Lima H.C.A.V.,Brazilian Field Epidemiology Training Program |
Porto E.A.S.,Brazilian Field Epidemiology Training Program |
Marins J.R.P.,Secretariat of Health Surveillance |
Alves R.M.,Foodborne and Waterborne Diseases Branch |
And 7 more authors.
Tropical Doctor | Year: 2010
Beriberi is caused by thiamine deficiency. Early 20th century epidemics in Japan were attributed to rice contaminated by citreoviridin mycotoxin. Our investigation of an outbreak of beriberi in Brazil showed an association of beriberi with the consumption of poor quality subsistence farming rice, although, unlike other investigators of this outbreak, we did not identify citreoviridin producing fungi in the implicated rice. Source
Tort L.F.L.,Oswaldo Cruz Institute FIOCRUZ |
Volotao E.d.M.,Oswaldo Cruz Institute FIOCRUZ |
de Mendonca M.C.L.,Oswaldo Cruz Institute FIOCRUZ |
da Silva M.F.M.,Oswaldo Cruz Institute FIOCRUZ |
And 6 more authors.
Journal of Clinical Virology | Year: 2010
Background: Group A rotavirus (RV-A) genotype PG9 has emerged as one of the leading causes of gastroenteritis in children worldwide and currently is recognized as one of the five most common genotypes detected in humans. High intragenotype diversity in G9 RV-A has been observed, and nowadays, based on the genetic variability of the VP7 gene, six different phylogenetic lineages and eleven sublineages were described. Objectives: To study the degree of genetic variation and evolution of Brazilian PG9 RV-A strains. Study design: Phylogenetic analysis of 19 PG9 RV-A strains isolated from 2004 to 2007 in five different Brazilian states was conducted using the NSP1, NSP3, NSP5, VP4 and VP7 genes. For the VP4 and VP7 genes, 3D protein structure predictions were generated to analyze the spatial distribution of amino acid substitutions observed in Brazilian strains. Results: Based on the phylogenetic analyses, all Brazilian strains clustered within lineage G9-III and P-3 for VP7 and VP4, respectively, and were classified as genotype A1, T1 and H1 for the NSP1, NSP3 and NSP5 genes, respectively. Interestingly, all the strains isolated in Acre State (Northern Brazil) formed a closely related cluster clearly separated from the other Brazilian and prototype strains with regard to the five genes studied. Unique amino acid substitutions were observed in Acre strains in comparison with the prototype and Brazilian strains. Conclusion: Inclusion of Acre strains in the phylogenetic analysis revealed the presence of a novel genetic variant and demonstrated a diversification of PG9 rotaviruses in Brazil. © 2010 Elsevier B.V. All rights reserved. Source