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Lemche A.V.,Charité - Medical University of Berlin | Chaban O.S.,Bogomolets National Medical University | Lemche E.,King's College London
Psychiatry Research | Year: 2014

Alexithymia is a clinical trait consisting of diminished introspective and interoceptive capacities that has been shown to implicate elevated autonomic outflow and to bias for hypertension. To estimate relative risk associated with alexithymia in the metabolic syndrome (MetS), we conducted a cross-sectional analysis of patients with manifest type 2 diabetes mellitus (T2DM) or familial diabetes risk (N=101; 67 females; age 45.6±13.96) in a nationwide sampled treatment cohort for MetS in the Ukrainian governmental health care system. Laboratory data of single components of the MetS according to International Diabetes Federation Consensus were dependent measures in multivariable regression models with self-reported alexithymia severity (TAS-20) and socio-demographic data. TAS-20 as the sole surviving psychometric predictor for T2DM in the simplest regression equation provided the best model fit: OR 1.073, Z=19.04, (95%CIs 1.065-1.081). For microalbuminuria, the best fitting model was OR 1.030, Z=3.49 (95%CIs 1.013-1.048). TAS-20 predicted also triglyceride level at Wald-χ2=1299.27, Z=36.05 (95%CIs 0.052-0.058) and blood pressure maximum at Wald-χ2=2309.05, Z=48.05 (95%CIs 2.402-2.606). Our results show that alexithymia severity contributes to MetS by covarying with several of its single components, and that it may be a substantial concurrent indicator of T2DM and cardiovascular risks in MetS. © 2013 Elsevier Ireland Ltd.

Abenavoli L.,University of Catanzaro | Masarone M.,University of Salerno | Peta V.,University of Catanzaro | Milic N.,University of Novi Sad | And 3 more authors.
World Journal of Gastroenterology | Year: 2014

Hepatitis C virus (HCV) infection is a common chronic liver disease worldwide. Non-alcoholic fatty liver disease and insulin resistance (IR) are the major determinants of fibrosis progression and response to antiviral therapy. The pathogenetic link between IR and chronic HCV infection is complex, and is associated with HCV genotype. Liver steatosis is the most common in the patients infected with genotype 3 virus, possibly due to direct effects of genotype 3 viral proteins. To the contrary, hepatic steatosis in the patients infected with other genotypes is thought to be mostly due to the changes in host metabolism, involving IR. In HCV genotype 3, liver steatosis correlates with viral load, reverts after reaching the sustained virologic response and reoccurs in the relapsers. A therapeutic strategy to improve IR and liver steatosis and subsequently the response to antiviral treatment in these patients is warranted. © 2014 Baishideng Publishing Group Inc. All rights reserved.

Shatursky O.Ya.,Palladin Institute of Biochemistry | Romanenko O.V.,Bogomolets National Medical University | Himmelreich N.H.,Palladin Institute of Biochemistry
Journal of Membrane Biology | Year: 2014

The action of antifungal drug, amphotericin B (AmB), on solvent-containing planar lipid bilayers made of sterols (cholesterol, ergosterol) and synthetic C14-C18 tail phospholipids (PCs) or egg PC has been investigated in a voltage-clamp mode. Within the range of PCs tested, a similar increase was achieved in the lifetime of one-sided AmB channels in cholesterol- and ergosterol-containing membranes with the C16 tail PC, DPhPC at sterol/DPhPC molar ratio ≤1. The AmB channel lifetimes decreased only at sterol/DPhPC molar ratio >1 that occurred with sterol/PC molar ratio of target cell membranes at a pathological state. These data obtained on bilayer membranes two times thicker than one-sided AmB channel length are consistent with the accepted AmB pore-forming mechanism, which is associated with membrane thinning around AmB-sterol complex in the lipid rafts. Our results show that AmB can create cytotoxic (long open) channels in cholesterol membrane with C14-C16 tail PCs and nontoxic (short open) channels with C17-C18 tail PCs as the lifetime of one-sided AmB channel depends on ∼2-5 Å difference in the thickness of sterol-containing C16 and C18 tail PC membranes. The reduction in toxic AmB channels efficacy can be required at the drug administration because C16 tails in native membrane PCs occur almost as often as C18 tails. The comparative analysis of AmB channel blocking by tetraethylammonium chloride, tetramethylammonium chloride and thiazole derivative of vitamin B1, 3-decyloxycarbonylmethyl-4-methyl-5-(2-hydroxyethyl) thiazole chloride (DMHT), has proved that DMHT is a comparable substitute for both tetraalkylammonia that exhibits a much higher affinity. © 2014 Springer Science+Business Media.

Kobyliak N.,Bogomolets National Medical University | Abenavoli L.,University of Catanzaro
Reviews on Recent Clinical Trials | Year: 2014

Liver biopsy, since 1883, when were first performed, became the gold standard to confirm the earlier stages of fibrosis and grading of non-alcoholic fatty liver disease (NAFLD) and for distinguishing simple steatosis from nonalcoholic steatohepatitis (NASH). General limitations of liver biopsy are sampling error and interand intraobserver variability. Also procedure is invasive and that’s why associated with some potential adverse effects and complications which may be minor (pain or vagal reactions, transient hypotension) or major such as visceral perforation, bile peritonitis or significant bleeding. Presence of steatosis, hepatocellular injury in the form of ballooning, lobular inflammation and perisinusoidal fibrosis, usually with a zone 3 distribution are considered to be most important histological features of adult NAFLD which may differ from bariatric surgery or pediatric patients. In addition, grading and staging and current semiquantitative systems for NAFLD assessment are discussed. © 2014 Bentham Science Publishers.

Gorpinchenko I.,Ukrainian Academy of Sciences | Nikitin O.,Bogomolets National Medical University | Banyra O.,2nd Lviv Municipal Polyclinic | Shulyak A.,Ukrainian Academy of Sciences
Central European Journal of Urology | Year: 2014

Introduction It is impossible to imagine a modern socially-active man who does not use mobile devices and/or computers with Wi-Fi function. The effect of mobile phone radiation on male fertility is the subject of recent interest and investigations. The aim of this study was to investigate the direct in vitro influence of mobile phone radiation on sperm DNA fragmentation and motility parameters in healthy subjects with normozoospermia. Material and methods 32 healthy men with normal semen parameters were selected for the study. Each sperm sample was divided into two equal portions (A and B). Portions A of all involved men were placed for 5 hours in a thermostat, and portions B were placed into a second thermostat for the same period of time, where a mobile phone in standby/talk mode was placed. After 5 hours of incubation the sperm samples from both thermostats were re-evaluated regarding basic motility parameters. The presence of DNA fragmentation in both A and B portions of each sample was determined each hour using a standard sperm chromatin dispersion test. Results The number of spermatozoa with progressive movement in the group, influenced by electromagnetic radiation, is statistically lower than the number of spermatozoa with progressive movement in the group under no effect of the mobile phone. The number of non-progressive movement spermatozoa was significantly higher in the group, which was influenced by cell phone radiation. The DNA fragmentation was also significantly higher in this group. Conclusions A correlation exists between mobile phone radiation exposure, DNA-fragmentation level and decreased sperm motility.

Tryliskyy Y.,Bogomolets National Medical University
Travel Medicine and Infectious Disease | Year: 2011

This study was undertaken to assess the duration of altitude acclimatization retention in individuals after initial exposure to a maximum altitude of 5360 m during a mountaineering expedition. Spectral heart rate variability analysis accompanied by an assessment of acute mountain sickness using the Lake Louise Scoring System was performed during two sequential mountaineering expeditions to altitudes of 5360 m and 5642 m, with a period of 30 days between each expedition. Subjects displayed varying degrees of alterations in heart rate variability during the initial expedition, which indicated differing degrees of dysadaptation and stress development. Their Lake Louise Scores accounted for the presence of acute mountain sickness throughout the trip. During the subsequent expedition, the subjects' heart rate variability measures were within the normal range, and there were no signs of acute mountain sickness. All three subjects who underwent step-by-step exposure to altitudes of 5360 m displayed differing degrees of alterations in heart rate variability in conjunction with differing degrees of acute mountain sickness. All subjects also developed acclimatization to hypoxic conditions at this altitude, which was preserved for 30 days, and was sufficient to prevent them from showing any significant alterations in heart rate variability when re-exposed to the same altitude. © 2011 Elsevier Ltd. All rights reserved.

Chalyi A.V.,Bogomolets National Medical University
Springer Proceedings in Physics | Year: 2015

Problem of finding the pair correlation function G2 and correlation length ξ of order parameter fluctuations in liquids in restricted geometry is discussed. Two types of dimensional crossover (DC) are studied. The 1st type (DC-1) corresponds to transition from 3D bulk to 3D bounded liquids. In this case the dependence of physical properties on thermodynamic variables (temperature, density, pressure, etc.) in bulk liquids with linear sizes L ≫ ξ may convert into dependence of these properties on linear sizes in bounded liquids with L < ξ. The 2nd type (DC-2) corresponds to the case when a further decreasing of linear sizes in confined liquids may be treated under certain conditions as the change of spatial dimensionality D (for example, 3D ⇔ 2D crossover in slit-like pores or 3D ⇔ 1D crossover in cylindrical pores). Smooth transition of effective critical exponents (say, from ν = 0.625 for D = 3 to ν = 1 for D = 2) as well as theoretical results versus experimental data are examined for liquids in reduced geometry. © Springer International Publishing Switzerland 2015.

Kugler T.,Bogomolets National Medical University
The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi | Year: 2015

METHODS: A total of 120 FD patients that met the Rome III criteria and 30 healthy volunteers were included in this cross-sectional study. The mean cross-sectional area of the fornix was measured to investigate fundic accommodation and gastric emptying during and after water intake. During the test, abdominal symptoms were evaluated using the 4-point Likert scale.BACKGROUND/AIMS: The major causes of functional dyspepsia (FD) are motility dysfunction and visceral hypersensitivity. Despite the large number of diagnostic tests, there are no convenient methods for evaluation of gastric functions. Therefore, this study was conducted to assess the relationship between the degree of dyspepsia and gastric accommodation, emptying, and sensitivity.RESULTS: The water-drinking ultrasonography combined test revealed impairment of gastric accommodation in FD after 1,000 mL of water intake, delayed emptying after 5 min of water intake and statistically significant hyperesthesia after 400 mL of water intake in the FD group compared with healthy controls (p <œœ 0.05). Postprandial distress syndrome (PDS) and overlap syndrome were independently associated with gut motor disturbances instead of epigastric pain syndrome (EPS) (p <œœ 0.01).CONCLUSIONS: The results of the present study suggest that the water-drinking ultrasonography combined test could be used for diagnosis of gastric motor and sensory dysfunction, particularly in PDS and EPS-PDS patients. This test is easy, well tolerated by the patient and can be widely applied in clinical practice.

Birsan I.,Bogomolets National Medical University | Birsan I.,Apt Therapeutics, Inc.
European Archives of Paediatric Dentistry | Year: 2014

Aim: This study was to determine the markers representative of pathogenic periodontal microflora [Prevotella intermedia (P.i), Tannerella forsythia (T.f) [Bacteroides forsythus], Treponema denticola (T.d), Actinobacillus actinomycetemcomitans (A.a), Porphyromonas gingivalis (P.g)] in the dental plaque of adolescents with various degrees of severity of periodontium inflammation. Methods: Forty-patients aged 15–16 years of age were examined using PMA, CPI and Green–Vermillion indices (Müller 2001). The hygiene status of each patient was also determined using Durr Dental’s Vista Proof intraoral camera (Germany). The polymerase chain reaction (PCR) was performed using a Biometra Thermocycler to detect DNA of pathogenic periodontal bacteria in dental plaque. Results: All marker microorganisms (P.i. + T.f. + T.d. + A.a. + P.g.) were identified in patients diagnosed with periodontitis in dental plaque. A direct correlation between the level of oral hygiene and the severity of the pathological process in it was determined. It was found that the increase in the severity of the disease was accompanied by increased pathogenic periodontal microflora in dental plaque. Conclusion: Identification of periodontal pathogens in dental plaque by PCR greatly enhances the early diagnosis of Cronic cattaral gingivitis (CCG) risk factors in adolescents, and allows for detailed analysis of the relation between each factor and severity of the process. This method may be used for the diagnosis of periodontal diseases, prediction of their future course, and reasonable choice of antimicrobial therapy. © 2014, European Academy of Paediatric Dentistry.

Sakkas C.,Private Paediatric Dental Clinic | Khomenko L.,Bogomolets National Medical University | Trachuk I.,Bogomolets National Medical University
European Archives of Paediatric Dentistry | Year: 2013

Aim: This prospective clinical study evaluated the sealant retention rate and caries-preventive efficacy of two fissure sealing techniques over a 3-year period. Methods: Subjects (87) aged between 6. 5 and 11. 5 years were enrolled into two groups. In Group I (41 subjects), a resin-based fissure sealant (Fissurit FX, VOCO, Germany) was placed on all four sound permanent first molars (FPM-164 teeth) of each subject and in Group II (46 subjects) on 4 caries-free premolars (P1-2 182 teeth),using the following adhesives systems: Optibond FL (Kerr, USA), Optibond Solo plus (Kerr, USA), Prompt-L-Pop (3 M ESPE, USA) or the conventional acid-etch technique. Sealed teeth were evaluated at baseline, 6, 12, 18, 24 and 36-month intervals with regard to retention and new caries development. Sealant retention was scored as: (1) complete retention; (2) partial loss; (3) complete loss of sealant. Statistics: Wilcoxon-signed rank test was used to analyse the data. Results: The complete retention rate was: Optibond FL/sealant 80. 01-82. 92 %, Optibond Solo plus/sealant 73. 78-74. 27 %, Prompt-L-Pop/sealant 42. 84-53. 65 % versus acid-etch technique 62. 86-71. 44 % on FPM and P1-2 (p < 0. 05). The fissure caries incidence rate was: Optibond FL/sealant 2. 43-2. 85 %, Optibond Solo plus/sealant 2. 85-4. 76 %, Prompt-L-Pop/sealant 24. 40-34. 28 % versus acid-etch technique 16. 66-17. 14 % (p < 0. 05) on FPM and P1-2. A statistically significant difference in sealant retention rate was observed between FPM and P1-2, using the self-etching adhesive system Prompt-L-Pop and acid-etch technique. Conclusion: It may be concluded that the use of adhesive systems Optibond FL and Optibond Solo plus yielded better fissure sealing performance. © 2013 European Academy of Paediatric Dentistry.

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