydatid cyst survival mechanisms. © Springer-Verlag 2011.


PubMed | Mashhad University of Medical Sciences, Bu Ali Research Institute, a Immunology Research Center, d Medical Toxicology Research Center and Pharmacy School and and c Medical Toxicology Research Center
Type: Journal Article | Journal: Journal of immunotoxicology | Year: 2016

The immunotoxic effects of the isoquinoline alkaloid berberine (BBR) were investigated in Balb/c mice. Here, BBR was administered daily by intraperitoneal injection at doses of 5 and 10mg/kg for 14 days. Following the exposure, host spleen weight, cellularity and histopathology, as well as delayed-type hypersensitivity (DTH) responses, hemagglutination titers (HA), spleen cell subtype profiles, splenocyte cytokine production and lymphocyte proliferation were studied in all of the test groups of animals. The results showed that the high dose of BBR (10mg/kg) could suppress both cellular and humoral immune functions in the treated hosts. BBR at 5mg/kg only appeared to impact on DTH responses and lymphoproliferation. Based on the finding here, it would seem that BBR has effective immunosuppressive properties. Mechanistic studies are required to determine exactly how this material is acting to impart many of the immunotoxic effects demonstrated here. At the same time, further research should also be performed on BBR to further develop its potential use as an effective immunosuppressant or co-adjuvant for the treatment of diseases caused by an exaggerated or unwanted immune response.


Heravi R.M.,Ferdowsi University of Mashhad | Nasiraii L.R.,Islamic Azad University | Sankian M.,Bu Ali Research Institute | Kermanshahi H.,Ferdowsi University of Mashhad | Varasteh A.R.,Mashhad University of Medical Sciences
Biotechnology | Year: 2012

To date, lactobacilli are widely used in food industries and new probiotic products; hence these are considered as an attractive target for genetic modifications. This study was conducted to improve electroporation of probiotic lactobacilli which is a necessary prerequisite for genetic manipulations. Four strains of probiotic lactobacilli from different sources were grown in Man Rogosa and Sharp (MRS) broth medium containing glycine, as a cell wall weakening agent or a pulse of glycine for evaluation of glycine effect on electroporation efficiency. After evaluation of various parameters such as washing buffers, washing times, electric field strength, pulse duration and plasmid concentration, a practical electroporation protocol was presented to improve electrotransformation of lactobacilli in comparison with two standard protocols. This new protocol exhibited higher transformation efficiency (transformants/μg plasmids) than standard methods (p<0.05) with no differences between species (Lb. casei, Lb. crispatus, Lb. salivarus and L. rhamnosus). The pulse of glycine had no effect on the number of the transformants in three methods. In contrast to plasmid concentration, plasmid size had no influence on the transformation efficiency. The modified method enabled to transform plasmid into the resistant lactobacilli against transformation. These bacteria have potential for bioengineering research to improve special performance. © 2012 Asian Network for Scientific Information.


Assarehzadegan M.A.,Ahvaz Jundis hapur University of Medical science | Assarehzadegan M.A.,Bu Ali Research Institute | Sankian M.,Ahvaz Jundis hapur University of Medical science | Sankian M.,Bu Ali Research Institute | And 6 more authors.
Allergology International | Year: 2010

Background: The inhalation of Salsola kali pollen is an important cause of pollinosis during summer and early fall throughout desert and semi-desert areas. Sal k 1 has been previously reported as a major allergen of S. kali pollen. In this study, we produced the recombinant Sal k 1 and also its low IgE-binding mutant form. We further compared the IgE binding ability of these two recombinant molecules. Methods: The recombinant Sal k 1 and its low IgE-binding variant, obtained by three amino acid exchanges (R142→S, P143→A, D144→V), were cloned and expressed in E. coli, as proteins fused with thioredoxin and His-tags, and then purified by Ni2+ affinity chromatography. The IgE-binding capacity of the wild-type and mutated rSal k 1 was compared using immunoblotting, ELISA and inhibition assays by ten sera from S. kali allergic patients. Moreover, in vivo IgE-reactivity was investigated by the skin prick test. Results: Both the recombinant and the mutated form of Sal k 1 were expressed in E. coli at a relatively high amount and soluble form. All sera recognized rSal k 1 via immunoassay analysis. In addition, inhibition assays demonstrated that the purified rSal k 1 was similar to its counterpart in the crude extract. The mutated rSal k 1 exhibited a reduced IgE-binding capacity against wild-type rSal k 1. Conclusions: This study demonstrates that purified rSal k 1 is comprised of IgE-epitopes similar to that of its natural counterpart and that the mutated variant showed a reduced IgE-binding capacity based on in vitro assays and in vivo provocation testing. © 2010 Japanese Society of Allergology.


PubMed | Bu Ali Research Institute and Azad University of Mashhad
Type: Journal Article | Journal: Reports of biochemistry & molecular biology | Year: 2016

Recently, reports have indicated a role for the membrane form of Toll-like Receptor 2 (TLR2) in asthma pathogenesis. In this study we examined soluble TLR2 levels in serum and sputum of asthmatic and healthy subjects.Serum and sputum samples were obtained from 33 asthmatic and 19 healthy subjects. The asthmatics were classified into four groups according to the Global Initiative for Asthma. A sandwich ELISA was developed to measure soluble TLR2 (sTLR2) in serum and sputum. TLR2 mRNA expression was determined by semi-quantitative RT-PCR of all sputum samples.The mean sTLR2 levels from serum and sputum of asthmatics were significantly lower than those from healthy subjects. Moreover, sTLR2 concentration decreased concomitantly with asthma severity. The differences observed, however, were not statistically significant. TLR2/GAPDH mRNA of sputum leukocytes was also significantly lower in asthmatics than in healthy subjects.This study demonstrated for the first time thatsTLR2 levels are lower in serum and sputum samples from asthmatic than from healthy subjects, and this could be an indicator of TLR2 expression. We also found that sTLR2 concentration in serum decreased concomitantly with an increase of asthma severity clinical score .


PubMed | Bu Ali Research Institute
Type: Journal Article | Journal: Journal of immunotoxicology | Year: 2014

Rose Bengal (RB) has been used as a safe agent in clinical diagnosis. In addition, it is used as a photodynamic sensitizer for removing microorganisms and cancer cells. Recently, its preferential toxicity after direct exposure to cancer cells was proven. The present study focuses on anti-cancer and anti-inflammatory activities of RB. The toxicity of RB against AGS gastric cancer and NIH 3T3 fibroblast cell lines was studied using an MTT assay. Patterns of any cell death among the AGS cells were defined using Annexin-V and PI staining. In addition, the effect of RB on nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production induced by lipopolysaccha-ride in J774A.1 macrophages was determined. Modulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions in the macrophages was also evaluated by Western blots. The results showed that AGS cells exhibited significant concentration-dependent decreases in growth in response to RB; these cells showed a greater growth inhibition than did non-malignant 3T3 cells, suggesting that anti-growth activity of RB could be cell-specific. Moreover, AGS cells exposed to RB exhibited a significant increase in apoptosis; only at high RB doses did the cells display significant levels of necrosis. While RB also caused a modest decrease in the growth of J774A.1 macrophages, the cells displayed remarkable decreases in NO production and iNOS expression without significant concurrent modulation in PGE(2) production or COX-2 expression. The data from this study appears to suggest that RB differentially impacts on transformed cell lines, preferentially suppresses growth of a gastric cancer cell line through induction of apoptosis, and induces changes in cells that could reflect potential anti-inflammatory effects that might be induced in situ.


PubMed | Bu Ali Research Institute
Type: Journal Article | Journal: Journal of immunotoxicology | Year: 2015

Artemisia species are important medicinal plants throughout the world. The present in vitro study, using a sesquiterpene lactone-bearing fraction prepared from Artemisia khorassanica (SLAK), sought to investigate immunomodulatory/anti-inflammatory properties of this plant and elucidate potential underlying mechanisms for the actions. Effects of the SLAK on mitogen-induced murine splenocyte proliferation and interleukin (IL)-4 and interferon (IFN)- secretion were evaluated. To assess anti-inflammatory activities, levels of inducible of nitric oxide (NO) and prostaglandin E2 (PGE2), as well as expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in peritoneal macrophages was examined. The results showed that SLAK noticeably was capable of suppressing PHA/LPS-stimulated splenocyte proliferation and of up-regulating production of the T-helper (TH)-2 cell cytokine IL-4 while down-regulating formation of TH1 IFN. In addition, while SLAK caused negligible proliferation inhibition, peritoneal macrophages displayed considerable decrease in NO and PGE2 production along with iNOS and COX-2 expression. The current experiment shows Artemisia khorasanica - a traditionally used herb - may have immunomodulatory and anti-inflammatory effects. It is anticipated that the ingredients may be employed as therapeutic candidates in the regulation of some immune responses implicated in various conditions and ailments.


Amini A.,Bu Ali Research Institute | Sankian M.,Mashhad University of Medical Sciences | Assarehzadegan M.A.,Ahvaz Jundishapur University of Medical Sciences | Vahedi F.,Razi Vaccine and Serum Research Institute | Varasteh A.,Mashhad University of Medical Sciences
Molecular Biology Reports | Year: 2011

The inhalation of Chenopodium album (C. album) pollen has been reported as an important cause of allergic respiratory symptoms. The aim of this study was to produce the recombinant profilin of C. album (rChe a 2) pollen and to investigate its cross-reactivity with other plant-derived profilins based on potential conformational epitopes and IgE reactivity analysis. Che a 2-coding sequence was cloned, expressed, and purified using one step metal affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted IgE potential epitopes among rChe a 2 and other plantderived profilins. Immunodetection and inhibition assays using sixteen individual sera from C. album allergic patients demonstrated that purified rChe a 2 could be the same as that in the crude extract. The results of inhibition assays among rChe a 2 and other plant-derived profilins were in accordance with those of the homology of predicted conserved conformational regions. In this study, amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins may depend on predicted potential IgE epitopes. © Springer Science+Business Media B.V. 2010.

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