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Oliveira do Hospital, Portugal

Tullio V.,University of Turin | Mandras N.,University of Turin | Scalas D.,University of Turin | Allizond V.,University of Turin | And 6 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2010

The influence of caspofungin on polymorphonuclear leukocyte (PMN) phagocytosis and intracellular killing of Candida albicans was investigated. Caspofungin, at all of the concentrations tested (2, 3.2, and 8 μg/ml), significantly increased intracellular killing by PMNs through its direct action on both yeast cells and PMNs, indicating the potential ability of caspofungin to synergize with phagocytes for candidal killing. Caspofungin may therefore constitute an effective therapeutic option for the treatment of invasive fungal infections, including those refractory to conventional treatment with azole agents. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Source

Uzun B.,Blood Center | Gungor S.,Ataturk Training and Research Hospital | Demirci M.,Ataturk Training and Research Hospital
Transfusion and Apheresis Science | Year: 2013

Background: The most frequently encountered complication of blood transfusion is transfusion transmissible infections. Screening of transfusion transmissible infections are for safe blood transfusions, the results provide a crude idea about seropositivity rates of regions. Objective: The present study aimed to investigate distributions of transfusion transmissible infection seroprevalences in years and according to gender through medical records, and to define the regional data, retrospectively. Methods: 80.454 Blood donors, applied to our center between dates August 2004 and December 2010, were investigated by HBsAg, anti-HVC, anti-HIV ELISA (Abbott, AXSYM) and RPR methods. Results: Out of 80.454 donors, 7.321 (9.1%) were females, 73.133 (90.0%) were males. Age range of donors was 18-64. years (mean 41. years). While 61.950 (77%) of donors were voluntary, 18.504 (23%) were familial/replacement donors. 1.405 units of blood out of 80.454 were disposed, because one of infection parameters was positive. 45 units (3.2%) of disposed blood were from females, the rest belonged to male donors (1.360 units; 96.8%). HBsAg was positive in 1.054 donors (1.31%), whereas positivities of anti-HCV, anti-HIV and RPR were 312 (0.38%), 2 (0.002%) and 39 (0.04%), respectively. Conclusions: Seropositivity was determined in accordance with national data, but was at lower limits. Seropositivity rates in years differed, but neither regularly increases nor decrease was observed. When all positivities were investigated according to genders, positivity in HBsAg and VDRL tests were significantly high in male donors. © 2013 Elsevier Ltd. Source

Galanakis D.K.,State University of New York at Stony Brook | Neerman-Arbez M.,University Hospitals Geneva Medical Center | Kudryk B.,Blood Center | Henschen A.,UCI
Blood Coagulation and Fibrinolysis | Year: 2010

Reported evidence of a role in fibrinolysis by fibrinopeptide (Fp)B-dependent intermolecular fibrin polymerization contacts and of reversed FpA/FpB release sequence from fibrinogen Kingsport led us to investigate the fibrinolytic properties of Kingsport clots. Clot lysis was induced by either plasmin (pH 7.4) or by a mixture of plasminogen and recombinant tissue plasminogen activator and measured by lysis time and by turbidity (350 nm) time course. Clots were formed by thrombin from plasminogen-free fibrinogen (pH 7.4, 8 mmol/l CaCl2), with or without 40 nmol/l factor XIII or 20% afibrinogenemic plasma. Displaying no differences from corresponding normal controls were (a) lysis of repolymerized fibrin clots, and (b) chromogenic measurements of fibrin-stimulated Glu-plasminogen activation by recombinant tissue plasminogen activator. By contrast, thrombin-induced fine and coarse network clots (n = 7) displayed faster turbidity loss than corresponding normal controls and shorter lysis times ranging 31-55% of controls. Comparison of clots of fibrinogen fractions lacking approximately 90% of their alpha chain carboxyl terminal regions, n = 2, also displayed faster plasmin-induced lysis than corresponding controls. To assess the role of FpB release-dependent intermolecular polymerization contacts, clots were prepared in the presence of three molar excess antiβ 15-42 immunoglobulin G, n = 2, and displayed no differences in plasmin-induced lysis from nonimmune immunoglobulin G controls. The reversed FpA/FpB release sequence from Kingsport fibrinogen resulted in clots with decreased resistance to plasmin. We suggest that both markedly slow polymerization and decreased plasmin resistance played causative roles in the hemorrhagic diathesis associated with this dysfibrinogen. © 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins. Source

Uzun B.,Blood Center | Gungor S.,Ataturk Training and Research Hospital | Demirci M.,Ataturk Training and Research Hospital
Transfusion and Apheresis Science | Year: 2014

Background: According to our center's experiences familial/replacement donors (FRDs) frequently donate blood for the first time in their lives. Therefore, results of infection parameters are expected to be different voluntary donors (VDs), at similar rates to the population. The present study aimed to investigate if there were any difference in VDs and FRDs in terms of infection parameters. Objective: The blood donation records over 6 years (2004-2010) were reviewed, retrospectively. HBsAg, anti-HCV, anti-HIV screening tests were performed by ELISA and syphilis screening was performed by the RPR method. Material and Methods: Out of 71.217, 16.727 donors donated whole blood as FRD. Among the whole blood donated by FRD, the positives for HBsAg, anti-HCV and RPR were 1.23%, 0.37%, and 0.07%, respectively. Confirmed anti-HIV screening test was not observed in this group. Positivities for HBsAg, anti-HCV, anti-HIV and RPR in VD were 1.36%, 0.42%, 0.004%, and 0.04%, respectively. Results: When FRD and VD were analyzed statistically, HBsAg rates were significantly higher among FRD in the years 2004, 2007 and 2008, whereas they were significantly high among VD in year 2005. HBsAg rates stated in the years 2006-2009 were insignificant. Significantly high results were observed in HCV rates in the year 2005 among VD, whereas insignificant levels were observed in other years. HIV rates were insignificant among VD in the years 2004 and 2005, confirmed positivity was established in only one patient. Values in all years in RPR rates were statistically insignificant. Grouping donors as replacement and voluntary has no importance in infection parameters. Conclusion: Grouping donors as replacement and voluntary has no importance in infection parameters. Appropriate donor inquiries and screening of infection parameters by reliable proven tests preserve their significances. © 2014 Elsevier Ltd. Source

Canatan D.,Suleyman Demirel University of Turkey | Ozsancak A.,Blood Center
Asian Journal of Transfusion Science | Year: 2010

Background : Donor recruitment programs differ in countries depending on local conditions and causes. Regularly voluntary blood donation rate should be 5% of the population but it is extremely low in Turkey. In 1998, "Thalassemia flowers don't fade" campaigning was started to get regular voluntary blood for patients with thalassemia. We would like to present results of our campaigning. Materials and Methods: The Thalassemia center was established in Antalya on the 1 st June 1994 by Ministry of Health, Turkey, because the incidence of thalassemia is very high in the Antalya region. A total of 388 patients with thalassemia were followed up regularly in the center. The annually blood requirement was approximately 5000 units per year. In 1998, a new program of blood donation for patients with thalassemia called "BLOOD MOTHER and BLOOD FATHER" was started with the support of Governor of Antalya and health management system in Antalya. Results : Between year 1998 and 2006, a total 3000 voluntary blood donors between age 18 and 65 years, of which 2160 males (72%) and 840 were females (28%), had participated in this program. Conclusion: "Blood Mother and Blood Father" campaign was successful donor recruitment program for thalassemic care. After 2006, this program is now adopted and run by Turkish Red Crescent and Thalassemia Federation of Turkey for all thalassemics in Turkey. Source

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