BMS College for Women

Basavanagudi, India

BMS College for Women

Basavanagudi, India

Time filter

Source Type

Tejavathi D.H.,Bangalore University | Devaraj V.R.,Bangalore University | Murthy S.M.,Bangalore University | Anitha P.,BMS College for Women | Nijagunaiah R.,Bangalore University
Indian Journal of Biotechnology | Year: 2010

An effective protocol for the regeneration of multiple shoots from the callus, derived from shoot tip and cotyledonary node, was developed for the first time for Macrotyloma uniflorum (Lam.) Verdc. Shoot apices and cotyledonary nodes from 9-d-old aseptically grown seedlings were inoculated onto MS, L 2 and MMS media supplemented with various growth regulators. Proliferation of about 17 shoots was obtained on AS supplemented MS medium after 16 d of culture, while about 14 and 20 shoots regenerated from the callus on MMS1+NAA (1.86 μM)+BAP(0.45 μM) and L2+IBA (0.45 μM)+BAP (0.11 μM), respectively after 30 d of culture. Regenerated shoots were rooted on MS+IAA (0.11 μM)+Kn (0.21 μ) with upto 6 roots per shoot. Rooted shoots were sequentially acclimatized by transferring them to sterile distilled water and tap water before planting in various pot mixtures. A maximum of 60% survival rate was noticed on a mixture of soil:sand:manure (1:1:1).


Tejavathi D.H.,Bangalore University | Manjula B.L.,Bangalore University | Anitha P.,BMS College for Women
Acta Horticulturae | Year: 2010

The alkaloids present in Adhatoda vasica Nees. namely vasicine and vasicinone are responsible for its pharmacological property. Due to exploitation of the plant for various medicinal uses, there is a need to conserve this plant. High frequency and maximum number of multiple shoots were obtained from shoot tip explants harvested during the month of May on MS medium supplemented with BAP (22.20 μm). Rest of the year the explants were turning brown or black after a day of inoculation because of phenolic substances present in the explant. High frequency of rooting was recorded on MS basal medium. The rooted plantlets were hardened and established at 50-60%.


Nanda N.,Bms College For Women | Dakshayani S.,Bms College For Women | Puttaswamy,Bangalore University
Oxidation Communications | Year: 2011

The kinetics of oxidation of ciprofloxacin (CPF) by sodium N-chlorobenzenesulphonamide or chloramine-B (CAB) in HCI medium at 30 °C shows first order dependence each on oxidant, substrate and H + ion concentrations, but is independent of [Cl -]. The reaction is subjected to changes in: (a) ionic strength; (b) dielectric constant, and (c) added reaction product, and the subsequent effects on the reaction rate are studied. The reaction was studied at different temperatures, and activation parameters have been computed for the overall reaction from the Arrhenius plot. Oxidation products were identified by TLC and spectral (IR and NMR) analysis. A mechanism involving interaction of conjugate acid (PhSO 2NHCl) and substrate giving an intermediate complex in a slow step has been suggested. The proposed mechanism and the derived rate law are consistent with the observed kinetics.


Nanda N.,Bms College For Women
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

Kinetics and mechanism of Sulfaquinoxaline [2-(p-aminobenzene) sulfonamide quinoxaline],(SQL) by Chloramine-B (CAB) in alkaline buffer solution of pH 9.2 over the temperature range of 293-323K has been studied. The reaction is first order with respect to [CAB] and fractional order with respect to each [SQL] and [OH]. The dependence of reaction rate on dielectric constant of the medium and ionic strength is consistent with the proposed mechanism. The observed retardation of rate by addition of reaction product, benzenesulfonamide indicated the involvement of OCl- as the oxidizing species. Activation parameters are evaluated. Rate equation is derived to account for the observed kinetic data and the proposed reaction mechanism. The oxidation products are identified and characterized by spectral data. © 2010 RJPBCS.


Nanda N.,Bms College For Women | Mayanna S.M.,Bms College For Women
Oxidation Communications | Year: 2013

The kinetics of oxidation of norfloxacin (NRF) by sodium N-chlorobenzene sulphonamide (Chloramine-B, CAB) has been studied in aqueous hydrochloric acid medium at 303 K. The reaction is first order with respect to [CAB] and [HCl], fractional order on [NRF]. Activation parameters were evaluated from the kinetic data at different temperatures. A negative entropy of activation indicated the involvement of a rigid complex in the activated state. The dielectric constant of the medium has a small effect on the rate. Ionic strength and the reaction product benzenesulphonamide have no effect on the reaction rate. The solvent isotope effect is studied. The reaction products are identified by spectral (IR and NMR) data. Rate equation is derived to account for the observed kinetic data and a probable mechanism has been proposed.

Loading BMS College for Women collaborators
Loading BMS College for Women collaborators