Bra Bihar University

Muzaffarpur, India

Bra Bihar University

Muzaffarpur, India
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Kumari M.,Bra Bihar University
International Journal of Green Pharmacy | Year: 2012

Background: Clitoria ternatea L. is an important rare medicinal plant species with memory-enhancing ability used as crude drugs in many ayurvedic medicines. Objective: The objectives were as follows: A. To develop a protocol for rapid clonal propagation of the important medicinal climber, C. ternatea L., through in vitro tissue culture of embryo explants through callogenesis and organogenesis, and B. Antibacterial study of ethanolic extract of in vitro raised plant and callus mass against Pseudomonas aeruginosa (MTCC189), Bacillus subtilis (MTCC8), Escherichia coli (MTCC1), and Klebsiella pneumonia (MTCC3883), respectively. Materials and Methods: Explants were cultured on Murashige and Skoog′s (MS) medium supplemented with different concentrations and combinations of 6-benzylamino purine (BAP), (2,4-dichlorophenoxy acetic acid (2,4-D), and -naphthalene acetic acid (NAA) for shoot and root induction. The disc diffusion method was adopted for antimicrobial study of the plant extract. Results: The sub-cultured of callus on MS basal medium supplemented with BAP (2.5 mg/l) and NAA (0.5 mg/l) showed highest rate of shoot multiplication. In vitro shoots were rooted on to the MS basal medium supplemented with NAA (0.5 mg/l). The sub-culture of callus on MS basal medium supplemented with BAP (1 mg/l) and NAA (0.5 mg/l) showed highest rate of root multiplication from callus. The antibacterial activity of the ethanolic extracts of in vitro grown products of C. ternatea L. was found to have antimicrobial activity against all tested microorganisms. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, carbohydrates, and steroids in the in vitro grown products of C. ternatea L. Conclusion: An efficient protocol was developed for successful micropropagation and multiple plant regeneration of an important medicinal plant C. ternatea L. It is a widely used in ayurvedic medicine because of its multi-potent bioactive molecules and significant antibacterial properties.

Rai B.K.,Bra Bihar University | Ranjana,har University | Prakash P.,A.C.C. Cement | Lata P.,Central Hindu School
Oriental Journal of Chemistry | Year: 2012

The present paper is a report on the synthesis of some Co(II), Ni(II) and Cu(II) complexes by the condensation of 2 ethyl indole 3-carboxaldehyde with thiosemicarbazide hydrochloride using ethanol as reaction medium. The bonding pattern and structural studies of ligand and complexes have been carried out on the basis of elemental analyses, molar mass, IR spectra, electronic spectra, molar conductance and magnetic susceptibility measurements. The nonelectrolytic nature of the complexes has been proposed on the basis of their molar mass determination and conductance measurements. The ligand and their complexes have been screened in vitro for their antimicrobial activity. Some of the reported complexes were found to be quite active.

Rai B.K.,Bra Bihar University | Sharma K.K.,Rss College
Oriental Journal of Chemistry | Year: 2011

Neutral bidentate Schiff base ligand 3 4-diethyl isoquinoline semicrabazone [DIES] and its complexes of Co(II), Ni(II) and Cu(II) complexes have been prepared. The ligand as well as metal complexes were characterized by analytical and physicochemical methods such as elemental analyses, molar mass, IR, U.V., magnetic susceptibility, molar conductance measurements. On the basis of these studies, it is proposed that DIES acts as neutral bidentate ligand and coordination proposed through azomethine nitrogen and carbonyl oxygen of semicarbazone moiety. On the basis of electronic spectra and magnetic susceptibility measurements, it is proposed that complexes have monomeric octahedral geometry. The ligand as well as metal complexes were also screened for their antimicrobial study.

Rai B.K.,Bra Bihar University | Bimal K.,Rss College
Oriental Journal of Chemistry | Year: 2010

Complexes of the type [M(DMPS)2×2]. Where M=cobalt(II) nickel(II) and copper(II) DMPS =26 Dimethyl 4 pyrone semicarbazone and X = Cl-, Br, I-, NO 3 - and CIO 4 - have been prepared, the ligand and complex have been characterized by elemental analysis IR spectra, electronic spectra, molar mass, conductance, magnetic susceptibility values. DMPS acts as neutral bidentate ligand and coordinate is proposed through azomethine N and carbonyl oxygen atoms of semicarbazone moiety the remaining position of metal ions are satisfied by negative anions such Cl, Br, I, NO 3 or CIO 4 the complexes were proposed to be monomeric octahedral in geometry.

Singh T.,Bra Bihar University | Sinha N.,Bra Bihar University | Singh A.,Mahavir Cancer Sansthan and Research Center
Indian Journal of Pharmacology | Year: 2013

Background: Limited data is available about the toxicity of herbal remedies used for self-medication. Since a popular medicinal plant Ecliptaalba contains various bioactive molecules, the present study aimed to observe the biochemical and histological changes in liver associated with acute oral toxicity (LD 50 ) of aqueous extract of E. alba (L.) Hassk. in female Swiss albino mice. Materials and Methods: For the acute oral toxicity study, the animals were divided into six groups of 6 mice each. Group-I was normal control and the treatment groups were administered aqueous leaf extract of E. alba orally at different doses of 500 mg (group-I),1750 mg (group-III), 2000 mg (group-IV), 2500 mg (group-V) and 3000 mg/kg/b.wt.(group-VI) for seven consecutive days. The mice were sacrificed on the eighth day and blood was collected for the analysis of ALP (alkaline phosphatase), SGPT (serum glutamic pyruvic transferase), total protein and albumin. The liver was dissected, weighed, and processed for histopathological analysis. Results: The LD 50 was found to be 2316.626 mg/kg/body weight in female mice. Serum SGPT, total protein and albumin increased in treated group-IV (P < 0.05), V (P < 0.01), and VI (P < 0.01) as compared to the control (group-I). ALP level significantly decreased in the treated group-IV (P < 0.05), V (P < 0.01) and VI (P < 0.01). Histopathological changes were observed at dose of 2000 mg (group-IV), 2500 mg (group-V) and 3000 mg (group-VI). Conclusion: It was concluded that oral administration of aqueous leaf extract of E. alba had detrimental effects on biochemical parameters and induced histopathological alterations in liver of female Swiss albino mice at doses higher than 2000 mg/kg/day indicating that its indiscriminate use should be avoided.

Prasad P.,T 1 | Bedi S.,Patna University | Singh T.,Bra Bihar University
Malaysian Journal of Microbiology | Year: 2012

Aims: Cellulosic biomass is the only foreseeable sustainable source of fuels and is also one of the dominating waste materials in nature resulting from human activities. Keeping in view the environmental problems like disposal of large volumes of cellulosic wastes and shortage of fossil fuel in the world, the main aim of the present investigation was to characterize and study the cellulolytic activity of Streptomyces albospinus (MTCC 8768), isolated from municipal wastes, on natural cellulosic substrates viz. straw powder, wood powder and finely grated vegetable peels. Methodology and Result: Stanier's Basal broth with 100 mg of each of the substrates was inoculated separately with S. albospinus (MTCC No. 8768) and incubated at 37 oC for 8 days. The cellulosic substrates were re-weighed at an interval of 2 days and the difference between the initial weight and the final weight gave the amount of substrates degraded by the isolate. It was observed that maximum degradation was observed in the grated vegetable peels (64 mg) followed by straw powder (38 mg) and wood powder (28 mg) over a period of 8 days. Conclusion, significance and impact of study: By the selection of efficient cellulolytic microorganisms and costeffective operational techniques, the production of useful end products from the biodegradation of the low cost enormous stock of cellulose in nature can be very beneficial.

Kishore K.R.,Lnt College | Kishore K.R.,Bra Bihar University | Rai B.K.,Lnt College
Asian Journal of Chemistry | Year: 2010

Metal complexes of Co(II), Ni(II) and Cu(II) with Schiff base, 3- amino-2-phenyl-4(3H)quinazoline semicarbazone (APQS) have been synthesized and characterized by elemental analyses, molar mass, molar conductance, magnetic susceptibility, IR, UV-visible studies. These studies proposed tridentate behaviour of the ligand and coordination takes place through azomethine nitrogen, carbonyl oxygen and secondary amino group of quinazoline side chain. Electronic spectra and magnetic susceptibilities value proposes octahedral geometry for the complex. The molar conductance value indicates complexes were electrolytic in nature of the 1:2 type. The ligand APQS and its metal complexes were also screened for their antibacterial and antifungal activities.

Rai B.K.,Bra Bihar University | Rai B.K.,har University Muzaffarpur | Kumar A.,Bra Bihar University | Kumar A.,har University Muzaffarpur
Oriental Journal of Chemistry | Year: 2013

A series of metal complexes derivatives of 1-propyl-2-6-diphenyl piperidone aemicarbazone(PDPS) with metal ions Cu(II), Co(II) and Ni(II) have been synthesized. The ligand and metal complexes obtained are characterized quantitatively and qualitatively by using, molar maaa, elemental analyaea, Infrared apectra, electronic apectra, magnetic auaceptibility and conductivity meaaurementa. On the basis of above phyaiochemical analysia, it has been obaerved that the ligand PDPS coordinate to the metal ion in a bidentate manner through azomethine nitrogen and oxygen atom ot semicarbozone moiety. The remaining coordination centers are satisfied by anions such as X = CV, Br and V. Electronic spectral and magnetic susceptibility measurement proposed the general composition of the complex is [M(PDPS)2X2] where M = Gu(II), X = CV and Br; M = Go(II) and NiOl), X = CV, Br and V. The complexes of 00(11) and NiQI) were proposed octahedral geometries whereas diatorted octahedral geometry reported for CuQI) complexes. The preliminary in vifro antibacterial Screening activity revealed that complexes showed better inhibition against tested bacterial atraina and higher compared to parent ligand.

Kumari M.,Bra Bihar University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Tinospora cordifolia commonly known as Guduchi, is an endangered, medicinal plants found throughout India, used in Ayurvedic rasayans to improve the immune system, memory and mental intelligence. It is also regarded as a liver projector. Due to ever growing demand, the tissue culture techniques have been employed for multiplication and conservation of the important medicinal plant like Tinospora cordifolia. Thus in order to obtain in vitro plants, a protocol was developed for rapid clonal propagation of Tinorpora Cordifolia through nodal explants on Murashige and Skoog medium containing different concentration of growth regulators BAP (1- 2 mg/l) and Kinetin (1- 2 mg/l). The elongated shoots transferred on MS medium supplemented with NAA (0.5- 1.0 mg/l) in order to develop roots thus in vitro grown rooted plants were transferred to sandy soil. The methanolic extract of this plant was found to have antimicrobial activity against Bacillus subtilis (MTCC8), E coli (MTCC1), Staphylococcus aureus (MTCC98), Salmonella typhi (MTCC737). The largest zone of inhibition (18 mm) was found against Staphylococcus aureus. The preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, carbohydrates, and steroids in in vitro grown products like callus, leafyshoots and roots of Tinospora cordifolia.

Usha T.,Bhilai Institute of Technology | Narayan A.,Bhilai Institute of Technology | Ishwar B.,Bra Bihar University
Astrophysics and Space Science | Year: 2014

This paper studies the motion of an infinitesimal mass around triangular equilibrium points in the elliptic restricted three body problem assuming bigger primary as a source of radiation and the smaller one a triaxial rigid body. A practical application of this case could be the study of motion of a satellite under the effect of Sun and Earth. We have exploited the method of averaging used by Grebnikov (Nauka, Moscow, revised 1986) throughout the analysis of stability of the system. The critical mass ratio depends on the radiation pressure, oblateness, eccentricity and semi major axis of the elliptic orbits and the range of stability decreases as the radiation parameter increases. © 2013 Springer Science+Business Media Dordrecht.

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