Saitama, Japan
Saitama, Japan

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Miyoshi-Akiyama T.,National Center for Global Health and Medicine | Hayakawa K.,U.S. Center for Disease Control and Prevention | Ohmagari N.,U.S. Center for Disease Control and Prevention | Shimojima M.,BML Inc. | Kirikae T.,National Center for Global Health and Medicine
PLoS ONE | Year: 2013

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study. © 2013 Miyoshi-Akiyama et al.


Tada T.,Japan National Institute of Infectious Diseases | Miyoshi-Akiyama T.,Japan National Institute of Infectious Diseases | Shimada K.,Japan National Institute of Infectious Diseases | Shimojima M.,BML Inc. | Kirikae T.,Japan National Institute of Infectious Diseases
Antimicrobial Agents and Chemotherapy | Year: 2013

Pseudomonas aeruginosa NCGM1588 has a novel chromosomal class 1 integron, In151, which includes the aac(6′)-Iaj gene. The encoded protein, AAC(6′)-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6′)-Ia. Escherichia coli transformed with a plasmid containing the aac(6′)-Iaj gene acquired resistance to all aminoglycosides tested except gentamicin. Of note, aac(6′)-Iaj contributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6′)-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6′)-Iaj is a functional acetyltransferase that modifies the amino groups at the 6′ positions of aminoglycosides and contributes to aminoglycoside resistance of P. aeruginosa NCGM1588, including arbekacin. Copyright © 2013, American Society for Microbiology. All Rights Reserved.


Tada T.,Japan National Institute of Infectious Diseases | Miyoshi-Akiyama T.,Japan National Institute of Infectious Diseases | Shimada K.,Japan National Institute of Infectious Diseases | Shimojima M.,BML Inc. | Kirikae T.,Japan National Institute of Infectious Diseases
Antimicrobial Agents and Chemotherapy | Year: 2013

Two novel IMP-type metallo-β-lactamase variants, IMP-43 and IMP-44, were identified in multidrug-resistant Pseudomonas aeruginosa isolates obtained in medical settings in Japan. Analysis of their predicted amino acid sequences revealed that IMP-43 had an amino acid substitution (Val67Phe) compared with IMP-7 and that IMP-44 had two substitutions (Val67Phe and Phe87Ser) compared with IMP-11. The amino acid residue at position 67 is located at the end of a loop close to the active site, consisting of residues 60 to 66 in IMP-1, and the amino acid residue at position 87 forms a hydrophobic patch close to the active site with other amino acids. An Escherichia coli strain expressing bla IMP-43 was more resistant to doripenem and meropenem but not to imipenem than one expressing blaIMP-7. An E. coli strain expressing blaIMP-44 was more resistant to doripenem, imipenem and meropenem than one expressing blaIMP-11. IMP-43 had more efficient catalytic activities against all three carbapenems than IMP-7, indicating that the Val67Phe substitution contributed to increased catalytic activities against carbapenems. IMP-44 had more efficient catalytic activities against all carbapenems tested than IMP-11, as well as increased activities compared with IMP-43, indicating that both the Val67Phe and Phe87Ser substitutions contributed to increased catalytic activities against carbapenems. Copyright © 2013, American Society for Microbiology. All Rights Reserved.


Teramura H.,Kohjin Bio Co. | Sekiguchi J.-I.,Kohjin Bio Co. | Shimojima M.,BML Inc.
Diagnostic Microbiology and Infectious Disease | Year: 2014

MRSA-chrom, a novel chromogenic screening agar medium for methicillin-resistant Staphylococcus aureus (MRSA), was developed. There were all MRSA strains recovered in 24. h as a specific blue-colored colony among 130 microbes including 42 MRSA strains. MRSA-chrom showed the highest detection ratio among 4 commercially available selective media using 50 clinical specimens. © 2014 Elsevier Inc.


Kitao T.,National Center for Global Health and Medicine | Tada T.,National Center for Global Health and Medicine | Tanaka M.,Mizuho Medy Co. R and D | Narahara K.,Mizuho Medy Co. R and D | And 4 more authors.
International Journal of Antimicrobial Agents | Year: 2012

The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa isolates producing IMP-type metallo-β-lactamases (MBLs) and aminoglycoside 6′-N-acetyltransferase [AAC(6′)-Iae] has become a serious problem in medical settings in Japan. A total of 217 MDR P. aeruginosa isolates were obtained from August 2009 to April 2010 from patients at 144 hospitals in Japan, of which 145 (66.8%) were positive for IMP-type MBLs and AAC(6′)-Iae when tested with an immunochromatographic assay. Polymerase chain reaction (PCR) showed that these isolates were also positive for blaIMP and aac(6′)-Iae genes. When these IMP-type MBL- and AAC(6′)-Iae-producing isolates were analysed by pulsed-field gel electrophoresis (PFGE), two clusters (I and II) were detected. Most of the isolates (88.3%; 128/145) were grouped under cluster I and had multilocus sequence type ST235 and serotype O11, except for one isolate that was ST991 and serotype O3. The isolates were mainly isolated from the urinary tract (82/145; 56.6%) and respiratory tract (58/145; 40.0%). The epidemiological properties of the isolates belonging to cluster I were similar to those of MDR P. aeruginosa isolates that have been previously reported in Japan. The remaining 16 isolates belonged to cluster II, had identical PFGE patterns and were multilocus sequence type ST991 and serotype O18; all of these isolates were isolated from the respiratory tract. The properties of isolates belonging to cluster II have not been previously described, indicating that a novel IMP-type MBL- and AAC(6′)-Iae producing P. aeruginosa strain is emerging in Japan. Isolates belonging to both clusters were isolated from different parts of the country. © 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.


Trademark
BML Inc | Date: 2011-07-19

Electric guitars.


Trademark
BML Inc | Date: 2012-01-19

Acoustic guitars; Electric guitars.


Trademark
BML Inc | Date: 2012-03-16

Electric bass guitars; Electric guitars.


An object of the present invention is to provide a control material which can be preferably employed in detection of 1,5-anhydro-D-glucitol and glycoalbumin, which are employed as excellent indices for diabetes. The present inventor has found that mannitol which is added to serum or plasma used as control material stabilizes 1,5-anhydro-D-glucitol and glycoalbumin present in the serum or plasma for a long period of time, and that the object can be attained through provision of (1) an agent for stabilizing control material, the agent being composed of mannitol and (2) control material containing mannitol and 1,5-anhydro-D-glucitol.


An object of the present invention is to provide a control material which can be preferably employed in detection of 1,5-anhydro-D-glucitol and glycoalbumin, which are employed as excellent indices for diabetes. The present inventor has found that mannitol which is added to serum or plasma used as control material stabilizes 1,5-anhydro-D-glucitol and glycoalbumin present in the serum or plasma for a long period of time, and that the object can be attained through provision of (1) an agent for stabilizing control material, the agent being composed of mannitol and (2) control material containing mannitol and 1,5-anhydro-D-glucitol.

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