Time filter

Source Type

Nottingham, United Kingdom

Lancashire L.J.,Paterson Institute for Cancer Research | Lancashire L.J.,Nottingham Trent University | Powe D.G.,University of Nottingham | Reis-Filho J.S.,Institute of Cancer Research | And 11 more authors.
Breast Cancer Research and Treatment

Gene expression microarrays allow for the high throughput analysis of huge numbers of gene transcripts and this technology has been widely applied to the molecular and biological classification of cancer patients and in predicting clinical outcome. A potential handicap of such data intensive molecular technologies is the translation to clinical application in routine practice. In using an artificial neural network bioinformatic approach, we have reduced a 70 gene signature to just 9 genes capable of accurately predicting distant metastases in the original dataset. Upon validation in a follow-up cohort, this signature was an independent predictor of metastases free and overall survival in the presence of the 70 gene signature and other factors. Interestingly, the ANN signature and CA9 expression also split the groups defined by the 70 gene signature into prognostically distinct groups. Subsequently, the presence of protein for the principal prognosticator gene was categorically assessed in breast cancer tissue of an experimental and independent validation patient cohort, using immunohistochemistry. Importantly our principal prognosticator, CA9, showed that it is capable of selecting an aggressive subgroup of patients who are known to have poor prognosis. © 2009 Springer Science+Business Media, LLC. Source

Cahan A.C.,Breast Institute | Palaia D.A.,Institute for Aesthetic Surgery and Medicine | Rosenberg M.,Institute for Aesthetic Surgery and Medicine | Bonanno P.C.,Institute for Aesthetic Surgery and Medicine
Annals of Plastic Surgery

The Box-To-X incision and closure is a novel technique for a skin-sparing mastectomy with immediate prosthetic reconstruction. The Box-To-X portal creates a 27% larger access for dissection as compared with a standard periareolar incision, while containing the entire scar within the limits of the original areola. Following nipple-areolar reconstruction and tattooing, a virtually scarless mastectomy results. Over a 54-month period, 159 skin-sparing mastectomies with immediate prosthetic reconstruction using the Box-To-X portal were performed on 98 patients. Allograft slings were used in all patients. Successful reconstruction was achieved in 93 of 98 patients (93%). Complications were observed in 23 (23%) patients. The most common complications were wound dehiscence, seroma, full-thickness necrosis along the edges of the "X," and wound infection. Removal of the implant was required in 5 patients as a result of persistent infection (5%). The low complication rate, adherence to accepted oncologic principles, and minimal scarring indicate that this technique is a sound addition to the armamentarium of both the breast oncologic surgeon and the reconstructive surgeon. Copyright © 2011 by Lippincott Williams & Wilkins. Source

Ayestaray B.,University of Nimes | Bekara F.,University of Nimes | Andreoletti J.-B.,Breast Institute
Aesthetic Plastic Surgery

The authors describe a vacuum-assisted skin grafting method to optimize graft take in the retroauricular region. This method is demonstrated after skin tumor resection of the retroauricular and mastoid areas. © 2012 Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery. Source

Jerjees D.A.,University of Nottingham | Green A.R.,University of Nottingham | Alshareeda A.,University of Nottingham | Macmillan R.D.,Breast Institute | And 2 more authors.
Breast Cancer Research and Treatment

The definition of Luminal-B subclass of breast cancer (BC) varies in literature. In this study, we have compared the proliferation status; assessed using KI67 labeling index (KI67-LI), and HER2-expression in estrogen receptor positive (ER+) BC to assess their impact on the biological and clinical characteristics of luminal-BC. 1547 (73.8 %) well-characterized clinically annotated stage I-III ER + BC were assessed for expression of KI67, HER2 (ASCO guidelines), and a large panel of relevant biomarkers (no = 37). 46.3 % of the cases show high KI67-LI (>13 %) and 8.4 % show HER2+ and both markers are positively associated with younger age, higher tumor grade and poorer outcome. High KI67-LI and HER2+ are associated with upregulation of ER-coactivators and proliferation-related markers and with downregulation of good prognostic markers. High KI67-LI is associated with larger size, advanced stage, and lymphovascular invasion (LVI) and with downregulation of luminal-enriched and DNA-damage repair markers. In contrast, HER2+ is associated with upregulation of ER-regulated proteins and E-cadherin. When analysis is restricted to high KI67-LI subgroup, HER2+ shows an association with upregulation of differentiation-associated proteins and E-cadherin. Conversely, within HER2+ class, high KI67-LI maintains its association with downregulation of differentiation-associated/luminal-enriched proteins. Outcome analyses indicate that both markers are independently associated with shorter survival but HER2+ is associated with a worse outcome. Although both are associated with high proliferation and poor prognosis within ER + BC, HER2+ is less frequent than high KI67-LI. Unlike KI67, HER2 seems to independently drive the aggressive behavior of ER+ tumors without downregulation of luminal proteins. © 2014 Springer Science+Business Media New York. Source

Ayestaray B.,University of Nimes | Ayestaray B.,Breast Institute | Bekara F.,University of Nimes | Andreoletti J.-B.,Breast Institute
Journal of Plastic, Reconstructive and Aesthetic Surgery

Background: Head and neck lymphoedema secondary to jugular lymphadenectomy is a severe issue, without efficient solution. Successful treatment of lymphoedema of the upper and lower limbs has become possible with supermicrosurgical lymphaticovenular anastomosis. The technique based on two end-to-side anastomosis is named π-shaped lymphaticovenular anastomosis. We have evaluated this method for chronic head and neck lymphoedema. Methods: From November 2010 to April 2011, four patients with a chronic head and neck lymphoedema were treated by π-shaped lymphaticovenular anastomosis. Three patients had a unilateral lymphoedema, and one patient had a bilateral lymphoedema. The mean age of the patients was 63.2 years (range, 46-77 years). The mean duration of the lymphoedema was 2.6 years (range, 1-5). Every patient was operated under local anaesthesia through a face-lift skin incision. One π-shaped lymphaticovenular anastomosis was performed at each operative site. Results: The average operative time to perform one π-shaped lymphaticovenular anastomosis was 1.9 h (range, 1.8-2.5). The calibre of lymphatic vessels used for lymphaticovenular anastomosis ranged from 0.3 to 0.7 mm (average, 0.5). A venous back-flow was found in seven lymphaticovenular anastomosis (70%). Three patients (75%) had a qualitative improvement of skin tissue and a significant circumferential reduction after surgery. The average circumferential differential reduction rate was 3.7% (range, 0.6-7.8) (p = 0.006). The average cross-sectional area differential reduction rate was 7.2% (range, 1.2-15.1) (p = 0.007). The average volume differential reduction rate was 6.9% (range, 2-14.8) (p = 0.05). Conclusions: The authors present a new option to treat head and neck lymphoedema. π-Shaped lymphaticovenular anastomosis is an effective method to reduce the severity of skin tissue fibrosis and lymphoedema volume. Further studies with larger groups of patients are required to confirm the outcome of this preliminary study. EBM Level = level 4. © 2012 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved. Source

Discover hidden collaborations