BONAC Corporation

Fukuoka-shi, Japan

BONAC Corporation

Fukuoka-shi, Japan

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Patent
Bonac Corporation | Date: 2015-10-29

Provided is a novel nucleic acid molecule that is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes, in sequence from the 5 side to the 3 side: a 5 side region (Xc); an inner region (Z); and a 3 side region (Yc). The inner region (Z) is composed of an inner 5 side region (X) and an inner 3 side region (Y) that are linked to each other. The 5 side region (Xc) is complementary to the inner 5 side region (X). The 3 side region (Yc) is complementary to the inner 3 side region (Y). At least one of the inner region (Z), the 5 side region (Xc), and the 3 side region (Yc) includes the expression inhibitory sequence.


Patent
Bonac Corporation | Date: 2016-06-30

The invention provides a microRNA inhibitor that has two or more sequences complementary to the sequence of microRNA to be the target of inhibition, which two or more complementary sequences are linked via one or more linker residues.


Patent
Bonac Corporation | Date: 2015-01-14

Provided is a microRNA inhibitor having broad utility and difficult to degrade. A microRNA inhibitor 1 characterized in that it has two or more sequences 2 complementary to the sequence of microRNA to be the target of inhibition, and the two or more complementary sequences 2 are linked via linker residue(s) 3.


Patent
Bonac Corporation and Tokyo Medical University | Date: 2016-11-02

The present invention provides a new artificial match-type miRNA utilizing miRNA. A single strand nucleic acid containing X region and Y region, wherein the 3-terminal of the X region and the 5-terminal of the Y region are linked via a linker region of a non-nucleotide structure, the X region contains a guide strand sequence of a mature miRNA, and the Y region contains a sequence completely complementary to the X region is an artificial match-type miRNA. The artificial match-type miRNA can suppress expression of the target gene.


Patent
Bonac Corporation | Date: 2014-04-23

The present invention provides a process for preparing an isotope-containing phosphate compound easily. The process for preparing an isotope-containing phosphate compound according to the present invention includes the step of: oxidizing a trivalent phosphorus compound with an oxidizing agent containing an isotope to synthesize a pentavalent phosphate compound to which the isotope has been introduced. The present invention preferably is applied to the synthesis of nucleic acids such as DNA and RNA, for example. The isotope preferably is a stable isotope. The oxidizing agent preferably is H_(2)^(18)O, 3H-1,2-benzodithiol 3-one 1,1-dioxide having ^(34)S, or a diisopropylethylamine-borane complex having ^(10)B, for example.


Patent
Bonac Corporation | Date: 2014-11-12

The present invention aims to provide a novel nucleic acid molecule that can be produced easily and efficiently, and can inhibit gene expression. Provided is a single-stranded nucleic acid molecule containing an expression inhibitory sequence that inhibits expression of a target gene, and containing region (X), linker region (Lx) and region (Xc), wherein the linker region (Lx) is linked between the region (Xc) and the region (Xc), the region (Xc) is complementary to the region (X), at least one of the region (X) and the region (Xc) contains the expression inhibitory sequence, and the linker region (Lx) contains an atomic group derived from an amino acid. The single-stranded nucleic acid molecule can inhibit expression of the above target gene.


Patent
Bonac Corporation | Date: 2015-10-21

Provided is a novel nucleic acid molecule capable that can inhibit the expression of a gene and can be produces easily and efficiently. The nucleic acid molecule contains is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes, in sequence from the 5 side to the 3 side: a 5 side region (Xc); an inner region (Z); and a 3 side region (Yc). The inner region (Z) is composed of an inner 5 side region (Xc) and an inner 3 side region (Y) that are linked to each other. The 5 side region (Xc) is complementary to the inner 5 side region (X). The 3 side region (Yc) is complementary to the inner 3 side region (Y). A linker region (Lx) is present between the 5 side region (Xc) and the inner 5 side region (X). The 5 side region (Xc) and the inner 5 side region (X) are linked to each other via the linker region (Lx). The linker region (Lx) comprises a non-nucleotide residue. At least one of the inner region (Z), the 5 side region (Xc), and the 3 side region (Yc) includes the expression inhibitory sequence. According to this single-strand nucleic acid molecule, it is possible to inhibit the expression of the target gene.


An ether is provided which may be used in the production of a glycoside, and method of producing the ether. The ether is represented by the following chemical formula (106):R^(4) is a hydrocarbon group, a straight chain or branched alkyl group, a straight chain or branched alkenyl group, a straight chain or branched alkynyl group, an aryl group, a straight chain or branched arylalkyl group, a cycloalkyl group, a cycloalkenyl group, a straight chain or branched cycloalkylalkyl group, a straight chain or branched cyclylalkyl group, or a straight chain or branched alkoxyalkyl group,n is a positive integer,L^(1) is an ethylene group (-CH_(2)CH_(2)-), wherein hydrogen atoms besides a hydrogen atom bonded to the -position relative to [D^(1)] are optionally substituted by a straight chain or branched alkyl group, and[D^(1)] is an electron-withdrawing group.


The invention provides a glucoside compound, which is capable of providing a phosphoramidite, which can be produced at low cost and can produce a nucleic acid in high yield and with high purity. The glycoside compound has the formula wherein B, R1, R2, and R3 are as described herein.


Provided is a single-stranded nucleic acid capable of inhibiting expression of a target gene having a delivery function. A molecule containing, from the 5-side to the 3-side, a 5-side region (Xc), a linker region (Lx), an inner region (Z), a linker region (Ly) and a 3-side region (Yc) in this order, whereinthe aforementioned inner region (Z) is constituted by linkage of the inner 5-side region (X) and the inner 3-side region (Y),the aforementioned 5-side region (Xc) is complementary to the aforementioned inner 5-side region (X),the aforementioned 3-side region (Yc) is complementary to the aforementioned inner 3-side region (Y),at least one of the aforementioned inner region (Z), the aforementioned 5-side region (Xc) and the aforementioned 3-side region (Yc) comprises an expression inhibitory sequence that inhibits expression of a target gene, andat least one selected from the group consisting of the 5-terminus, the 3-terminus, the aforementioned linker region (Lx) and the aforementioned linker region (Ly) is bound to a bio-related substance.

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