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Guimarães, Portugal

Requicha J.F.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Requicha J.F.,University of Tras os Montes e Alto Douro | Requicha J.F.,Bs Pt Government Assoc Laboratory | Viegas C.A.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | And 10 more authors.
Tissue Engineering - Part A | Year: 2014

Human and canine periodontium are often affected by an inflammatory pathology called periodontitis, which is associated with severe damages across tissues, namely, in the periodontal ligament, cementum, and alveolar bone. However, the therapies used in the routine dental practice, often consisting in a combination of different techniques, do not allow to fully restore the functionality of the periodontium. Tissue Engineering (TE) appears as a valuable alternative approach to regenerate periodontal defects, but for this purpose, it is essential to develop supportive biomaterial and stem cell sourcing/culturing methodologies that address the complexity of the various tissues affected by this condition. The main aim of this work was to study the in vitro functionality of a newly developed double-layer scaffold for periodontal TE. The scaffold design was based on a combination of a three-dimensional (3D) fiber mesh functionalized with silanol groups and a membrane, both made of a blend of starch and poly-ε-(caprolactone). Adipose-derived stem cells (canine adipose stem cells [cASCs]) were seeded and cultured onto such scaffolds, and the obtained constructs were evaluated in terms of cellular morphology, metabolic activity, and proliferation. The osteogenic potential of the fiber mesh layer functionalized with silanol groups was further assessed concerning the osteogenic differentiation of the seeded and cultured ASCs. The obtained results showed that the proposed double-layer scaffold supports the proliferation and selectively promotes the osteogenic differentiation of cASCs seeded onto the functionalized mesh. These findings suggest that the 3D structure and asymmetric composition of the scaffold in combination with stem cells may provide the basis for developing alternative therapies to treat periodontal defects more efficiently. © Copyright 2014, Mary Ann Liebert, Inc. Source

Pereira D.R.,University of Minho | Pereira D.R.,Bs Pt Government Assoc Laboratory | Costa P.,University of Minho | Costa P.,Bs Pt Government Assoc Laboratory | And 2 more authors.
Archives of Clinical Neuropsychology | Year: 2015

The Symbol Digit Modalities Test (SDMT) isa widely used instrumentto assess information processing speed, attention, visual scanning, and tracking. Considering that repeated evaluations are a common need in neuropsychological assessment routines, we explored test-retest reliability and practice effects of two alternate SDMT forms with a short inter-assessment interval. A total of 123 university students completed the written SDMT version in two different time points separated by a 150-min interval. Half of the participants accomplished the same form in both occasions, while the other half filled different forms. Overall, reasonable test-retest reliabilities were found (r =.70), and the subjects that completed the same form revealed significant practice effects(p <.001, dz = 1.61), which were almost non-existentin thosefilling different forms. These forms were found to bemoderately reliable and toelicitasimilar performance across participants, suggesting their utilityinrepeated cognitive assessments when brief inter-assessment intervals are required. Source

Costa P.F.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Costa P.F.,Bs Pt Government Assoc Laboratory | Martins A.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Martins A.,Bs Pt Government Assoc Laboratory | And 6 more authors.
Tissue Engineering - Part B: Reviews | Year: 2014

Bone diseases and injuries are highly incapacitating and result in a high demand for tissue substitutes with specific biomechanical and structural features. Tissue engineering has already proven to be effective in regenerating bone tissue, but has not yet been able to become an economically viable solution due to the complexity of the tissue, which is very difficult to be replicated, eventually requiring the utilization of highly labor-intensive processes. Process automation is seen as the solution for mass production of cellularized bone tissue substitutes at an affordable cost by being able to reduce human intervention as well as reducing product variability. The combination of tools such as medical imaging, computer-Aided fabrication, and bioreactor technologies, which are currently used in tissue engineering, shows the potential to generate automated production ecosystems, which will, in turn, enable the generation of commercially available products with widespread clinical application. © 2014, Mary Ann Liebert, Inc. Source

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