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Jargar J.G.,Environmental Health Research Unit | Dhundasi S.A.,Environmental Health Research Unit | Das K.K.,Blde University Shri Bmpatil Medical College
Biomedicine (India) | Year: 2014

Introduction: There is growing evidence that oxidative stress contributes to the pancreatic islet P-cell destruction in diabetes. It seems that nickel induces glucose deregulation through reactive oxygen species (ROS) pathway. Since vitamin E is known to protect the cells from oxidative damage due to its potent antioxidant properties.Aim: Role of a-tocopherol on blood glucose regulation in diabetic rats exposed to nickel sulfate were investigated.Materials and Methods: Group I served as normal control. Diabetes was induced in male albino rats (180-200g b.wt) with alloxan monohydrate (15mg/100g b.wt, i.p.). Group II diabetic control, Group III (diabetic + nickel sulfate, 2mg/100gb.wt, i.p.), Group IV (diabetic + a-tocopherol, 10mg/100gb.wt, i.m.) and Group V (diabetic + nickel sulfate+α-tocopherol).Results: The authors found that body weight gain, hepato and renal somatic index were significantly improved in hyperglycemic rats due to a-tocopherol supplementation. Chronic blood glucose level, oral glucose tolerance test (OGTT), serum nitric oxide (NO) and vitamin E levels were significantly increased in both diabetic and nickel sulfate exposed diabetic rats after a-tocopherol supplementation.Conclusion: a-Tocopherol supplementation exerts a protective influence on blood glucose regulation in diabetes and nickel sulfate exposed diabetes by attenuating hyperglycemia-mediated oxidative stress and antioxidant competence.


Chadchan K.S.,P.A. College | Das S.N.,P.A. College | Das K.K.,Blde University Shri Bmpatil Medical College
Biomedicine (India) | Year: 2014

Introduction: Heavy metals like nickel may be considered as one of the important industrial hazard which is found to induce diabetes mellitus among people who are exposed to it. It is found to alter the insulin response. Objectives: To evaluate the glucose reduction capabilities of aqueous extract of fenugreek (Trigonella foenum graecum) leaves and its interaction with nickel at pH 7.0 and 9.0 in-vitro. Materials and Methods: Spectral analysis of Ni (II) alone and in presence of fenugreek extract was recorded at pH 7.0 and pH 9.0 using UV-Vis spectrophotometer at room temperature of 25°C. Glucose reduction capabilities of aqueous extract of fenugreek leaves alone and in supplementation with nickel sulphate were also evaluated at pH 7.0 and 9.0 by glucose oxidase method. Results: The λmax value of Ni showed hypsochromic shift from 393.5 and 396.5 to 323.5 and 323.0 at pH 7.0 and pH 9.0 respectively in the presence of aqueous fenugreek extract. The aqueous extract of fenugreek leaves has produced significant reduction in the glucose concentration at pH 7.0 and pH 9.0 even in presence of heavy metal like Ni (II) in-vitro. Conclusion: The aqueous extract of fenugreek leaves is capable to change the chemical behaviour of heavy metals like Ni. The study also revealed a strong glucose reducing properties of fenugreek with or without nickel supplementation in vitro. Interestingly such glucose reducing characteristics were found to be higher in alkaline medium (pH 9.0).


Basundra S.,Maulana Azad Medical College | Hiremath R.N.,Blde University Shri Bmpatil Medical College
Journal of Krishna Institute of Medical Sciences University | Year: 2016

Emerging infectious diseases comprise a substantial proportion of global morbidity and mortality. The world has been hit by Zika virus (ZIKV) after it was able to surmount an effective public health response for its control. ZIKV disease is an emerging mosquito-borne disease which occurred as large outbreaks inYap since 2007, Polynesia in 2013 and Brazil in 2015. ZIKV infection in pregnant women has been observed to be associated with congenital microcephaly with neurological and autoimmune sequelae in general population of Brazil. The incubation period of ZIKV varies from few days to weeks. Only 20% of infected cases have symptoms like any other arboviral illness. ZIKV is diagnosed using RT-PCR (reverse transcriptase-polymerase chain reaction) and virus isolation from blood samples. The treatment comprises of relief of symptoms by conservative management with no specific vaccine being available. The prevention and control of ZIKV is based on reduction of vector density by Integrated Vector Management and personal protection measures. As per Indian scenario, Ministry of Health had issued guidelines based on effective surveillance, risk communication, laboratory and travel regulations. Approaches to such a potential global health security threat should be consistent, proactive, and should involve coordinated, multi-pronged, multilateral collaborative efforts since the concern is at the highest and immediate because of Global epidemic, Rio de Janeiro Olympic Games starting from Aug 5-21, 2016 and strong association with microcephaly. Most importantly the need of the hour is the development of vaccine for protection especially the young women who are in the reproductive age groups. The research for which is ongoing as far as the current situation of global epidemic response is concerned. © Journal of Krishna Institute of Medical Sciences University.


Hattiwale H.M.,Al Ameen Medical College | Hattiwale S.H.,Al Ameen Medical College | Dhundasi S.A.,Al Ameen Medical College | Das K.K.,Blde University Shri Bmpatil Medical College
Biomedicine (India) | Year: 2013

Introduction: Obesity is a rapidly growing health problem across the globe. Elevated body mass index (BMI) is a well-established etiological factor of cardiovascular diseases. Aim: This study was designed to evaluate the influence of body mass index and waist to height ratio which are known predictors of cardiovascular diseases, with % recovery heart rate in male adult subjects of Bijapur, Karnataka, India. Methods: 52 adult males belonging to mean age group of 24.16 ± 8.12 was selected as subjects from the general population of north Karnataka, randomly for this study. The BMI, W/Ht and WHR were calculated. Subjects performed submaximal exercise test by using cycle ergometer. Heart rate and blood pressure were measured before exercise (pre-exercise), immediately after exercise (peak-exercise) and 2 minutes after cessation of exercise (post-exercise) and percent recovery of heart rate was calculated. Results: There was a statistically significant positive correlation between BMI, waist hip ratio, waist to height ratio with mean arterial pressure, and a significant negative correlation was found between waist to height ratio and percent recovery heart rate. Conclusions: This study concludes that WHtR may be considered as an important anthropometrical tool which correlates with cardiovascular fitness among young male adults of Bijapur. This anthropometrical parameter may also be used to evaluate metabolic syndrome or insulin resistance diabetic status of an individual.


Pati S.G.,Blde University Shri Bmpatil Medical College | Dhanakshirur G.B.,Blde University Shri Bmpatil Medical College | Aithala M.R.,Blde University Shri Bmpatil Medical College | Naregal G.,Blde University Shri Bmpatil Medical College | Das K.K.,Blde University Shri Bmpatil Medical College
Journal of Clinical and Diagnostic Research | Year: 2014

Background and Objectives: Hypertension, especially in elderly is a strong risk factor for cardiovascular mortality and morbidity. Oxidative stress has been implicated as one of the underlying cause of hypertension. Yoga has been found to control hypertension in the elderly, but the underlying benefits of mechanism in relation to oxidative stress regulation remains unclear. The purpose of the study was to investigate the effect of yoga on oxidative stress in elderly with Grade-I hypertension. Methods: An open parallel-arm randomised controlled study was conducted at BLDE University's Shri B.M.Patil Medical College, Hospital and Research Centre, India on elderly male individuals with Grade-I hypertension (n=57, age 60-80 years). Study (Yoga) group was assigned for yoga intervention and control group for walking for one hour in the morning for six days in a week for three months under the supervision of yoga instructor and physical training instructor respectively. Serum malondialdehyde (MDA) as an indicator of oxidative stress and antioxidants such as serum superoxide dismutase (SOD), reduced glutathione (GSH) and vitamin C levels were estimated. Results: Yoga practice for three months has significantly reduced serum MDA level (p<0.001), and enhanced antioxidants level such as SOD activity (p=0.007), serum GSH (p=0.002) and vitamin C (p=0.002). In the control group, we observed a significant increase in serum MDA level (p=0.04) and reduction in serum vitamin C level (p=0.015) with no significant difference in the SOD activity and GSH level. Conclusion: These findings suggest that yoga is an effective means to reduce oxidative stress and to improve antioxidant defense in elderly hypertensive individuals.

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