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Campioni F.,Brazilian Reference Center On Yersinia Spp Other Than stis | Falcao F.P.,Brazilian Reference Center On Yersinia Spp Other Than stis
Canadian Journal of Microbiology | Year: 2014

Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-field gel electrophoresis (PFGE) and none that have compared the capability of different enzymes on typing these strains. This study typed 51 Y. enterocolitica B1A strains isolated in Brazil and Chile by PFGE, testing the enzymes XbaI, NotI, and XhoI. The resulting dendrograms discriminated the strains in 47, 40, and 49 pulsotypes generated by the cleavage with the enzymes XbaI, NotI, and XhoI, respectively. The majority of the strains were grouped independently of their clinical or nonclinical origins. The high discriminatory power of PFGE confirmed the heterogeneity of B1A strains but could not divide the strains studied into clusters that differed in the frequency of some virulence genes as observed in studies using other methodologies. © National Research Council of Canada. All rights reserved.


Souza R.A.,Brazilian Reference Center On Yersinia Spp Other Than stis | Falcao J.P.,Brazilian Reference Center On Yersinia Spp Other Than stis
Advances in Experimental Medicine and Biology | Year: 2012

The genus Yersinia currently comprises 17 species. Yersinia pestis, Yersinia pseudotuberculosis and Yersinia enterocolitica are associated with human and animal diseases and, therefore, have been extensively studied. The remaining 14 Yersinia species have not been completely characterized and are generally considered non-pathogenic. However, published data have suggested that some of these so-called non-pathogenic Yersinia species may also be involved in human and animal diseases. One such species, named Y. massiliensis, was recently described and its pathogenic potential is unknown. Thus, it is important to define the clinical significance of this species. In this work, five Yersinia massiliensis strains were studied and compared to Y. enterocolitica 8081 pathogenic strain. The ability of Y. massiliensis strains to adhere and invade Caco-2 cells was studied. Furthermore, the presence of virulence genes inv, ail, ystA and virF was investigated by PCR. None of the Y. massiliensis strains harbored the virulence genes investigated and were barely able to invade Caco-2 cells. In addition, the adhesion percentages of the Y. massiliensis strains were 3.22 times lower than the values observed for Y. enterocolitica 8081. These results suggest that Y. massiliensis should be considered a non-pathogenic species. © 2012 Springer Science+Business Media New York.

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