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Sharma S.,Botany Division Forest Research Institute | Rathi N.,Botany Division Forest Research Institute | Nautiyal S.,Botany Division Forest Research Institute | Arya I.D.,Arid forest Research Institute
Acta Horticulturae | Year: 2010

The present project is taken up with the aim to develop micropropagation protocols for large and commercial scale multiplication of highly important forest trees, i.e., Eucalyptus hybrids. Two Eucalyptus hybrids namely as FRI-5 (Eucalyptus camaldulensis Dehn × Eucalytpus tereticornis Sm.) and FRI-14 (Eucalyptus torelliana F.V. Muell × Eucalyptus citriodora Hook) were selected for the present course of investigation with the aim to develop mass scale micropropagation technique using nodal segments as explants from the mature trees (30-32 years old) of both the hybrids. These Eucalyptus hybrids were produced by Forest Research Institute, Dehradun in the early 1960-70s though controlled crossing are available in very limited number and need multiplication true to type for field-testing to ascertain its superiority under varied agro-climatic conditions. These hybrids showed 3-5 superior in terms of total biomass and wood volume when compared to their parents. Cultures were established and multiplied on MS medium supplemented different concentration of BAP either alone or in with combination of auxins i.e. IBA/NAA. MS medium was found to be effective and suitable for all the experiments during plant production. Eighty-five to 92% rooting was achieved on half strength MS medium supplemented with IBA. Ninety-five to 98% plantlets survival was obtained after hardening and acclimatization prior to field transfer. Parameters like height, DBH, clear bole length and self pruning capability of both the hybrid were recorded up to three years to identify the suitability of these hybrids in different varied climatic zones of Uttarakhand.

Chaukiyal S.P.,Botany Division Forest Research Institute | Mir R.A.,Botany Division Forest Research Institute | Pokhriyal T.C.,Botany Division Forest Research Institute
Journal of Forestry Research | Year: 2013

At the seedling stage, a small amount of N is required to boost growth of leguminous plants. A pot experiment was conducted to observe the effect of N fertilizer on various growth parameters and nodulation behavior of Pongamia pinnata under nursery conditions. After the establishment of seedlings, four nitrogen treatments, 0, 40, 80 and 100 kg·ha-1 N were applied in two equal splits. Monthly observations were taken for the morphological parameters viz. plant height, collar diameter, leaf number, root length, root shoot ratio, nodule number and weight per plant. Maximum plant height was recorded after application of N at 40 kg·ha-1. Seasonally, the difference in collar diameter in rainy season was significantly higher than in winter or summer. However, more leaves were produced per plant at N-40 and N-100 treatments in winter and rainy seasons. Higher root length was recorded in rainy season than in winter or summer. Root biomass was higher than for stems or leaves. Seasonal effects of N-80 and N-40 treatments on leaf dry weight were significantly higher than for N-100 or N-0. Stem dry weight was higher at N-40 than at other treatments in winter and summer seasons. Root:shoot ratio was higher throughout winter to early summer. Nodule biomass was 2-3 times higher in rainy season compared to winter or summer. Maximum nodule number and biomass per plant were highest at N-40, followed by N-0, N-80 and N-100 treatments. New nodule formation started from June to the end of September. Maximum biomass per plant was recorded at N-40, followed by N-80, N-100 and N-0. Nitrogen treatment effect and seasonal behaviour interaction were not significant. Significantly higher numbers of nodules per plant were recorded in rainy season followed by summer and winter for all treatments. Higher nitrogen doses suppressed growth while lower doses promoted growth in Pongamia pinnata. Therefore, the lower nitrogen dose i.e., N-40 Kg·ha-1 applied in two equal splits was suitable at the initial nursery stage for the increase in nodulation and biomass production. © 2013 Northeast Forestry University and Springer-Verlag Berlin Heidelberg.

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