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Martinelli S.S.,Federal University of Santa Catarina | Cavalli S.B.,Federal University of Santa Catarina | Pires P.P.,Brazilian Enterprise for Agricultural Research | Proenca L.C.,Secretariat of Water Resources and Urban Environment | Proenca R.P.C.,Federal University of Santa Catarina
Journal of Culinary Science and Technology | Year: 2012

This study aimed to verify the level of drinking water consumption for the thawing of beef under running water in a restaurant. The consumption estimates were determined by obtaining tap water flows during five different days. The results revealed that consumption reached approximately 8,340 L of water to thaw 109.5 kg of beef. The average daily consumption reached 1,668 L of water for 21.9 kg of beef, approximately 76.2 L of water/kg of meat. The meat temperature and time consumed in thawing were, for the most part, above the recommended level for the meat temperature and thawing time. The average water flow was of 5.2 L/min, reaching 14.8 L/min. It was observed that there is a possibility of decreasing water consumption, especially by scheduling specific times to carry out the procedure. The information in this study can serve as a reference for restaurants when it comes to the reduction of water consumption. © 2012 Copyright Taylor & Francis Group, LLC.

Braga A.,Grande Rio University | Suita de Castro L.A.,Brazilian Enterprise for Agricultural Research | Poersch L.H.,Grande Rio University | Wasielesky W.,Grande Rio University
Aquaculture | Year: 2014

The present study evaluated spermatozoal capacitation in Farfantepenaeus paulensis. This process has direct applications in aquaculture, and it consists of the ionic, biochemical and morphological changes during the period that the spermatophore is stored or adhered to the thelycum. These changes make the spermatozoa capable of fertilization. The morphological changes of spermatozoal capacitation have been previously investigated only in sicyoniids and open-thelycum penaeids. Thus, this study is the first morphological account of a closed-thelycum penaeid. F. paulensis broodstock were captured offshore in southern Brazil, and spermatophores were collected from the terminal ampoule and from the thelycum of sexually mature females that had naturally copulated. The ultrastructure of the spermatozoal capacitation was investigated via transmission electron microscopy. Spermatozoa of F. paulensis show the following changes related to capacitation: (1) the chromatin became less condensed; (2) the acrosomal region became more electron-dense (3) the acrosomal cap became less concave; and (4) the subacrosomal region became much more electron-dense. These results demonstrate that the morphological changes in capacitated spermatozoa of F. paulensis are similar to those previously reported for open-thelycum penaeids, i.e., Litopenaeus species. Further studies on the capacitation process are required, especially to evaluate the interaction between biochemical and morphological changes. Such research could be useful for developing biotechnologies that will allow spermatozoal induction without storing the spermatophore in the thelycum and therefore allow in vitro larval production. © 2014 Elsevier B.V.

Diniz J.R.,University of Brasilia | Correa J.R.,University of Brasilia | Moreira D.D.A.,University of Brasilia | Fontenele R.S.,University of Brasilia | And 6 more authors.
Inorganic Chemistry | Year: 2013

This article describes a straightforward and simple synthesis of ionically tagged water-soluble Eu3+ and Tb3+ complexes (with ionophilic ligands) applied for bioimaging of invasive mammal cancer cells (MDA-MB-231). Use of the task-specific ionic liquid 1-methyl-3-carboxymethyl- imidazolium chloride (MAI·Cl) as the ionophilic ligand (ionically tagged) proved to be a simple, elegant, and efficient strategy to obtain highly fluorescent water-soluble Eu3+ (EuMAI) and Tb3+ (TbMAI) complexes. TbMAI showed an intense bright green fluorescence emission selectively staining endoplasmic reticulum of MDA-MB-231 cells. © 2013 American Chemical Society.

DE MOURA ZANINE A.,Federal University of Maranhao | NASCIMENTO D.,Federal University of Vicosa | DA SILVA W.L.,Sao Paulo State University | DE LANA SOUSA B.M.,Federal University of Sergipe | And 4 more authors.
Experimental Agriculture | Year: 2016

The objective of this study was to evaluate morphogenetic and structural characteristics of Guinea grass pastures subjected to two grazing frequencies (90 and 95% light interception) and two post-grazing heights (30 and 50 cm), during the rainy season and dry year. The leaf appearance rate varied only in the summer, the grazing pastures managed at 90/50 (light interception/post-grazing height) presented higher values in the leaf appearance rate (0.440 leaf/tiller.day), compared with those managed at 90/30 (0.275 leaf/tiller.day) and 95/50 (0.228 leaf/tiller.day), respectively. In the winter/beginning of the spring, pastures managed at 90/30 (0.03 cm/tiller.day) presented lower stem elongation rate in comparison with those managed at 90/50 (0.19 cm/tiller.day) and 95/30 (0.16 cm/tiller.day). Management strategies do not compromise the tissue flow in tillers and, consequently, can be utilized for the management of Guinea grass. Copyright © Cambridge University Press 2016

Monteiro G.E.R.,Eduardo Mondlane University | Bechara G.H.,Sao Paulo State University | Franzin A.M.,University of Sao Paulo | de Miranda Santos I.K.F.,University of Sao Paulo | de Miranda Santos I.K.F.,Brazilian Enterprise for Agricultural Research
Experimental and Applied Acarology | Year: 2011

Resistance to tick feeding has been previously shown to be an acquired, immunologically mediated phenomenon in goats, associated with cutaneous basophilia to nymphs of Amblyomma cajennense, the Cayenne tick, after repeated infestations. On the other hand, it is well known that antigen-presenting cells (APCs) play an important role in the host immune reaction to tick infestations. The most able APCs for Th cells are the well defined dendritic cells, mononuclear phagocytes and B-lymphocytes. Immunohistochemical analysis of draining lymph nodes of goats repeatedly infested with nymphs of the ixodid tick A. cajennense to search for APCs was done. Pre-scapular lymph nodes draining the tick attachment sites were collected 15 days after both the first and third infestations. Tick infestations resulted in increased number of CD21+ B lymphocytes in lymph nodes after the tertiary infestation. However, the number of CD11b+ and CD11c+ cells were not altered after the successive infestations. Lower numbers of CD11c+ cells had infiltrated lymph nodes responsible for draining the tick infested skin. These findings suggest that acquired immunity of goats against nymphs of A. cajennense is possibly established by B lymphocytes during the first infestation and that APCs may play a key role in this mechanism. © 2010 Springer Science+Business Media B.V.

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