Bisen Biotech and Biopharma Pvt. Ltd

Gwalior, India

Bisen Biotech and Biopharma Pvt. Ltd

Gwalior, India

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Sanodiya B.,Bisen Biotech and Biopharma Pvt. Ltd. | Thakur G.,Bisen Biotech and Biopharma Pvt. Ltd. | Baghel R.,Bisen Biotech and Biopharma Pvt. Ltd. | Pandey A.,Rani Durgavati University | And 3 more authors.
Journal of Plant Protection Research | Year: 2010

Microbial preparation of herbicide is defined as bioherbicide that can control the weed. In this approach, indigenous plant pathogens isolated from weeds are cultured to produce the large numbers of infective propagules which are applied at a rate that will cause high levels of infection leading to suppression of the target weed. During the present investigation, cell free culture filtrate (CFCF) of Alternaria alternata was evaluated for its phytotoxicity against a noxious weed Lantana camara. The results of cut shoot, seedling and detached leaf bioassays revealed the presence of a toxic metabolite in the CFCF and a significant reduction in chlorophyll and protein content were also noticed. Phytotoxic moiety was further purified and characterized by using solvent partition, thin layer chromatography (TLC), FTIR and 1H NMR analysis. The acetone extract induced maximum phytotoxic damage at a concentration of 100 μg/ml and TLC purified fraction also exhibited herbicidal potential. The toxic compound was identified as tenuazonic acid upon comparison with FTIR and 1H NMR spectra. This is the first evidence that confirmed the herbicidal potential of a biorational, tenuazonic acid was produced by submerged fermentation of A. alternata.


Thakur G.S.,Tropilite Foods Pvt. Ltd | Thakur G.S.,Jiwaji University | Pandey M.,Jaipur Engineering College and Research Center | Sharma R.,Tropilite Foods Pvt. Ltd | And 7 more authors.
Physiology and Molecular Biology of Plants | Year: 2011

A protocol was developed for multiple shoot induction in a climber with highly medicinal and nutritional values i.e. Momordica balsamina (Cucurbitaceae). Effects of various factors on micropropagation were also studied. Addition of BAP at 1.0 mg/l in MS medium stimulated proliferation of shoots with 6-8 coefficients from the axillary buds. Low concentration of activated charcoal (0.2 %) promoted shoot elongation and reduced callus formation. The combination of 1.0 mg/l BAP and 1.0 mg/l KN promoted shoot elongation and proliferation. NAA proved to be more effective in inducing higher number of roots per shoot than IBA. The plantlets raised in vitro were acclimatized in green house and successfully transplanted to natural condition with 85 % survival. The present investigation showed that direct regeneration of M. balsamina offer a good opportunity to use micropropagation technique as a complementary tool for breeding and other applications. © 2011 Prof. H.S. Srivastava Foundation for Science and Society.


Dixit V.,Jiwaji University | Baghel P.,Pandit Ravishankar Shukla University | Gupta A.K.,Pandit Ravishankar Shukla University | Bisen P.S.,Jiwaji University | And 2 more authors.
International Medical Journal | Year: 2010

Background: In a view to eliminate filariasis from the globe, the World Health Organization advocated administration of single dose DEC regimen annually to endemic population. The published reports indicate success of annual single dose DEC regimens in lowering of filariasis transmission in several geographic regions. We carried out a study to find out the impact of discontinuation of annual single dose DEC administration on transmission intensity in an urban region of filarial low endemicity. Methods: The effect of single dose diethylcarbamazine regimen (6 mg/kg body wt.) in comparison to standard DEC regimen i.e; 72 mg/kg body wt (administered in 12 consecutive days) in clearing Wuchereria bancrofti microfilariae from low density microfilariae carriers (1-8 mf per 20 μl) and its impact on vector infection rate were studied in an urban region endemic for bancroftian filariasis. The efficacies of DEC regimens were determined at selected intervals by assessing the rate of successful treatment, percentage cure rate and percentage decrease in microfilariae count in treated subjects. Results: The 12 days regimen was found very effective with 100% cure rate even after 4 years of drug therapy. In subjects, who received single dose DEC regimen, the rate of successful treatment, cure rate and percent decrease in microfilariae count was significantly low when assessed 24 hour after therapy. Reexamination of subjects in this group at 6, 12, 24 and 48 months after therapy showed a sharp decline in all therapeutic indices and the microfilariae count reached pretreatment levels by 4th year of drug therapy. The single dose regimen had a marginal impact on vector infection and infectivity rates in Culex quinquefasciatus. A marked increase of vector infection and infectivity rates in parallel to human microfilaraemia rate was recorded from the same households when examined 4th year after therapy. Conclusion: The single dose 6 mg/kg body wt. DEC regimen (administered only once) failed to clear microfilariae even in a situation of filarial low endemicity and did not influence the transmission potential of C. quinquefasciatus. © 2010 Japan International Cultural Exchange Foundation.


Bisen P.S.,Jiwaji University | Bisen P.S.,Bisen Biotech and Biopharma Pvt. Ltd. | Bisen P.S.,Tropilite Foods Pvt. Ltd. | Sanodiya B.S.,Bisen Biotech and Biopharma Pvt. Ltd. | And 4 more authors.
Biotechnology Letters | Year: 2010

The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative production methods. Recently, enzymatic transesterification involving lipases has attracted attention for biodiesel production as it produces high purity product and enables easy separation from the byproduct, glycerol. The use of immobilized lipases and immobilized whole cells may lower the overall cost, while presenting less downstream processing problems, to biodiesel production. The present review gives an overview on biodiesel production technology and analyzes the factors/methods of enzymatic approach reported in the literature and also suggests suitable method on the basis of evidence for industrial production of biodiesel. © 2010 Springer Science+Business Media B.V.


Bisen P.S.,Jiwaji University | Bisen P.S.,Bisen Biotech and Biopharma Pvt. Ltd. | Bisen P.S.,Tropilite Foods Pvt. Ltd. | Baghel R.K.,Bisen Biotech and Biopharma Pvt. Ltd. | And 4 more authors.
Current Medicinal Chemistry | Year: 2010

Lentinus edodes is the first medicinal macrofungus to enter the realm of modern biotechnology. It is the second most popular edible mushroom in the global market which is attributed not only to its nutritional value but also to possible potential for therapeutic applications. Lentinus edodes is used medicinally for diseases involving depressed immune function (including AIDS), cancer, environmental allergies, fungal infection, frequent flu and colds, bronchial inflammation, heart disease, hyperlipidemia (including high blood cholesterol), hypertension, infectious disease, diabetes, hepatitis and regulating urinary inconsistancies. It is the source of several well-studied preparations with proven pharmacological properties, especially the polysaccharide lentinan, eritadenine, shiitake mushroom mycelium, and culture media extracts (LEM, LAP and KS-2). Antibiotic, anti-carcinogenic and antiviral compounds have been isolated intracellularly (fruiting body and mycelia) and extracellularly (culture media). Some of these substances were lentinan, lectins and eritadenine. The aim of this review is to discuss the therapeutic applications of this macrofungus. The potential of this macrofungus is unquestionable in the most important areas of applied biotechnology. © 2010 Bentham Science Publishers Ltd.


Debnath M.,Jaipur Engineering College and Research Center | Pandey M.,Jaipur Engineering College and Research Center | Sharma R.,Jaipur Engineering College and Research Center | Thakur G.S.,Bisen Biotech and Biopharma Pvt. Ltd. | Lal P.,Jaipur Engineering College and Research Center
Journal of Medicinal Plants Research | Year: 2010

Agave sisalana Perr. Syn (Agavaceae) is cultivated for its fiber, ornamental and medicinal value. The plant contains saponin useful for soap making and pharmacological importance. It yields a stiff fiber traditionally used in making twine, rope and also dartboards. Despite their economic importance, the Agave spp. has not been genetically improved. In vitro propagation offers an alternative to this problem through the efficient cloning of selected high-yielding "elite" plants. The present review gives a brief account on this important fiber yielding and medicinal plant with special emphasis on the secondary metabolite and in vitro propagation. © 2010 Academic Journals.

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