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Gomes M.G.T.,University of Tocantins | Macedo-Junior G.L.,University of Veterinary and Animal Sciences | Ferreira M.I.C.,Brazilian Agricultural Research Corporation Embrapa Goats and Sheep | Borges I.,University of Minas Gerais | And 3 more authors.
Small Ruminant Research | Year: 2014

The present work aimed to characterize the uterine involution after singleton and twin parturitions in Santa Inês ewes submitted to two nutritional diets. Sixteen pregnant pluriparous Santa Inês ewes, with age ranging from 3 to 5 years, were used. The animals were randomized into four experimental groups of four animals each. The experiment was a 2. ×. 2 factorial with number of lambs (singleton versus twin pregnancy) and diet (maintenance diet versus maintenance diet with 15% energy deficit). To determinate the number of fetuses, all ewes were submitted to an ultrasonographic evaluation at days 30, and checked at 45 and 60 days of pregnancy. Genital tract involution was followed by vaginal smear cytology and transrectal evaluation of the uterus by ultrasonography. Changes in cells from the vaginal wall were not useful to characterize the involution process of the genital tract in ewes, however leukocyte cells were. Ultrasonographic observations pointed to a longer period for reduction and stabilization of uterine size after twin parturition, and there was no alteration of the uterine involution process due to the level of nutritional restriction. © 2014 Elsevier B.V. Source

Oliveira M.E.F.,Sao Paulo State University | Ayres H.,Sao Paulo State University | Oliveira L.G.,Sao Paulo State University | Barros F.F.P.C.,Sao Paulo State University | And 5 more authors.
Theriogenology | Year: 2016

This study set out to investigate the efficiency of long-term estrus synchronization protocols and ovulatory follicle dynamics in ultrasonographically monitored Santa Inês ewes during lengthening (LD; September-October) and shortening photoperiods (SD; April-May), and the transitional period (TP; January). In addition, the influence of ovarian status (e.g., size of antral follicles and/or presence of corpora lutea) at the outset of the estrus synchronization protocols on the ensuing development of ovulatory follicles was examined. Seventy sexually mature Santa Inês ewes were subjected to one of the two estrus synchronization regimens; on Day 0 (random day of the estrous cycle or anovulatory period), the ewes were fitted with an intravaginal progesterone (P4)-releasing (controlled intrauterine drug release [CIDR]) device, which was left in place for 14 days (G-1CIDR, n = 35) or replaced on Day 7 (G-2CIDR, n = 35), and received an intramuscular injection of 10 mg of PGF2α. The ewes allocated to the G-1CIDR group had mean serum P4 concentrations less than 2 ng/mL during the last 4 days of the synchronization protocol. There were no differences (P > 0.05) in mean ovulation rates between the two protocols tested nor among the ewes varying in ovarian status or studied at different times of the year, but ovulations occurred ~12 hours later in the TP compared with the SD period (P < 0.05). Ovulatory follicles emerged earlier (P < 0.05) in the G-1CIDR group than in the G-2CIDR group (Day 8.3 ± 0.5 vs. 9.2 ± 0.4) and during LD (Day 7.1 ± 0.6) compared with the TP (Day 9.1 ± 0.6) and SD (Day 9.9 ± 0.5 of the protocol). In conclusion, the replacement of CIDR devices prevented the occurrence of lower-than-normal luteal phase levels of P4 at the end of the 14-day estrus synchronization protocol. However, although this procedure and seasonal influences altered certain growth characteristics of ovulatory follicles, there were no effects of these factors on the mean ovulation rate. © 2016 Elsevier Inc. Source

Parente T.M.A.L.,Federal University of Ceara | Reboucas E.L.,Federal University of Ceara | dos Santos V.C.V.,Brazilian Agricultural Research Corporation Embrapa Goats and Sheep | Barbosa F.C.B.,Federal University of Ceara | Zanin I.C.J.,Federal University of Ceara
Photodiagnosis and Photodynamic Therapy | Year: 2016

Background: Some authors have reported the antimicrobial action of photodynamic antimicrobial chemotherapy (PACT) on bacteria related to nosocomial infections but there are few studies evaluating PACT on Serratia marcescens grown as planktonic cultures or as biofilms. The purpose of this study was to analyze the S. marcescens resistance profile and its susceptibility to PACT. Methods: Initially, 55 S. marcescens strains isolated from environmental, oral and extra-oral infections were tested by antimicrobial resistance to cefotaxime (CTX), imipenem (IPM), ciprofloxacin (CIP), tobramycin (TOB) and doxycycline (DOX) using E-test®. Following, isolates grown as planktonic cultures or biofilms were submitted to PACT using the association of a light-emitting diode and toluidine blue (TBO). The E-test® results demonstrated intermediated sensitive strains to CTX, IMP, TOB, and DOX; and resistant strains to CTX, TOB, DOX and CIP. Also, CTX and IMP demonstrated variation when CLSI 2007 and CLSI 2015 were compared. Results: Planktonic cultures and biofilms submitted to PACT demonstrated counts varying from 1011 to 107 for planktonic cultures and 1010 to 107 for biofilms. There were no statistical differences in the results when planktonic cultures and biofilms were compared. Conclusions: Increase in the profile of S. marcescens resistance was observed when CLSI 2007 and CLSI 2015 were compared. Also, IMP remains as the drug with lower rate of resistance. Additionally, both S. marcescens planktonic cultures and early biofilms are susceptible to PACT under tested conditions. © 2016 Elsevier B.V. Source

Oliveira M.E.F.,Sao Paulo State University | Feliciano M.A.R.,Sao Paulo State University | D'Amato C.C.,Sao Paulo State University | Oliveira L.G.,Sao Paulo State University | And 5 more authors.
Animal Reproduction Science | Year: 2014

The primary goal of this study was to employ ultrasonography to examine the ovaries of ewes undergoing superovulatory treatment for correlations between antral follicular blood flow and ovarian responses/embryo yields. Five Santa Inês ewes were subjected to a short- (Days 0-6, Group 1) and five to a long-term progesterone-based protocol (Days 0-12, Group 2) to synchronize estrus and ovulations after the superovulatory treatment. Porcine FSH (pFSH, 200. mg) was administered in 8 decreasing doses over 4 days, starting on Days 4 and 10 in Groups 1 and 2, respectively. After CIDR removal, all ewes were bred by a ram and embryos were recovered surgically 7 days later. Transrectal ovarian ultrasonography was performed the day before and on all 4 days of the superovulatory treatment. Both an arbitrary-scale [(0) non-detectable; (1) small; (2) moderate; (3) intense blood flow] and quantitative analysis of the blood flow area were used to assess the follicular blood flow in color Doppler images. There were no significant correlations between the arbitrary blood flow scores and superovulatory responses in the ewes of the present study. However, there was a positive correlation between the quantitative estimates of follicular blood flow on the final day of the superovulatory treatment, and the number (DA: r= 0.68, P<. 0.05; DA/TA. ×. 100%: r= 0.85, P<. 0.05) and percentage (DA: r= 0.65, P<. 0.05; DA/TA. ×. 100%: r= 0.91, P<. 0.001) of unfertilized eggs (DA: Doppler area, TA: total area of the largest ovarian cross section). This experiment presents a commercially practical tool for predicting superovulatory outcomes in ewes and evidence for the existence of follicular blood flow threshold that may impinge negatively on oocyte quality when surpassed during hormonal ovarian superstimulation. © 2013 Elsevier B.V. Source

Sousa F.M.L.,Federal University of Ceara | Lobo C.H.,Federal University of Ceara | Menezes E.S.B.,Federal University of Ceara | Rego J.P.A.,Federal University of Ceara | And 9 more authors.
Reproduction in Domestic Animals | Year: 2014

Contents: This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42 weeks of age, 15 rams were subjected to semen collection and, 1 week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR. At slaughter, scrotal circumference and testicular weight were 27.5 ± 0.5 cm and 109.5 ± 6.0 g, respectively. Seminiferous tubule (ST) diameter was 188.3 ± 4.0 μm and each testis contained 1.9 ± 0.1 Sertoli cells (×109). Each Sertoli cell supported 0.1 ± 0.01 A spermatogonia, 3.0 ± 0.2 pachytene spermatocytes and 7.7 ± 0.5 round spermatids/tubule cross section. Daily sperm production reached 5.6 × 106 cells/g of testis parenchyma. Testis size appeared as indicative of ST diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells/testis. We detected 90.9 ± 9.6 spots per 2-D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22 kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expression of both genes was greater in the vesicular glands as compared to testis and epididymis. Pixel intensity for those proteins in the 2-D gels was significantly correlated with seminal vesicle weight. This is the first description of the basic reproductive aspects of Morada Nova rams, including protein profiles of their seminal plasma. These findings will allow a better understanding of their reproductive physiology © 2014 Blackwell Verlag GmbH. Source

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