Blood Group Reference Laboratory

Shanghai, China

Blood Group Reference Laboratory

Shanghai, China

Time filter

Source Type

Guo Z.,Clinical Laboratory | Wang C.,Clinical Laboratory | Yan K.,Clinical Laboratory | Xie J.,Clinical Laboratory | And 5 more authors.
Transfusion | Year: 2013

Background: This study aimed to analyze the mutation spectrum of the JK-null phenotype in the Chinese population. The JK gene encoding the Kidd blood group antigen protein and JK*A/JK*B polymorphism caused by a G-to-A mutation at nt838 are well described. However, the molecular basis of the JK-null phenotype in Chinese populations remains unclear. Study Desing and Methods: Sixteen unrelated JK-null phenotype donors detected by red blood cell urea lysis resistance assay of 201,194 Chinese blood donors were confirmed in serologic agglutination tests. JK-null alleles were analyzed by MnlI polymerase chain reaction-restriction fragment length polymorphism and sequencing of all JK gene coding regions. Results: In addition to the well-known Polynesian JK-null allele JK*B(IVS5-1g>a) and two alleles discovered in Taiwan, JK*B(896G>A) and JK*B(222C>A), seven JK-null allele types were detected in this study including four novel JK-null alleles: a nonsense mutation, JK*B(512G>A); two types of missense point mutations, JK*B(536C>G) and JK*B(437T>C); and a splice mutation, JK*A(IVS8+5g>a), resulting in skipping of Exon 8. Conclusion: This study demonstrates the frequency and heterogeneity of the JK-null phenotype in Chinese populations. Based on our findings, the mechanisms underlying the Chinese Jk(a-b-) phenotype are quite different from other ethnic groups. The two most common types of JK-null alleles were JK*B(IVS5-1g>a) and JK*B(896G>A) in Chinese persons. Four novel JK-null alleles were noted to be associated with the Jk(a-b-) phenotype. © 2012 American Association of Blood Banks.


Chang X.-R.,Shandong Mental Health Center | Wang L.,Shandong University | Li J.,Blood Group Reference Laboratory | Wu D.-S.,Shandong University
Brain Research | Year: 2016

The present study investigated the antidepressant potential of curcumin in olfactory bulbectomy and forced swimming test models of depression in male albino rats under chronic treatment. The experimental animals were divided into four groups, and curcumin was administered for 45 days. Our results showed that the curcumin significantly reduced olfactory bulbectomy-induced behavioral abnormalities including deficits in step-down passive avoidance, increased activity in the open area and immobility time. Chronic administration of curcumin significantly reversed levels of 3, 4-dihydroxyphenylacetic acid, noradrenaline, serotonin and 5-hydroxyindoleacetic acid in the hippocampus region of male albino rats. Also, curcumin normalizes the levels of dopamine, noradrenaline, and 5-hydroxyindoleacetic acid in the frontal cortex of rats. Taking all these results together, it may suggest that curcumin is potent compound acting against the depression in the male albino rats. © 2016, Elsevier B.V. All rights reserved.


Jiao W.,The Peoples Hospital Of Guanqxi Zhuang Autonomous Region | Liao X.,The Peoples Hospital Of Guanqxi Zhuang Autonomous Region | Li H.,The Peoples Hospital Of Guanqxi Zhuang Autonomous Region | Lan J.,The Peoples Hospital Of Guanqxi Zhuang Autonomous Region | And 8 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

There are little data available regarding the frequencies of the blood group antigens other than ABO and RhD in the Chinese Zhuang and Dong population. Knowledge of the antigen frequencies is important to assess risk of antibody formation and to guide the probability of finding antigen-negative donor blood, which is especially useful when blood is required for a patient who has multiple red cell alloantibodies. The aim of this study is sought to massively screen for rare blood donors with antigen-negative (e.g. Fy (a-), s-, k-, Di (b-) and Js (b-)) in an ethnic Zhuang and Dong population of China, for providing precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions. Finally, inheritance of the Fy (a-), s-, k-, Di (b-) and Js (b-) phenotypes was investigated by pedigree analysis. We screened for Fy (a-), s-, k-, Di (b-) and Js (b-) phenotypes in blood donors by multiplex polymerase chain reaction. The rare phenotypes Fy (a-), s-, k-, Di (b-) and Js (b-) were verified by flow cytometry and sequencing analysis. The results indicated that there are five Fy (a-), three s (-), two Di (b-) in 4490 Zhuang random samples and three Fy (a-) in 1927 Dong random samples. In conclusion, this study is the first small step to create a rare donor data bank for Chinese Zhuang and Dong population and to prepare antigen negative compatible blood to patients with multiple alloantibodies. © 2015, Int J Clin Exp Med. All rights reserved.


He Y.-L.,East China Normal University | Gao H.-H.,Blood Group Reference Laboratory | Ye L.-Y.,Blood Group Reference Laboratory | Guo Z.-H.,Blood Group Reference Laboratory | And 2 more authors.
Transfusion Medicine | Year: 2013

Objectives/Aims: This work aims to develop a multiplex polymerase chain reaction combined with DNA pooling for mass screening for rare blood types. Background: The differences in most blood group antigens are associated with single-nucleotide polymorphisms (SNPs), which are used in detecting blood antigen expression at the molecular level. However, all existing sequence-specific primers polymerase chain reaction (PCR-SSP) assays for blood typing genotype one or several SNPs individually. DNA pooling is a way that reduces the amount of genotyping required. Methods: A sensitive multiplex PCR-SSP assay testing pooled DNA was established to detect the rare Fyb and S alleles. It was applied to screen a total of 4490 donor samples via testing 898 DNA pools. The samples in the positive pools were further tested individually. Then the positive samples, including Fy(a-b+)/Fy(a+b+) and S+s-/S+s+ genotypes, were tested via two PCR-SSP assays for alleles Fya and s. The rare genotypes Fy(a-b+) and S+s- were verified using serologic tests and sequencing analysis. Results: Two hundred and fifty-four donors were tested positive for the Fyb allele, whereas 101 donors were positive for the S allele. Among the 254 Fy(b+) donors, 5 were Fy(a-b+) and 249 were Fy(a+b+). Among the 101 S+ donors, 3 were S+s- and 98 were S+s+. The rare Fyb and S alleles comprised 2·28 and 1·16%, respectively. The PCR-SSP assays were confirmed by sequencing analysis and serological test. Conclusion: A multiplex PCR assay was combined with DNA pooling to reduce the number of tests required, making large-scale screening feasible. © 2012 The Authors. Transfusion Medicine © 2012 British Blood Transfusion Society.


PubMed | The Peoples Hospital of Guanqxi Zhuang Autonomous Region Nanning, Blood Group Reference Laboratory and East China Normal University
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

There are little data available regarding the frequencies of the blood group antigens other than ABO and RhD in the Chinese Zhuang and Dong population. Knowledge of the antigen frequencies is important to assess risk of antibody formation and to guide the probability of finding antigen-negative donor blood, which is especially useful when blood is required for a patient who has multiple red cell alloantibodies. The aim of this study is sought to massively screen for rare blood donors with antigen-negative (e.g. Fy (a-), s-, k-, Di (b-) and Js (b-)) in an ethnic Zhuang and Dong population of China, for providing precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions. Finally, inheritance of the Fy (a-), s-, k-, Di (b-) and Js (b-) phenotypes was investigated by pedigree analysis. We screened for Fy (a-), s-, k-, Di (b-) and Js (b-) phenotypes in blood donors by multiplex polymerase chain reaction. The rare phenotypes Fy (a-), s-, k-, Di (b-) and Js (b-) were verified by flow cytometry and sequencing analysis. The results indicated that there are five Fy (a-), three s (-), two Di (b-) in 4490 Zhuang random samples and three Fy (a-) in 1927 Dong random samples. In conclusion, this study is the first small step to create a rare donor data bank for Chinese Zhuang and Dong population and to prepare antigen negative compatible blood to patients with multiple alloantibodies.


PubMed | Blood Group Reference Laboratory
Type: Journal Article | Journal: Transfusion | Year: 2013

This study aimed to analyze the mutation spectrum of the JK-null phenotype in the Chinese population. The JK gene encoding the Kidd blood group antigen protein and JK*A/JK*B polymorphism caused by a G-to-A mutation at nt838 are well described. However, the molecular basis of the JK-null phenotype in Chinese populations remains unclear.Sixteen unrelated JK-null phenotype donors detected by red blood cell urea lysis resistance assay of 201,194 Chinese blood donors were confirmed in serologic agglutination tests. JK-null alleles were analyzed by MnlI polymerase chain reaction-restriction fragment length polymorphism and sequencing of all JK gene coding regions.In addition to the well-known Polynesian JK-null allele JK*B(IVS5-1g>a) and two alleles discovered in Taiwan, JK*B(896G>A) and JK*B(222C>A), seven JK-null allele types were detected in this study including four novel JK-null alleles: a nonsense mutation, JK*B(512G>A); two types of missense point mutations, JK*B(536C>G) and JK*B(437T>C); and a splice mutation, JK*A(IVS8+5g>a), resulting in skipping of Exon 8.This study demonstrates the frequency and heterogeneity of the JK-null phenotype in Chinese populations. Based on our findings, the mechanisms underlying the Chinese Jk(a-b-) phenotype are quite different from other ethnic groups. The two most common types of JK-null alleles were JK*B(IVS5-1g>a) and JK*B(896G>A) in Chinese persons. Four novel JK-null alleles were noted to be associated with the Jk(a-b-) phenotype.

Loading Blood Group Reference Laboratory collaborators
Loading Blood Group Reference Laboratory collaborators