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Fan L.,Jiangnan University | Li J.,Jiangnan University | Deng K.,Jiangnan University | Ai L.,Bright Dairy and Food Co.
Carbohydrate Polymers

Three polysaccharides (GLP-H, GLP-V and GLP-F) were obtained from Ganoderma lucidum by hot air drying, vacuum drying and vacuum freeze drying, respectively. Their chemical compositions were determined, and antioxidant activities were investigated on the basis of superoxide radical, hydroxyl radical, reducing power, DPPH free radical and Ferric-reducing antioxidant power (FRAP) assay. The results showed that three polysaccharides exhibited antioxidant activities in a concentration-dependent manner. Among three polysaccharides, GLP-F and GLP-V had the higher scavenging effects on hydroxyl radicals, superoxide radicals, DPPH free radical, and stronger reducing power than GLP-H. GLP-F showed the stronger antioxidant capacity than GLP-V and GLP-H in FRAP system. GLP-H and GLP-V showed an almost identical pattern in FRAP system (p > 0.05). However, GLP-V showed the stronger radical scavenging activities than GLP-H. Available data obtained in in vitro models suggested that vacuum freeze drying was an appropriate and effective treatment for obtaining the polysaccharide from G. lucidum. © 2011 Elsevier Ltd. All rights reserved. Source

Feng T.,Bright Dairy and Food Co. | Ye R.,University of Tennessee at Knoxville | Zhuang H.,Purdue University | Fang Z.,University of Queensland | Chen H.,Hefei University of Technology
Carbohydrate Polymers

In this research, thermal behavior and gelling interactions of Mesona Blumes gum (MBG)/rice starch mixture were extensively investigated. MBG/rice starch gel displayed significant endothermal and exothermal properties at different MBG concentrations, indicating essential interactions between MBG and rice starch. In addition, the gelling interaction between MBG and rice starch was studied by using hydrogen-bond forming agents (1,4-butanediol, ethane-1,2-diol, glycerol) and hydrogen-bond breaking agents (urea, tetramethyl urea, ethanol, methanol) on rheological spectra. The results indicated that the hydrogen bond between MBG, rice starch and water might be the major force of maintaining the complete structure of the mixed gel. Their hypothetic interactions have been schemed in computer using hyperchem 8.0. © 2012 Elsevier Ltd. Source

Li J.,Jiangnan University | Liu Y.,Jiangnan University | Fan L.,Jiangnan University | Ai L.,Bright Dairy and Food Co. | Shan L.,Jiangnan University
Carbohydrate Polymers

Four water-soluble polysaccharides fractions (ZSP1b, ZSP2, ZSP3c and ZSP4b) were isolated and purified from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao by DEAE-SepharoseCL-6B and SepharoseCL-6B column chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). Antioxidant activities of crude polysaccharide from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao (CZSP) and its purified fractions were investigated including superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging activity and reducing power. Antioxidant results showed that ZSP3c and ZSP4b containing more uronic acid had the stronger free radical scavenging activities than ZSP1b containing no uronic acid. Available data obtained with in vitro models suggested that uronic acid was an effective indicator of antioxidant activity of the samples. © 2010 Elsevier Ltd. All rights reserved. Source

Yu H.,Shanghai JiaoTong University | Zheng Y.,Bright Dairy and Food Co. | Li Y.,Shanghai JiaoTong University
International Journal of Food Properties

Moisture adsorption isotherms of freeze-dried bovine colostrum powder were obtained using the gravimetric method at three temperatures (15, 25, and 35°C) in the water activity range of 0.11-0.86. The moisture adsorption isotherms of freeze-dried bovine colostrum powder showed a sigmoid isotherm curve typical of the type II BET classification shape. The Guggenheim-Anderson- de Boer and Brunauer-Emmett-Teller models were recommended to fit experimental data well. The shelf life of freeze-dried bovine colostrum powder packaged in aluminium-laminated polyethylene pouches was evaluated for 243 days, and the storage stability was investigated when stored at 25°C and 50% relative humidity. A slight increase in moisture content, a linear increase in color difference, and a slight decrease in IgG concentration were found within 60 days storage under the above-mentioned conditions. © 2013 Copyright Taylor and Francis Group, LLC. Source

Li J.,Jiangnan University | Ai L.,Bright Dairy and Food Co. | Yang Q.,Jiangnan University | Liu Y.,Jiangnan University | Shan L.,Jiangnan University
International Journal of Biological Macromolecules

A water-soluble polysaccharide (ZP2a) was obtained by extraction with hot water followed by successive chromatographic purification using anion exchange and Sepharose CL-6B columns. A symmetrical narrow peak was obtained on high-performance gel permeation chromatography with an average molecular weight of 120,645. Da, which was named ZP2a. ZP2a is composed of rhamnose, arabinose, glucose and galactose in the molar ratio 1.3:1.7:0.3:1. Based on gas chromatographic, infrared spectral, methylation and NMR analyses, ZP2a was structurally characterized as 1,4-. d-Gal. pA residues interspersed with 1,2-. l-Rha. p and 1,2,4-. l-Rha. p residues. The branches were composed of 1,5-. l-Ara. f, 1,3,5-. l-Ara. f, 1,3-. l-Ara. f, 1,6-. d-Gal. p, 1,4,6-. d-Gal. p and 1,4-. d-Glc. p. The branches were attached to the backbone at the O-4 position of Rha. p residues. © 2012 Elsevier B.V. Source

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