Bor h Food Safety Research Center

Bor Tungge, China

Bor h Food Safety Research Center

Bor Tungge, China

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Xing H.,Shanghai JiaoTong University | Xing H.,Key Laboratory of Urban Agriculture South | Zhan S.,Shanghai JiaoTong University | Zhan S.,Key Laboratory of Urban Agriculture South | And 7 more authors.
RSC Advances | Year: 2013

We report a rapid and facile colorimetric sensing method for melamine detection in milk using aptamer based nanoparticles (AuNPs). Aptamers can absorb on the surface of AuNPs and electrostatically interact with poly(diallyldimethylammonium chloride) (PDDA), which protects the AuNPs from aggregation. The introduction of melamine causes a melamine-aptamer complex to form via hydrogen bonding, thus the resulting cationic polymer can aggregate the AuNPs and cause a remarkable change in color. As a result of this, the presence of melamine can be determined by the naked eye and by measuring absorbance. This sensor is selective for the detection of melamine in milk samples and has a limit of detection of 34 nM. Since aptamer-modified AuNPs are more stable and selective than unmodified AuNPs in the sensing of melamine, we hope that this type of detection method will be helpful for the detection of other small molecules. © 2013 The Royal Society of Chemistry.


He L.,Shanghai JiaoTong University | He L.,Key Laboratory of Urban Agriculture South | He L.,Bor h Food Safety Research Center | Zhi W.,Shanghai JiaoTong University | And 17 more authors.
Analytical Methods | Year: 2012

A sensitive sensor for detection of daunomycin (DNR) was developed by using free-labeled gold nanoparticles (AuNPs) and DNR aptamer. In the absence of DNR, free aptamer could absorb onto the surface of AuNPs to prevent them from aggregating in high salt solution. In the presence of DNR, the aptamer was firstly exhausted due to the formation of a DNR/aptamer complex, which lead to the remarkable change of resonance scattering (RS) intensity at 550 nm. Such sensor showed high selectivity and a detection limit of 17.1 nM. Based on the advantage of the sensor, the proposed sensing method is believed to be implemented to the on-site and real-time DNR detection in food safety and other applications. © 2012 The Royal Society of Chemistry.


Zhong L.,Shanghai JiaoTong University | Zhong L.,Bor h Food Safety Research Center | Wang L.,Shanghai JiaoTong University | Wang L.,Bor h Food Safety Research Center | And 20 more authors.
BioMetals | Year: 2015

Copper (Cu) ion is essential for the biological systems, however, high level of CuCl2 exposure causes detrimental effects, which leads to cell apoptosis. Nitric oxide (NO) is an efficient cell signal messenger, which plays an important role in cell apoptosis. However, the potential mechanism of an early phase Cu-induced acute cytotoxicity through the nitric oxide synthase (NOS) signaling pathway and its interaction has not been studied. In this report, we provide data showing that high level of CuCl2 could rapidly decrease the NO production with the release of Ca2+ and Zn2+, and then modulate the transcriptional and translational expression of NOSs in MCF-7 cells. The reactive oxygen species (ROS) level in cells was increased after high level of CuCl2 exposure, which led to the alpha subunit of eukaryotic initiation factor 2 phosphorylation. By using the free radical scavenger N-acetyl-L-cysteine or the NOS substrate L-arginine, it demonstrated that NOS played a critical role on the Cu-induced ROS generation, which further led to the oxidative stress and cell apoptosis. These results suggested that Cu-induced apoptosis was associated with the oxidative stress, and through the NOS-mediated signaling pathway. © Springer Science+Business Media 2014.


Shi W.-W.,Shanghai JiaoTong University | Shi W.-W.,Bor h Food Safety Research Center | Huang H.-Y.,Shanghai JiaoTong University | Wang N.,Key Laboratory of Urban Agriculture South | And 4 more authors.
Fresenius Environmental Bulletin | Year: 2013

A novel nitrate-uptake bacterial strain Bacillus megaterium NCT-2 was isolated from greenhouse soil. This isolate could utilize nitrate as sole source of nitrogen. The resting cells could remove nitrate over 200 mg/L within 48h. Although nitrate was not essential for the growth of this microorganism, it increased the growth rate. To characterize the microbial nitrate uptake, we measured the effect of sodium chloride and heavy metal ions on the uptake. The increase of sodium chloride concentration inhibited the nitrate removal. When sodium chloride concentration exceed 10%, only very little uptake was observed. And that inhibitory effect of sodium chloride on both nitrate uptake and bacterial growth was dose-dependent. The heavy metal ions, such as Ni 2+, Cd2+, and Cu2+, could inhibit the nitrate uptake. In the opposite case, Zn2+ and Mn2+ had no effects on the nitrate removal. Furthermore, Fe3+can significantly promote the uptake. The effects of metal ions on uptake were consistent with that on the growth of strain NCT-2. This study suggested the strain NCT-2 could be widely used for bioremediation of soil contaminated by nitrate. © by PSP.


Luo Y.,Bor h Food Safety Research Center | He L.,Bor h Food Safety Research Center | He L.,Shanghai JiaoTong University | Zhan S.,Bor h Food Safety Research Center | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

This paper reports an ultrasensitive resonance scattering (RS) method to detect tetracycline (TET) in milk based on the competition of aptamers between nanogold and TET, aggregation of naked nanogold, nanocatalytic Fehling reaction, and RS signals of catalytic product Cu2O cubic. The detection principle was confirmed by the nanoparticle size analyzer (NANOS), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The variations of RS intensity had good linear correlation with TET concentrations, and the limit of detection was calculated as 11.6 nM. The proposed method was successfully applied for analysis of TET in milk, with total recoveries ranging from 105 to 109%. © 2014 American Chemical Society.


He L.,Shanghai JiaoTong University | He L.,Key Laboratory of Urban Agriculture South of Ministry of Agriculture | He L.,Bor h Food Safety Research Center | Luo Y.,Shanghai JiaoTong University | And 8 more authors.
Food Analytical Methods | Year: 2013

We have developed an unlabeled sensing strategy for tetracycline (TET) detection in aqueous solutions and milk by using an aptamer-gold nanoparticle (AuNP)-based colorimetric method. Aptamers were found to be absorbed on the Au surface which stabilizes the AuNPs against conditions of high poly(diallyldimethylammonium) strength. The introduction of TET had reduced aptamers to form a TET-aptamer complex, so that the following cationic polymers could aggregate AuNPs and cause a remarkable change in color. Through this phenomenon, TET can be detected qualitatively and quantitatively by means of absorbance. This unlabeled strategy saves both the time and cost for detection, which could selectively detect TET at concentrations as low as 1 μM and 45.8 nM for the naked eyes and colorimetric detector, respectively. In addition, this biosensor exhibited high selectivity over other antibiotics with an excellent recovery in the detection of milk. Because of the advantages of this sensor, we hope this will be the basis for an assay for the sensitive, colorimetric detection of a wide range of molecular analytes. © 2013 Springer Science+Business Media New York.


Zhong L.,Shanghai JiaoTong University | Zhong L.,Bor h Food Safety Research Center | Wang L.,Shanghai JiaoTong University | Wang L.,Key Laboratory of Urban Agriculture South | And 20 more authors.
Biological Trace Element Research | Year: 2015

Literature to date has confirmed that cadmium (Cd) can accomplish its toxic effects via the free radical-induced damage, but Cd itself cannot generate free radicals directly. Nitric oxide (NO) is a fundamental molecule that interplays with reactive oxygen species (ROS), which may be associated with the Cd-induced cytotoxicity. However, the role of nitric oxide synthase (NOS) in an early phase Cd-induced acute cytotoxicity and its interaction has not been studied. In this report, we provide data showing that CdCl2 (10 μM, 100 μM, 1 mM) could modulate NOS activity in terms of NO production which was first suppressed with the release of Ca2+ and Zn2+, then induced with the transcriptional and translational activation of the three NOS isoforms in a possible feedback manner. The ROS level in cells was increased after CdCl2 exposure. By using the free radical scavenger N-acetyl-l-cysteine (LNAC) or the NOS activity inhibitor NG-methyl-l-arginine (LNMMA), it was demonstrated that NOS played a critical role on the Cd-induced ROS generation. The Cd-induced cytotoxicity was associated with the NOS-mediated oxidative stress in MCF-7 cells. © 2014, Springer Science+Business Media New York.


Zhong L.,Shanghai JiaoTong University | Zhong L.,Bor h Food Safety Research Center | Wang L.,Shanghai JiaoTong University | Wang L.,Bor h Food Safety Research Center | And 20 more authors.
Environmental Toxicology | Year: 2016

The potential Pb-induced cytotoxicity in various tissues and biological systems has been reported. Some evidences also indicate that the Pb-caused cytotoxicity may be associated with the nitric oxide synthase (NOS). However, there remains uncertainty about the role of the NOS signaling pathway during the Pb-induced cytotoxicity. In this report, we provide data showing that PbCl2 treatment depresses the expressions of the three distinct NOS isoforms: neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS) on both transcriptional and translational levels in MCF-7 cells. The down-regulation of NOSs expressions by PbCl2 exposure leads to reduced NOS activity and nitric oxide (NO) production. Meanwhile, the intracellular reactive oxygen species (ROS) level is elevated after PbCl2 exposure, which leads to the alpha subunit of eukaryotic initiation factor 2 (elF2α) phosphorylation. The reduction effects of the free radical scavenger N-acetyl-l-cysteine or the NOS substrate l-arginine on the Pb-induced ROS generation suggest that the NOS signaling pathway plays a key role in the Pb-induced oxidative stress, which further results in the elF2α phosphorylation and cytotoxicity. © 2016 Wiley Periodicals, Inc.


Wu J.,Bor h Food Safety Research Center | Yang C.,Bor h Food Safety Research Center | Rong Y.,Bor h Food Safety Research Center | Wang Z.,Bor h Food Safety Research Center
Procedia Engineering | Year: 2012

In this study, the preparation and trophic characterization of perilla chewable tablet were investigated. Perilla chewable tablet was prepared according to the following process: mixing perilla raw materials with excipients, making wet granules, drying, tabletting and coating. The optimal formula was determined as follows: 8% perilla powder, 2.5% perilla extract powder, 20% isomaltooligosaccharide, 20% microcrystalline cellulose, 44.4% lactose, 0.5% essence of perilla, 0.1% sucralose, 2% erythritol, 2% vitamin C, 0.5% magnesium stearate. Results from nutrient analysis showed that perilla chewable tablet was rich in essential vitamins and mineral substances, which are good for human health. © 2012 Published by Elsevier Ltd.


Wu J.,Bor h Food Safety Research Center | Wu Y.,Bor h Food Safety Research Center | Yang C.,Bor h Food Safety Research Center | Wang Z.,Bor h Food Safety Research Center
Procedia Engineering | Year: 2012

The objective of this study was to investigate the enzymatic preparation method of soybean oligosaccharides (S0S) from okara by using Viscozyme L, characterize the physicochemical properties as well as antioxidant activity of S0S. The effect of Viscozyme L treatment on the preparation of S0S from okara was researched by orthogonal test. Under the fixed extraction time of 2.5 h and the ratio of okara to water of 1:15 (w/v), the optimal enzymatic preparation conditions of S0S by using Viscozyme L were identified as pH of 3.5, temperature of 45 °C, enzyme dosage of 3% (w/v) with a maximum yield of 10% (w/w). S0S were found to be crude oligosaccharides containing 56.5% neutral sugar, having a low molecular weight distribution range between 860 and 9380 Da, composing of galactose, xylose, rhamnose, arabinose, mannose and glucose. Furthermore, S0S showed the free radical scavenging activity with IC50 value of 1.81 mg/mL. These results suggest that S0S are novel oligosaccharides derived from okara, and could be improve value-added utilization of okara as antioxidants in functional foods and nutraceuticals. © 2012 Published by Elsevier Ltd.

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