Numazu, Japan
Numazu, Japan

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Patent
Bl Co. and Arnotech Co. | Date: 2013-04-04

Definitive diagnosis and early start of treatment cannot be made for tuberculosis since conventional methods for detecting a Mycobacterium tuberculosis complex require a long time plus enormous labor and expense. Because detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of MPT64, there is a risk infection with the Mycobacterium tuberculosis complex will be missed. The present method and a kit address these long-standing needs by more rapidly and conveniently detecting a Mycobacterium tuberculosis complex, without culturing a biological sample containing the Mycobacterium tuberculosis complex, in which a biological sample is subjected to a heat-treatment so as to extracellularly secrete a Mycobacterium tuberculosis complex-specific secretory protein, particularly, MPB64, and subjecting the resulting treated sample to an immunological measurement/assay.


Conventional methods for detecting a Mycobacterium tuberculosis complex require a long time and also require enormous labor and expense. Thus, definitive diagnosis and early start of treatment cannot be made for tuberculosis, and there has been a demand for a rapid detection method. The detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of MPT64. As a result, infection with the Mycobacterium tuberculosis complex might be missed. Thus, there has been a need for a more reliable detection method. The present invention provides a method and a kit for more rapidly and conveniently detecting a Mycobacterium tuberculosis complex without culturing a biological sample containing the Mycobacterium tuberculosis complex, comprising subjecting the biological sample to a heat-treatment so as to extracellularly secrete a Mycobacterium tuberculosis complex-specific secretory protein, particularly, MPB64, and subjecting the resulting treated sample to an immunological assay.


The present invention provides a method for specifically detecting a Mycobacterium tuberculosis complex-specific secretory protein MPT64 antigen in a biological sample, whereby diagnosis of infection with Mycobacterium tuberculosis is carried out rapidly and safely with higher accuracy than before. An antibody that recognizes an epitope for MPB64 located in any one of amino acid sequences of SEQ ID NOS: 2 to 4, particularly a monoclonal antibody was obtained. Thus, an immunoassay using the antibody, particularly a sandwich immunoassay using first and second antibodies to MPB64, particularly an immunochromatographic assay and an immunochromatographic test strip are provided. A biological sample can be rapidly subjected to the immunoassay without culturing or after culturing for a time before Mycobacterium tuberculosis complex bacteria in the sample substantially start to grow. The biological sample may be pretreated by treatment for inactivation of Mycobacterium tuberculosis, or treatment by dispersion or solubilization.


Conventional methods for detecting a Mycobacterium tuberculosis complex require a long time and also require enormous labor and expense. Thus, definitive diagnosis and early start of treatment cannot be made for tuberculosis, and there has been a demand for a rapid detection method. The detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of MPT64. As a result, infection with the Mycobacterium tuberculosis complex might be missed. Thus, there has been a need for a more reliable detection method. The present invention provides a method and a kit for more rapidly and conveniently detecting a Mycobacterium tuberculosis complex without culturing a biological sample containing the Mycobacterium tuberculosis complex, comprising subjecting the biological sample to a heat-treatment so as to extracellularly secrete a Mycobacterium tuberculosis complex-specific secretory protein, particularly, MPB64, and subjecting the resulting treated sample to an immunological assay.


[Problem] An object of the present invention is to provide a detection marker that can simply and rapidly detect Mycoplasma pneumoniae, which is a pathogen of mycoplasma pneumonia, at a high sensitivity, a specific antibody against the marker, and also an immunological detection method and a kit containing the antibody. [Solution] Infection with Mycoplasma pneumoniae can be rapidly and specifically diagnosed by producing an antibody specifically reactive to P30 protein of Mycoplasma pneumoniae and performing an immunological assay using the P30 protein as a detection marker. The present invention enables easy and rapid detection of Mycoplasma pneumoniae and diagnosis of infection with the bacteria at a hospital or the like without need of specialized instruments or skilled techniques.


[Problem] An object of the present invention is to provide a detection marker that can simply and rapidly detect Mycoplasma pneumoniae, which is a pathogen of mycoplasma pneumonia, at a high sensitivity, a specific antibody against the marker, and also an immunological detection method and a kit containing the antibody. [Solution] Infection with Mycoplasma pneumoniae can be rapidly and specifically diagnosed by producing an antibody specifically reactive to P30 protein of Mycoplasma pneumoniae and performing an immunological assay using the P30 protein as a detection marker. The present invention enables easy and rapid detection of Mycoplasma pneumoniae and diagnosis of infection with the bacteria at a hospital or the like without need of specialized instruments or skilled techniques.


Trademark
BL Corporation | Date: 2014-12-16

Audio speakers; Headphones; Personal stereos.


Definitive diagnosis and early start of treatment cannot be made for tuberculosis since conventional methods for detecting a Mycobacterium tuberculosis complex require a long time plus enormous labor and expense. Because detection is difficult to perform directly from a biological sample, if the biological sample contains no or a very small amount of the Mycobacterium tuberculosis complex-specific secretory protein, there is a risk infection with the Mycobacterium tuberculosis complex will be missed. The present method and a kit address these long-standing needs by more rapidly and conveniently detecting a Mycobacterium tuberculosis complex, without culturing a biological sample containing the Mycobacterium tuberculosis complex, in which a biological sample is subjected to a heat-treatment so as to extracellularly secrete a Mycobacterium tuberculosis complex-specific secretory protein, such as at least one of MPB70, ESAT-6, or CFP-10 and subjecting the resulting treated sample to an immunological measurement/assay.


Trademark
Blinc Inc. | Date: 2010-09-21

Cosmetics and make-up.


Trademark
BL Corporation | Date: 2016-06-15

Audio speakers; Headphones; Personal stereos.

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