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Kholiya K.,BT Kumaon Institute of Technology | Chandra J.,N.I.T.
Applied Physics A: Materials Science and Processing | Year: 2013

A simple theoretical model is developed to study the pressure-volume- temperature relationship and applied for nanocrystalline forsterite in the temperature range 300-1573 K and pressure range 0-9.6 GPa. The results obtained with the present model are in quite close agreement to the experimental values. The model is therefore extended to study the variation of bulk modulus and the coefficient of volume thermal expansion under high pressure and high temperature. The present study also reveals that the quasi-harmonic approximation, i.e., the product of bulk modulus and the coefficient of volume thermal expansion as constant, is valid at least up to the temperature 1573 K and pressure 9.6 GPa in case of nanocrystalline forsterite. © 2013 Springer-Verlag Berlin Heidelberg.


Kumar V.,Maharshi Dayanand University | Saxena J.,BT Kumaon Institute of Technology | Tiwari S.K.,Maharshi Dayanand University
Archives of Microbiology | Year: 2016

Haloarchaea grow in the extreme environment, such as high salt concentration, and secrete antimicrobial peptides known as halocins. Identification of Haloferax larsenii strain HA1 was carried out using biochemical and molecular methods. Strain HA1 was found as a strict aerobe, catalase positive and Gram negative. It was able to grow optimally at 15 % NaCl (w/v), 42 °C and pH 7.2. Strain HA1 was sensitive to bile acid, was resistant to chloramphenicol and could not utilize arginine. Halocin, produced by strain HA1, was stable up to 100 °C and in a pH range of 5.0–9.0. Antimicrobial activity was not affected by organic solvents, surfactants and detergents, but it was completely lost in the presence of proteinase K, suggesting proteinaceous nature of the compound. It was halocidal against indicator strain Hfx. larsenii HA10. The molecular weight of halocin HA1 was found to be ~14 kDa. These properties of halocin HA1 may be applicable to the preservation of salted foods. © 2015, Springer-Verlag Berlin Heidelberg.


Kholiya K.,BT Kumaon Institute of Technology | Chandra J.,P.A. College
Modern Physics Letters B | Year: 2015

On the basis of the thermal equation-of-state a simple theoretical model is developed to study the pressure dependence of melting temperature. The model is then applied to compute the high pressure melting curve of 10 metals (Cu, Mg, Pb, Al, In, Cd, Zn, Au, Ag and Mn). It is found that the melting temperature is not linear with pressure and the slope dTm/dP of the melting curve decreases continuously with the increase in pressure. The results obtained with the present model are also compared with the previous theoretical and experimental data. A good agreement between theoretical and experimental result supports the validity of the present model. © 2015 World Scientific Publishing Company.


Chaudhary P.P.,National Dairy Research Institute | Chaudhary P.P.,Banasthali University | Sirohi S.K.,National Dairy Research Institute | Saxena J.,BT Kumaon Institute of Technology
Gene | Year: 2012

The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further subjected to Amplified Ribosomal DNA Restriction Analysis (ARDRA) by using HaeIII enzyme. A total of 108 clones were examined, and the analysis revealed 16 phylotypes. Out of sixteen phylotypes, nine phylotypes belong to the uncultured group of methanogens, and the rest of seven phylotypes belong to the order Methanomicrobiales, Methanococcales and Methanobacteriales. Out of the 108 rDNA clones, 66 clones which constitute 61.1% of the total clone representing 9 phylotypes, show less than 97% sequence similarity with any of the cultured strain of methanogens. The second largest group of clones (24 clones) represented by four phylotypes show a sequence similarity ranging from 91% to 99% with Methanomicrobium mobile strain of methanogens. The third group of 16S rDNA clones clustered along with M. burtonii strain of methanogens. This group consists of 6 clones and constitutes about 5.5% of the total clones and represented by only single phylotype. Fourth and fifth clusters of 16S rDNA clones consist of 5 and 7 clones respectively, and these were matched with Methanobrevibacter gottschalkii and Methanobrevibacter rumanatium strain of methanogens and constitute about 4.6% and 6.4% of the total clones. © 2011 Elsevier B.V.


Saxena J.,BT Kumaon Institute of Technology | Minaxi,Banasthali University | Jha A.,Ashok and Rita Patel Institute of Integrated study
Clean - Soil, Air, Water | Year: 2014

Available phosphorus is limiting in most of cultivable soils in several parts of India, including Rajasthan. The beneficial effects of rhizosphere microorganisms such as phosphate solubilizing bacteria (PSB) have been found to increase in the presence of mycorrhizal symbiosis. In view of this, the synergistic effect of an arbuscular mycorrhizal fungus (AMF), Glomus etunicatum and an indigenous PSB strain, Burkholderia cepacia BAM-6 was studied on wheat plants grown in pots containing soil with low available P to see their potential to be used as bio-inoculants in semi-arid regions. The dual inoculation (AMF+PSB) augmented all growth and yield parameters studied in comparison to the individual inoculations with AMF and PSB. Crop yield and N concentration were enhanced >50 and 90%, respectively, following the dual inoculation, and percent root colonization by AMF and rhizosphere population of PSB were also found to increase with time in soil. This investigation reveals that the PSB strain B. cepacia BAM-6 interacted synergistically with AMF in promoting plant growth and nutrient uptake of wheat plants, therefore show great promise to be used as biofertilizer for wheat crop grown in arid to semi arid regions. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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