Qingzhou, China
Qingzhou, China

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Yang W.,Huazhong Agricultural University | Tang K.,Huazhong Agricultural University | Zhang C.,Huazhong Agricultural University | Xu D.,Boer Goat Breeding Station | And 2 more authors.
South African Journal of Animal Sciences | Year: 2011

The polymorphism in the gonadotropin-releasing hormone receptor (GnRHR) gene was detected by PCR single-strand conformational (PCR-SSCP) and DNA sequencing methods in 224 Boer goats. Two single nucleotide polymorphisms (SNPs), G891T and G757A, were found in the exon I of the goat GnRHR gene and their associations with litter size in Boer goats were evaluated. Association analysis showed that G891T and G757A had significant effects on litter size. In locus G891T, individuals with GT genotype had significantly larger litter sizes compared to GG genotype in the first and third parity. In locus G757A, ewes with GG genotype had significantly higher litter sizes than those of GA in the fourth parity. The present study primarily revealed an association between allele T at 891 locus of the GnRHR gene and high litter size in the first and third parity in Boer goats. These results provide evidence that the GnRHR gene may be significantly correlated with a high prolificacy in goats.


Zhang C.Y.,Huazhong Agricultural University | Wu C.J.,Huazhong Agricultural University | Zeng W.B.,Huazhong Agricultural University | Huang K.K.,Huazhong Agricultural University | And 7 more authors.
Small Ruminant Research | Year: 2011

The polymorphism in follicle stimulating hormone beta (FSHB) subunit gene was detected by PCR-SSCP and PCR-RFLP methods in 780 does from four goat breeds including Boer, Matou, Black and Boer-Matou crossbred (BM). The associations of FSHB genotypes with litter size (LS), litter weight at birth (LWB) and gestation length (GL) as well as superovulation performances were analyzed in relatively lower-prolific Boer and higher-prolific Matou breeds. A 1514. bp linear DNA sequence of goat FSHB gene covering the complete 3 exons was cloned by four pairs of primer. A new mutation (A2645G, GenBank Accession no: S64745) locating in exon 3 causing an amino acid change from glutamine (Gln) to arginine (Arg) at residue 115 was identified, which resulted in three genotypes named AA, AB and BB. The three genotypes were detected in the four studied goat breeds. The higher-prolific Matou breed had the highest frequency of genotype AA. Association analysis showed that Boer and Matou does with AA genotype have the largest LS both in average and in parities from first to fourth (P< 0.05). Furthermore, Boer does with AA genotype had the heaviest LWB compared to other two genotypes (P< 0.05). Matou does with AA genotype have more numbers of ova harvested, large follicles and corpus lutea on ovaries after superovulation than those with BB genotype (P< 0.05). Therefore, these results suggest that the FSHB gene is a candidate gene that affects reproduction traits in goats. © 2010.


Zhang C.,Huazhong Agricultural University | Liu Y.,Huazhong Agricultural University | Huang K.,Huazhong Agricultural University | Zeng W.,Huazhong Agricultural University | And 3 more authors.
Genetics and Molecular Biology | Year: 2011

Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p < 0.05). On combining the two loci, both Matou and Boer dams with ABCD genotype had the largest litter sizes when compared to the other genotypes (p < 0.05). When undergoing like superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds. © 2001, Sociedade Brasileira de Genética.


Zhang C.,University of Alberta | Liu Y.,Huazhong Agricultural University | Xu D.,Boer Goat Breeding Station | Wen Q.,Shiyan Municipal Bureau of Animal Husbandry | And 3 more authors.
Molecular Biology Reports | Year: 2012

Abstract Polymorphisms of myostatin (MSTN) gene were investigated as a candidate marker for goat growth in 687 individuals by gene sequencing and polymerase chain reaction restriction fragment length polymorphism methods. Three potential genotypes (AA, AB and BB) of 5 bp indel (1,256 TTTTA/-) in 50UTR were detected in four breeds. The polymorphism (CC, CD and DD) of substitution (1,388 T/A) in exon 1 was only segregating in Boer. Genotype AB resulted in significant increases in body weights at birth (BW0), 90 (BW90) and 300 (BW300) days of age, and birth body length compared to BB (P<0.05). Whilst genotype CD contributed to heavier BW0 and BW90, and larger birth body height (BH) compared to CC (P<0.05). Individuals either with AB or CD genotype also had greater values in other body sizes, although no significant differences appeared (P[0.05). When in combination, the combined genotype ABCD displayed positive impacts on better growth traits in BW0, BW90, BW300, BH and BCG (P<0.05). These results indicate that these markers in myostatin (MSTN) are associated with Boer growth and may be useful for marker assisted selection. © Springer Science+Business Media B.V. 2011.

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