Birla College

Kalyān, India

Birla College

Kalyān, India
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Sharma A.,Birla College | Bhot M.,Birla College | Chandra N.,Birla College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: Bryophyllum pinnatum (Lam.) Kurz. is a medicinal herb commonly used to treat ulcers, cough, diabetes and cancer. In this study, antibacterial and antioxidant activity of aqueous and methanolic extracts of root, stem, leaf and whole plant of Bryophyllum pinnatum (Lam.) Kurz. have been evaluated. Method: In the present study, methanolic and aqueous extracts of root, stem, leaf and whole plant of Bryophyllum pinnatum have been used in the present investigation to study the superoxide, hydroxyl radical scavenging activity, iron chelating power and total antioxidant activity. Antibacterial effect was tested against six species of bacteria; three Gram-positive (Corynebacterium diphtheriae, Micrococcus luteus and Bacillus subtilis) and three Gram-negative (Alcaligenes faecalis, Bordetella bronchiseptica and Serratia marcescens). The tests were carried out using the minimum inhibitory concentration (MIC) and agar well diffusion method. Results: In our results aqueous and methanolic extracts of root, stem, leaf and whole plant of Bryophyllum pinnatum showed abilities to scavenge hydroxyl and superoxide free radicals, IC50 values for hydroxyl radical (35.48, 37.15, 31.62, 28.13 mg/ml) and (50.18, 70.79, 32.35, 25.11 mg/ml) for aqueous and methanolic extracts of root, stem, leaf and whole plant respectively. IC50 values for superoxide radical (16.21 and 16.59 mg/ml) and (19.95 and 17.78 mg/ml) for aqueous and methanolic extracts of leaf and whole plant respectively. IC50 values for iron chelating power (63.09, 25.70 and 34.54 mg/ml) for aqueous extract of root, leaf and whole plant and (40.73 and 31.62 mg/ml) for methanolic extracts of leaf and whole plant respectively. Antibacterial activity was shown by both extracts of aqueous and methanolic extract of root, stem, leaf and whole plant of Bryophyllum pinnatum (Lam.) Kurz. Conclusion: These findings suggest the excellent medicinal bioactivity of Bryophyllum pinnatum (Lam.) Kurz. and explain the popularity of this plant in the folk medicine as a remedy for different illnesses.


Sharma A.J.,Birla College | Naresh C.,Birla College
Pharmacognosy Journal | Year: 2014

Context: Bryophyllum pinnatum (Lam.) Kurz. widely used in traditional as well as folk medicinal systems are locally known as Panphuti. Traditionally, it is used for the treatment of kidney stones, urinary tract infection, burns and diarrhoea. Aims: In the present study, pharmacognostic studies of root, stem, and leaf of B. pinnatum (Lam.) Kurz. is carried out in order to standardize the plant for its phytochemical, phyiso-chemical and pharmacognostical. Materials and Methods: For standardization of plant material morphological and anatomical characterization was carried out. Physico-chemical parameters viz. ash content, extractive values, heavy metal content was carried out as per Ayurvedic Pharmacopoeia of India. Phytochemical investigations were made to know the presence of various bioactive molecules, amino acid composition. Results: Intra-stelar and extra-stelar secondary growth with wood and periderm formation along with deposition of starch grains were observed in the pith region of the root and cortical region of the stem. Calcium oxalate crystals were also present in the cortical region of the stem. Leaf lamina showed spongy parenchyma in mesophyll region and anisocytic type of stomata. Anthocyanin pigment was present below epidermal cells in petiole. Physico-chemical results can be serves as quality control data. Quantitatively carbohydrate, protein, flavonoids, phenolic compounds, saponins and pro-antocyanidins were found to be present in the root, stem and leaf part of B. pinnatum (Lam.) Kurz. Conclusion: The results of the study could be useful in setting some diagnostic indices for the identification and preparation of a monograph of the plant.


Chavan L.V.,Birla College | Anita P.,Birla College | Naresh C.,Birla College
Pharmacognosy Journal | Year: 2010

Oxidative stress arises from an imbalance in the generation and metabolisms of ROS, with more of ROS being produced, than that is metabolized. Oxidative stress plays a significant pathological role in human diseases. However, the antioxidant supplements may be used to help the human body to reduce oxidative damage. Therefore the present study evaluated the antioxidant activity of Terminalia bellerica (Gertn.) Roxb. fruits. The study was designed to evaluate and compare the antioxidant activity, total phenolics, flavonoids content of aqueous (AETB) and ethanolic (EETB) extracts of fruits. The antioxidant activity was assessed by DPPH (1,1-diphenyl-1,2-picryl hydrazyl), ABTS (2,2-Azino-bis 3-ethyl benothiazoline-6-sulfonic acid diammonium salt), nitric oxide, superoxide and hydroxyl radical scavenging assay, FRAP (Ferric Reducing Antioxidant Power), reducing power and TAC (Total antioxidant capacity). AETB has shown higher antioxidant activity (% inhibition) as compared to EETB in nitric oxide, superoxide, ABTS(2,2-Azino-bis 3-ethyl benothiazoline-6-sulfonic acid diammonium salt) radical scavenging assay with IC50 values 41.42±1.23, 892.85±2.73, 23.74±2.0 in AETB and 93.16±1.93, 3496.50±5.21, 55.89±2.21 in EETB respectively. Like antioxidant activity the reducing power increases in a dose dependent manner showing higher absorbance at 700 nm for AETB i.e. 0.95±0.03 as compared to EETB i.e. 0.43±0.01 at 500μg/ml. The FRAP values were found for AETB 1.68±0.07, EETB 1.06±0.03 and TAC values were found for AETB 2.53±0.07 and EETB 2.90±0.01. The amounts of total phenolic (TPC) and flavonoid content (TFC) were also determined. The results suggest that TPC and TFC contribute significantly to the antioxidant activity of the Terminalia bellerica (Gertn.) Roxb. Fruits. © 2010 Phcog.net.


Patil A.,Birla College | Patil D.,Smt CHM College | Ansari A.,Birla College | Koli S.,Birla College
Indian Journal of Traditional Knowledge | Year: 2013

To obtain effective drug with proven efficacy, it is necessary to establish standards through modern scientific and technical procedures which would certainly enhance the reputation and acceptance of traditional medicines. Sufoof-e-mohazzil, a unani polyherbal formulation is used by Unani physicians for treatment of obesity. The present work involves standardization of Sufoof-e-mohazzil in order to assess the quality of In-house and marketed formulations. In-house and marketed formulations were subjected to pharmacognostic studies, physico-chemical properties, phytochemical analysis and HPTLC fingerprint profile to set the standards, which can be used as reference standard to evaluate the quality control in herbal industries and to overcome batch to batch variations in traditional preparation. In the present study, the anatomical markers fibro vascular tissue, endospermic cells, vittae, pitted vessels and group of sclereids from the mesocarp were observed. The extractive values were found to be more for alcohol than water. The preliminary phytochemical analysis indicated presence of alkaloids, fats and fixed oils, essential oils, glycosides, resins and saponins. HPTLC fingerprint profile of formulations showed a characteristic pinkish red band of Thymol at Rf of 0.43.


Gupta A.D.,Birla College | Karthikeyan S.,Dr Ambedkar Government Arts College
Ecotoxicology and Environmental Safety | Year: 2016

Ni and Cr are ubiquitous pollutants in the aquatic environments. These heavy metals elicit toxicities to aquatic organisms including microbes. In this study, interaction of the two heavy metals on the toxicity in Escherichia coli (E. coli) was studied using FTIR spectroscopy. The binding of Ni(II) to E. coli was stronger than that for Cr(VI). Cr exhibited antagonistic effects in the presence of Ni in E. coli. FTIR analysis showed a decrease in lipid content in the presence of Ni and not for Cr. Further, a decrease in band area was observed in the region of 3000–2800 cm−1 and at ~1455 cm−1 due to a decrease in fatty acids and lipid molecules. The band area ratio of lipid was used to monitor the changes in fatty acids due to metal toxicity. Principle component method helps to discriminates the results between control and metal toxicities in E. coli from the FTIR data. The study shows the importance of metal interaction and its toxicity on E. coli. © 2016 Elsevier Inc.


Patil A.G.,Birla College | Joshi K.A.,Birla College | Patil D.A.,Birla College | Chandra N.,Birla College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

The use of plant as medicine is as old as human civilization. Exploration of this traditional knowledge for cures to common diseases is an attractive prospect. Cardiospermum halicacabum L commonly known as 'Balloon vine' has many associated utilities, including a remedy for joint pain. The tender, young shoots are used as a vegetable, fodder, diuretic, stomachic and rubefacient. It is used in rheumatism, lumbago, nervous diseases and as a demulcent in orchitis and in dropsy. In many regions this plant is harvested in backyards for both medicinal and food value. To get information about correct identification of the stem of this plant pharmacognostical and physico-chemical studies, as well as phytochemical analysis and HPTLC fingerprint were carried out. Anatomical studies revealed presence of covering trichomes, anomocytic stomata, pitted as well as spiral thickening of vessels and fibres. Various physico-chemical parameters such as total ash, water soluble ash, acid insoluble ash and extractive values were carried out. Preliminary phytochemical analysis and TLC showed presence of saponin, tannins, flavanoids, glycosides and cardiac glycosides. This study will provide referential pharmacognostical, physico-chemical and phytochemical information for correct identification of Cardiospermum halicacabum L. stem. © 2010 RJPBCS.


Lobo V.,Birla College | Patil A.,Birla College | Phatak A.,Birla College | Chandra N.,Birla College
Pharmacognosy Reviews | Year: 2010

In recent years, there has been a great deal of attention toward the field of free radical chemistry. Free radicals reactive oxygen species and reactive nitrogen species are generated by our body by various endogenous systems, exposure to different physiochemical conditions or pathological states. A balance between free radicals and antioxidants is necessary for proper physiological function. If free radicals overwhelm the body's ability to regulate them, a condition known as oxidative stress ensues. Free radicals thus adversely alter lipids, proteins, and DNA and trigger a number of human diseases. Hence application of external source of antioxidants can assist in coping this oxidative stress. Synthetic antioxidants such as butylated hydroxytoluene and butylated hydroxyanisole have recently been reported to be dangerous for human health. Thus, the search for effective, nontoxic natural compounds with antioxidative activity has been intensified in recent years. The present review provides a brief overview on oxidative stress mediated cellular damages and role of dietary antioxidants as functional foods in the management of human diseases.


Patil A.G.,Birla College | Koli S.P.,Birla College | Patil D.A.,Birla College | Chandra N.,Birla College
International Journal of Pharma and Bio Sciences | Year: 2010

Crataeva tapia Linn. ssp. odora (Jacob.) Almeida, a traditional medicinal plant, which is valued for its benefits in the management of urinary and inflammatory disorders. Leaves are externally rubefacient and used in rheumatism; internally they are given as febrifuge and tonic. The leaves also act as anti-periodic. The current study was therefore carried out to provide requisite pharmacognostic details about the leaves of C. tapia. Pharmacognostic evaluation included examination of morphological and microscopical characters; physicochemical properties, phytochemical analysis, fluorescence study and HPTLC finger print. The powder microscopy showed the presence of anomocytic stomata and spiral thickening. Phytochemical screening reported the presence of tannins, steroidal compounds, cardiac glycosides and alkaloids. The Rf value of 0.35 detected at 300 nm and 600 nm by qualitative densitometric HPTLC fingerprint, can be used as identifying marker for methanolic extract. The present study will provide the information with respect to identification and authentication of crude drug.


Dhaliwal M.K.,Birla College | Chandra N.,Birla College
Indian Journal of Geo-Marine Sciences | Year: 2016

Marine red yeasts were enriched and isolated using sea-water samples from different locations as well as various marine animals. Two red yeast isolates selected were studied for their carotenoids production. Two selected isolates were identified as Rhodosporidium paludigenum and Sporobolomyces novazealandicus based on their cultural, morphological and biochemical characters. The isolates were studied for their carotenoid content using HPTLC and HPLC. The Rhodosporidium paludigenum isolate was observed to produce β-carotene only, whereas isolate Sporobolomyces novazealandicus produced higher amounts of total carotenoids, with an additional pigment. © 2016, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.


PubMed | Birla College and Electronic Arts
Type: | Journal: Ecotoxicology and environmental safety | Year: 2016

Ni and Cr are ubiquitous pollutants in the aquatic environments. These heavy metals elicit toxicities to aquatic organisms including microbes. In this study, interaction of the two heavy metals on the toxicity in Escherichia coli (E. coli) was studied using FTIR spectroscopy. The binding of Ni(II) to E. coli was stronger than that for Cr(VI). Cr exhibited antagonistic effects in the presence of Ni in E. coli. FTIR analysis showed a decrease in lipid content in the presence of Ni and not for Cr. Further, a decrease in band area was observed in the region of 3000-2800cm(-1) and at ~1455cm(-1) due to a decrease in fatty acids and lipid molecules. The band area ratio of lipid was used to monitor the changes in fatty acids due to metal toxicity. Principle component method helps to discriminates the results between control and metal toxicities in E. coli from the FTIR data. The study shows the importance of metal interaction and its toxicity on E. coli.

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