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Zhang H.,Shandong University | Huang S.,Bloomage Freda Biopharm Co. | Yang X.,Shandong University | Zhai G.,Shandong University
European Journal of Medicinal Chemistry

Hyaluronic acid (HA) is a mucopolysaccharide acid composed of repeating disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine. Based on numerous characteristics such as viscoelastic properties, water-binding ability, biocompatibility and non-immunogenicity, HA has been approved by FDA for biological and medical applications. In addition, multifarious receptors of HA like CD44, RHAMM and TSG6 are over-expressed on the surface of malignant cells, which play important roles in targeting ability. Bioconjugates linking drugs to HA could improve solubility, prolong half-life, provide active targeting capability and then increase the bioavailability of these coupled drugs by pro-drug strategy. Therefore, a large number of HA-drug bioconjugates have been studied. The purpose of this review was to summarize these HA-drug bioconjugates and further discuss synthetic methods and the relevant application in pharmaceuticals. © 2014 Elsevier Masson SAS. All rights reserved. Source

Bloomage Freda Biopharm Co. | Date: 2016-03-31

Glycol ether; Protein for industrial use; Sodium; Sodium hyaluronate for use in the manufacture of pharmaceuticals; Antioxidants for use in the manufacture of cosmetics; Antistatic preparations, other than for household purposes; Carbonic hydrates; Chemical reagents, other than for medical or veterinary purpose; Chemicals for industrial purposes; Salts for industrial purposes.

Bloomage Freda Biopharm Co. | Date: 2012-06-13

Industrial chemicals; Chemical reagents, other than for medical or veterinary purpose; Agar-agar; Protein in raw material form for scientific and medical research; Enzymes derived from biotechnological processes for use in industry; Cellulose; Acetic acid; Adipic acid; Benzoic acid; Sodium hyaluronate.

Bloomage Freda Biopharm Co. | Date: 2012-11-29

The present invention provides a

Guo X.,Shandong University | Guo X.,Bloomage Freda Biopharm Co. | Shi Y.,Bloomage Freda Biopharm Co. | Sheng J.,Shandong University | Wang F.,Shandong University

Hyaluronidases are a family of enzymes that degrade hyaluronic acid (hyaluronan, HA) and widely used in many fields. A hyaluronidase producing bacteria strain was screened from the air. 16S ribosomal DNA (16S rDNA) analysis indicated that the strain belonged to the genus Bacillus, and the strain was named as Bacillus sp. A50. This is the first report of a hyaluronidase from Bacillus, which yields unsaturated oligosaccharides as product like other microbial hyaluronate lyases. Under optimized conditions, the yield of hyaluronidase from Bacillus sp. A50 could reach up to 1.5×104 U/mL, suggesting that strain A50 is a good producer of hyaluronidase. The hyaluronidase (HAase-B) was isolated and purified from the bacterial culture, with a specific activity of 1.02×106 U/mg protein and a yield of 25.38%. The optimal temperature and pH of HAase-B were 44°C and pH 6.5, respectively. It was stable at pH 5-6 and at a temperature lower than 45°C. The enzymatic activity could be enhanced by Ca2+, Mg2+, or Ni2+, and inhibited by Zn2+, Cu2+, EDTA, ethylene glycol tetraacetic acid (EGTA), deferoxamine mesylate salt (DFO), triton X-100, Tween 80, or SDS at different levels. Kinetic measurements of HAase-B towards HA gave a Michaelis constant (Km) of 0.02 mg/mL, and a maximum velocity (Vmax) of 0.27 A232/min. HAase-B also showed activity towards chondroitin sulfate A (CSA) with the kinetic parameters, Km and Vmax, 12.30 mg/mL and 0.20 A232/ min respectively. Meanwhile, according to the sequences of genomic DNA and HAase-B's part peptides, a 3,324-bp gene encoding HAase-B was obtained. © 2014 Guo et al. Source

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