Bode Technology Group Inc.

King and Queen Court House, VA, United States

Bode Technology Group Inc.

King and Queen Court House, VA, United States

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Frank E.M.,University of Tennessee at Knoxville | Mundorff A.Z.,University of Tennessee at Knoxville | Davoren J.M.,Bode Technology Group Inc.
Forensic Science International | Year: 2015

Identifying human remains often begins with cleaning and imaging the material. Hot water maceration is used to remove adherent soft tissue from bone and radiographs are taken to better visualize osseous details. Heat and radiation are known to have harmful effects on DNA, but their ability to degrade DNA when used for cleaning and imaging has not been well studied. To better understand their individual and combined effects on the recoverability of DNA from bone, skeletal samples were subjected to (1) hot water maceration (62°C for 45min); (2) CT scanning (0.6mm slices, 120kV, 10.4s); (3) X-ray (50kVp, 150mA, 0.03s, 40in); and (4) all 3 treatments combined. Forty-eight DNA samples were extracted, quantified and amplified with the AmpFLSTR® Identifiler® system. Nearly all of the processed samples had reduced RFU values relative to the unprocessed samples, indicating some amount of genetic loss. This loss did not always translate into loss of profile completeness, since only a few samples had a reduction in the number of loci detected after processing. DNA yields were not significantly reduced by any one of the processing methods, however the results indicate that the damaging effects are additive. It is possible that processing may reduce a bone's DNA reservoir and as more procedures are preformed, the pool of available genetic information might be diminished. Many intrinsic and extrinsic factors can affect the recoverability of DNA from bone. Collecting a DNA sample prior to processing avoids the negative effects from hot water maceration and radiological imaging. © 2015 Elsevier Ireland Ltd.


PubMed | University of Tennessee at Knoxville and Bode Technology Group Inc.
Type: | Journal: Forensic science international | Year: 2015

Identifying human remains often begins with cleaning and imaging the material. Hot water maceration is used to remove adherent soft tissue from bone and radiographs are taken to better visualize osseous details. Heat and radiation are known to have harmful effects on DNA, but their ability to degrade DNA when used for cleaning and imaging has not been well studied. To better understand their individual and combined effects on the recoverability of DNA from bone, skeletal samples were subjected to (1) hot water maceration (62 C for 45 min); (2) CT scanning (0.6mm slices, 120 kV, 10.4s); (3) X-ray (50 kVp, 150 mA, 0.03 s, 40 in); and (4) all 3 treatments combined. Forty-eight DNA samples were extracted, quantified and amplified with the AmpFLSTR() Identifiler() system. Nearly all of the processed samples had reduced RFU values relative to the unprocessed samples, indicating some amount of genetic loss. This loss did not always translate into loss of profile completeness, since only a few samples had a reduction in the number of loci detected after processing. DNA yields were not significantly reduced by any one of the processing methods, however the results indicate that the damaging effects are additive. It is possible that processing may reduce a bones DNA reservoir and as more procedures are preformed, the pool of available genetic information might be diminished. Many intrinsic and extrinsic factors can affect the recoverability of DNA from bone. Collecting a DNA sample prior to processing avoids the negative effects from hot water maceration and radiological imaging.


Patent
Bode Technology Group Inc. and Sangha | Date: 2013-07-03

A track follower (1226a, b) extending from said cover for insertion into said longitudinal track to provide slidable moveable of said cover within said track, said track follower having a frictional fit in said track to provide frictional resistance to the movement of said slidable cover along said track for retention of said cover in any operator selected position when said handle is removed from said holder, and opposed outside cover edges for frictional contact with said opposed flanges (1218a, 1218b) for frictional resistance to the movement of said cover on handle when said handle is within said holder for the retention of said cover in any operator selected position, such that said handle is releasable from said holder for dipping said collection absorbent into liquid specimen.

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