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Li J.-C.,Bozhou Vocational and Technical College | Huang P.,Anhui University
Xiandai Huagong/Modern Chemical Industry | Year: 2014

An electrochemical detection method is established and used for determination of Pyrolin content. The results show that there is an oxidization peak of Pyrolin in 0.10 mol/L Na2HPO4-NaH2PO4 buffer solution (pH=6.86). The peak current percentile and its concentration have a good linear relationship within the framework of the 4.0×10-7-2.4×10-6 mol/L. Regression equation is: I=-49.7197-1.2872C(r=0.9967). The limit of detection is 4.0×10-7 mol/L. It indicates that this method is easy, reliable and rapid. Source

Liu C.,Nanjing Forestry University | Liu C.,Bozhou Vocational and Technical College | Hu J.,Nanjing Forestry University | Xu H.,Nanjing Forestry University | And 3 more authors.
Chemistry of Natural Compounds | Year: 2015

A facile and efficient one-step synthesis of a novel phenanthroindolizine skeleton was developed via the Vilsmeier–Haack reaction of accessible 18-acetamino-7-oxo-dehydroabietane. The structure and stereochemistry were confirmed by NMR, HR-MS, elemental analysis, and X-ray crystal structure analysis (XSA), and the proposed reaction pathway for the Vilsmeier-Haack reaction includes an unusual conjugate addition of enol to chloromethyleneiminium salt and intramolecular cyclization reaction. © 2015 Springer Science+Business Media New York Source

Huang H.-P.,Anhui University | Huang H.-P.,Chinese Academy of Sciences | Li J.-C.,Bozhou Vocational and Technical College | Huang L.-Q.,Chinese Academy of Sciences | And 3 more authors.
Chinese Journal of Integrative Medicine | Year: 2015

Breeding is not only an important area of medicinal plants research but also the foundation for the superior varieties acquirement of medicinal plants. The rise of modern biotechnology provides good opportunities and new means for medicinal plants breeding research in China. Biotechnology shows its technical advantages and new development prospects in breeding of new medicinal plants varieties with high and stable yield, good quality, as well as stress-resistance. In this paper, we describe recent advances, problems, and development prospects about the application of modern biotechnology in medicinal plants breeding research in China. © 2015, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg. Source

Zhang B.,Bozhou Vocational and Technical College | Zhang D.,Peking University | Meng L.,Beijing Tong Ren Tang Bozhou Traditional Chinese Medicine Ltd.
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2016

In the traceability system of Chinese herbal medicine quality, due to the characteristics of Chinese herbal medicines, we need the shared ownership of radio frequency identification (RFID) tags between the Chinese herbal parties. Because Chinese herbal medicines have unique features as compared to other ordinary commodities, we should put forward higher requirements for the security of RFID tag's data based on the consideration of medicines quality and safety. In the process of the ownership transfer of RFID tags, the transfer protocol must have the advantages of high security, being able to withstand external attacks and prevent leakage of privacy data for real traceability of Chinese herbal medicines. But common ownership transfer protocols are mostly related to the ownership transfer process with a single tag, and have prevalence of privacy data leakage, ownership transfer process instability and other shortcomings. How to achieve the transfer protocol of the shared ownership becomes the emphasis to ensure data security focused on RFID tag, and also becomes the important key to the quality guarantee of Chinese herbal medicines. For the defects of existing ownership transfer protocol with single label, using TTP (trusted third party) symmetric encryption mechanism, we put a new shared ownership transfer protocol i.e. TTP model shared ownership transfer protocol (TSOTP) based on the lightweight encryption algorithm. After the process of initial label certification by the TTP certification authority, we use the symmetric encryption algorithms to produce the symmetric key of generation group. The new owner of the tags uses a shared group key to achieve authentication, and then assigns a new key for the RFID tag which is ultimately authorized; when the new owner of the tags receives the updated label key to confirm the results using hash function operation with the information of the backend server received, then detecting the legality of the shared key, he can read the sensitive data of label. In the implementation of RFID authentication protocol, the calculation of certification and response process will consume a lot of system resources, and how to reduce the amount of calculation becomes a key for the efficiency of protocol operation. After the reader receives the confirmation label, the label will generate a random number by the XOR, the result is encrypted by hash function and to compared with the received information for the previous step, if they get on matching, it will be sent a responsed information to the server, otherwise it will terminate implementation of the agreement in order to avoid the occurrence of the phenomenon of replay attacks. TSOTP protocol can improve the stability of the tab in the transfer process of ownership, well achieve the safe transfer of the shared ownership among users, ensure data security of RFID tags, improve forward and backward security, reduce private data leaks, Dos attacks, replay attacks and other risks, and meanwhile avoid the repeated transfer of ownership, simplify the calculation amount of label certification, which shows good stability and efficiency and meets the research needs of Chinese herbal medicine quality traceability system. Malicious attackers frequently tamper tag data and collect herbs in the transfer process in order to achieve the purpose of obtaining illegal profits. How to improve security of the RFID tags for Chinese herbal's circulation will become the research focus in the future process of medicine traceability system establishment. © 2016, Editorial Department of the Transactions of the Chinese Society of Agricultural Engineering. All right reserved. Source

Huang H.,Anhui University | Gao S.,China Pharmaceutical University | Wang D.,Anhui University | Huang P.,Anhui University | Li J.,Bozhou Vocational and Technical College
Journal of Traditional Chinese Medicine | Year: 2014

OBJECTIVE: To establish and optimize the propagation of Nianmaohuangqin (Radix Scutellariae Viscidulae) and induce and characterize polyploidy of Nianmaohuangqin (Radix Scutellariae Viscidulae). METHODS: Buds from germinating seed-derived explants were induced by tissue culture. With an orthogonal test, different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to determine the optimal concentrations for the propagation of Nianmaohuangqin (Radix Scutellariae Viscidulae). The different concentrations of IAA and rooting powder (ABT) were used to induce rooting. A 0.3% w/v colchicine solution was used to induce polyploidy and the induced buds was identified by root-tip chromosome determination and stomatal apparatus observation. RESULTS: A large number of buds could be induced directly from epicotyl and hypocotyl explants on Murashige and Skoog (MS) (Murashige and Skoog 1962) medium supplemented with 1.1-1.3 mg/L BAP and 0.2 mg/L IAA. Root induction and development could be observed within 20 days of inoculation on 1/2 MS medium supplemented with 0.2 mg/L IAA and 0.1 mg/L ABT. Furthermore, 27 lines of autotetraploid individuals were obtained with a plantlet chromosome number of 2n=4x=36. CONCLUSION: Autotetraploid lines could be obtained through induction with colchicine in vitro, proving that this method might be used for plant selection and breeding. © 2014 JTCM. All rights reserved. Source

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