BT Kumaon Institute of Technology

Dwārāhāt, India

BT Kumaon Institute of Technology

Dwārāhāt, India
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Jain R.,Indian Institute of Technology Delhi | Saxena J.,BT Kumaon Institute of Technology | Sharma V.,Banasthali University
Mycology | Year: 2017

Low phosphorus availability in soil is one of the major causes that hamper crop yield. The main objectives of this work were to isolate efficient phosphate-solubilising strains from semi-arid soil and to check their efficiency to solubilise different inorganic phosphorus forms (viz., dicalcium phosphate (DCP), tricalcium phosphate and Udaipur rock phosphate) at different temperatures. Initially, 40 strains were isolated from rhizosphere soil of Sesamum indicum grown in semi-arid region of Rajasthan, India, of which 24 isolates showed phosphate-solubilising ability. Further, screening of these isolates for their phosphate solubilisation efficiency in solid media and broth led to the selection of two most competent isolates viz. SI32 and SI39. Both the isolates were effective in solubilising phosphorus at wide range of temperatures with different inorganic insoluble phosphorus sources. Amongst various insoluble phosphate sources tested, DCP was solubilised the most at all the temperatures but the performance was especially good in the range of 25–35°C. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Chaudhary P.P.,National Dairy Research Institute | Chaudhary P.P.,Banasthali University | Sirohi S.K.,National Dairy Research Institute | Saxena J.,BT Kumaon Institute of Technology
Gene | Year: 2012

The molecular diversity of rumen methanogens was investigated by 16S rDNA gene library prepared from the rumen contents obtained from Murrah buffaloes in India. Genomic DNA was isolated from adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the positive clones were selected assuming based on blue-white screening and sequenced. Positive clones were reamplified and the resulting PCR products were further subjected to Amplified Ribosomal DNA Restriction Analysis (ARDRA) by using HaeIII enzyme. A total of 108 clones were examined, and the analysis revealed 16 phylotypes. Out of sixteen phylotypes, nine phylotypes belong to the uncultured group of methanogens, and the rest of seven phylotypes belong to the order Methanomicrobiales, Methanococcales and Methanobacteriales. Out of the 108 rDNA clones, 66 clones which constitute 61.1% of the total clone representing 9 phylotypes, show less than 97% sequence similarity with any of the cultured strain of methanogens. The second largest group of clones (24 clones) represented by four phylotypes show a sequence similarity ranging from 91% to 99% with Methanomicrobium mobile strain of methanogens. The third group of 16S rDNA clones clustered along with M. burtonii strain of methanogens. This group consists of 6 clones and constitutes about 5.5% of the total clones and represented by only single phylotype. Fourth and fifth clusters of 16S rDNA clones consist of 5 and 7 clones respectively, and these were matched with Methanobrevibacter gottschalkii and Methanobrevibacter rumanatium strain of methanogens and constitute about 4.6% and 6.4% of the total clones. © 2011 Elsevier B.V.

Minaxi,Banasthali University | Saxena J.,Bt Kumaon Institute Of Technology | Chandra S.,Banasthali University | Nain L.,Indian Agricultural Research Institute
Journal of Soil Science and Plant Nutrition | Year: 2013

The present communication is an attempt to explore the synergistic effect of two indigenous phosphate solubilizing bacteria (PSB) (Pseudomonas fluorescens BAM-4 and Burkholderia cepacia BAM-12) and arbuscular mycorrhizal (AM) fungus, Glomus etunicatum on growth, yield and nutrient uptake of wheat plants grown in nutrient deficient soils in pots amended with tricalcium phosphate (TCP). A significant increase in growth, yield and nutrient uptake of wheat plants was noticed and both strains of PSB interacted positively with AM fungus towards all growth parameters studied. A remarkable enhancement of seed yield was recorded notably by 92.8% and an increase in percent root colonization of AM and PSB population in soil was also recorded with time.

Jha A.,Ashok and Rita Patel Institute of Integrated Study | Saxena J.,BT Kumaon Institute of Technology | Sharma V.,Banasthali University
Communications in Soil Science and Plant Analysis | Year: 2013

Ten phosphate-solubilizing bacterial strains belonging to genera Pseudomonas, Burkholderia, Enterobacter, Serratia, Klebsiella, and Aeromonas were tested for mineral phosphate solubilization activity in Pikovskaya's broth using different phosphate sources at four temperatures (15, 25, 35, and 45 οC). Dicalcium and tricalcium phosphate were solubilized more effectively (≥1000 mg L-1) than ferric and rock phosphate (≥100 mg L-1) and 35 °C was found to be the optimum temperature. Although Klebsiella and Aeromonas spp. are well known for their dinitrogen (N2)-fixing ability, to the best of our knowledge, this is the first report of inorganic phosphate solubilization by Klebsiella terrigena and Aeromonas vaga. Interestingly, A. vaga BAM-77 is the most efficient strain at solubilizing inorganic phosphorus (P) even in the presence of 8% sodium chloride (NaCl) at pH 10. These findings indicate that all four strains are efficient P solubilizers under variable conditions of temperature, pH, and P source and thus can be recommended for P fertilization in different soils. © 2013 Copyright Taylor & Francis Group, LLC.

Saxena J.,BT Kumaon Institute of Technology | Minaxi,Banasthali University | Jha A.,Ashok and Rita Patel Institute of Integrated Study
Clean - Soil, Air, Water | Year: 2014

Available phosphorus is limiting in most of cultivable soils in several parts of India, including Rajasthan. The beneficial effects of rhizosphere microorganisms such as phosphate solubilizing bacteria (PSB) have been found to increase in the presence of mycorrhizal symbiosis. In view of this, the synergistic effect of an arbuscular mycorrhizal fungus (AMF), Glomus etunicatum and an indigenous PSB strain, Burkholderia cepacia BAM-6 was studied on wheat plants grown in pots containing soil with low available P to see their potential to be used as bio-inoculants in semi-arid regions. The dual inoculation (AMF+PSB) augmented all growth and yield parameters studied in comparison to the individual inoculations with AMF and PSB. Crop yield and N concentration were enhanced >50 and 90%, respectively, following the dual inoculation, and percent root colonization by AMF and rhizosphere population of PSB were also found to increase with time in soil. This investigation reveals that the PSB strain B. cepacia BAM-6 interacted synergistically with AMF in promoting plant growth and nutrient uptake of wheat plants, therefore show great promise to be used as biofertilizer for wheat crop grown in arid to semi arid regions. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Kumar V.,Maharshi Dayanand University | Saxena J.,BT Kumaon Institute of Technology | Tiwari S.K.,Maharshi Dayanand University
Archives of Microbiology | Year: 2016

Haloarchaea grow in the extreme environment, such as high salt concentration, and secrete antimicrobial peptides known as halocins. Identification of Haloferax larsenii strain HA1 was carried out using biochemical and molecular methods. Strain HA1 was found as a strict aerobe, catalase positive and Gram negative. It was able to grow optimally at 15 % NaCl (w/v), 42 °C and pH 7.2. Strain HA1 was sensitive to bile acid, was resistant to chloramphenicol and could not utilize arginine. Halocin, produced by strain HA1, was stable up to 100 °C and in a pH range of 5.0–9.0. Antimicrobial activity was not affected by organic solvents, surfactants and detergents, but it was completely lost in the presence of proteinase K, suggesting proteinaceous nature of the compound. It was halocidal against indicator strain Hfx. larsenii HA10. The molecular weight of halocin HA1 was found to be ~14 kDa. These properties of halocin HA1 may be applicable to the preservation of salted foods. © 2015, Springer-Verlag Berlin Heidelberg.

Sharma M.K.,Amrapali Institute | Vaisla K.S.,BT Kumaon Institute of Technology
Proceedings - 2014 4th International Conference on Communication Systems and Network Technologies, CSNT 2014 | Year: 2014

Ever since the term e-Governance became popular a few years back, governments around the world are trying to introduce technology into how they function and deliver services to citizens. While the Internet really accelerated the use of e-Governance services like on line banking in India, the ultimate vision of e-Governance is to offer government services online to a common man in a quick and cost effective manner, here this paper advocate how we can use power of 'cloud computing' to offer many government services without having costly infrastructure on user end, By its compelling cost-benefits and quick-and-easy adoption. G-Cloud (Governance using cloud) concept of this paper will help to make a huge difference, how governments may deliver citizen services in towns and villages of India. © 2014 IEEE.

Kholiya K.,Bt Kumaon Institute Of Technology | Chandra J.,N.I.T.
Applied Physics A: Materials Science and Processing | Year: 2013

A simple theoretical model is developed to study the pressure-volume- temperature relationship and applied for nanocrystalline forsterite in the temperature range 300-1573 K and pressure range 0-9.6 GPa. The results obtained with the present model are in quite close agreement to the experimental values. The model is therefore extended to study the variation of bulk modulus and the coefficient of volume thermal expansion under high pressure and high temperature. The present study also reveals that the quasi-harmonic approximation, i.e., the product of bulk modulus and the coefficient of volume thermal expansion as constant, is valid at least up to the temperature 1573 K and pressure 9.6 GPa in case of nanocrystalline forsterite. © 2013 Springer-Verlag Berlin Heidelberg.

Kholiya K.,Bt Kumaon Institute Of Technology | Chandra J.,P.A. College
Modern Physics Letters B | Year: 2015

On the basis of the thermal equation-of-state a simple theoretical model is developed to study the pressure dependence of melting temperature. The model is then applied to compute the high pressure melting curve of 10 metals (Cu, Mg, Pb, Al, In, Cd, Zn, Au, Ag and Mn). It is found that the melting temperature is not linear with pressure and the slope dTm/dP of the melting curve decreases continuously with the increase in pressure. The results obtained with the present model are also compared with the previous theoretical and experimental data. A good agreement between theoretical and experimental result supports the validity of the present model. © 2015 World Scientific Publishing Company.

Kholiya K.,Bt Kumaon Institute Of Technology | Chandra J.,N.I.T.
AIP Conference Proceedings | Year: 2013

In the present investigation the B1-B2 structural phase transition and elastic properties of BaSe has been predicted using the simple potential model considered up to third nearest neighbor interaction. The calculated values of B1-B2 phase transition pressure, equation of state (Compression curve), bulk modulus, its first order pressure derivative and second order elastic constants are given along with the available experimental and other theoretical values. The results achieved in the present study are found in good agreement with the available experimental data. © 2013 AIP Publishing LLC.

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