Luba C.D.N.,Sao Paulo State University |
Boakari Y.L.,Sao Paulo State University |
Costa Lopes A.M.,Brazilian Anteaters Research and Conservation Institute |
da Silva Gomes M.,Brazilian Anteaters Research and Conservation Institute |
And 3 more authors.
The giant anteater (Myrmecophaga tridactyla) is considered vulnerable to extinction. Scientific data on the reproductive parameters of this species are scarce. Semen from eight free-ranging giant anteaters was collected to establish its characteristics and the effects of cooling and storage at 5°C after dilution with the BotuCrio extender without cryoprotectant. The ejaculate presented two distinct sequential fractions, including a whitish fraction, which was milky and rich in sperm cells, and a gel fraction, which was colorless, viscous, and azoospermic. The mean±standard error of the mean values of the seminal characteristics were as follows: volume of the first fraction, 0.75±0.1mL; motility, 75±2.9%; vigor, 3.2±0.3; sperm motility index, 68.8±4.3; concentration, 108.5 ± 13.4×106/mL; plasma membrane integrity index, 71±4.0%; spermatic defects detected using modified Karras staining, 35.5±3.3%; and spermatic alterations identified by differential interference contrast microscopy, 48.3±6.8%. During refrigeration, the semen presented decreasing motility from 0 to 18hours, sperm motility index decreased from 0 to 24hours, and vigor did not change in the first 6hours and then decreased to 18hours. © 2015 Elsevier Inc. Source