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Chatzovoulos P.,National and Kapodistrian University of Athens | Tsoupras A.B.,National and Kapodistrian University of Athens | Samiotaki M.,Protein Chemistry Laboratory Bsrc Alexander Fleming | Panayotou G.,Protein Chemistry Laboratory Bsrc Alexander Fleming | And 2 more authors.
European Journal of Inflammation | Year: 2011

Leishmania spp are obligate intracellular protozoan parasites of the mononuclear phagocyte system that cause a spectrum of diseases known as leishmaniasis. Platelet Activating Factor (PAF) and some PAF-antagonists seem to play a key role in Leishmania infection. In this article we detected for the first time the specific activities of PAF basic metabolic enzymes, PAF cholinephosphotransferase (PAF-CPT) and Lyso-PAF-acetylotransferase (Lyso-PAF-AT), in two species of Leishmania, namely Leishmania major (L. major) and Leishmania infantum (L. infantum). Specific activity of PAF-CPT of L. major homogenates was substantially higher than that of L. infantum homogenates, while Lyso-PAF-AT specific activities of the homogenates of both species were detected in the same levels. In addition, PAF-like molecules were detected in cells and their culture medium of both species. These results show that PAF-biosynthesis exists and varies between Leishmania species, findings that are in correlation with the general idea that the presence of PAF in several Leishmania species is implicated in Leishmania infection. Copyright © by BIOLIFE, s.a.s. Source

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